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Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains

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Abstract

Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of S. cerevisiae.

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Achnowledgements

The authors thank Dr. Raphaël Duval (SRSMC-Nancy University) for facilitating imaging experiments.

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Authors and Affiliations

  1. LCPME, Laboratoire de Chimie Physique et Microbiologie pour l’Environnement, Nancy-University, CNRS, 405 rue de Vandoeuvre, 54600, Villers-lès-Nancy, France

    Joël Coulon, Ilan Thouvenin & Fadi Aldeek

  2. DCPR, Département de Chimie Physique de Réactions, Nancy-University, CNRS, 1 rue Grandville, BP 20451, 54001, Nancy, France

    Raphaël Schneider

  3. DPG, Département de Photochimie Générale, UMR CNRS 7525, Université de Haute Alsace, ENSCMu, 3 rue Alfred Werner, 68093, Mulhouse, France

    Lavinia Balan

Authors
  1. Joël Coulon
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  2. Ilan Thouvenin
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  3. Fadi Aldeek
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  4. Lavinia Balan
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  5. Raphaël Schneider
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Correspondence to Joël Coulon or Raphaël Schneider.

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Coulon, J., Thouvenin, I., Aldeek, F. et al. Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains. J Fluoresc 20, 591–597 (2010). https://doi.org/10.1007/s10895-009-0590-8

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  • DOI: https://doi.org/10.1007/s10895-009-0590-8

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