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Fluorescence Quenching to Study Protein-ligand Binding: Common Errors

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An Erratum to this article was published on 27 August 2011

Abstract

A number of recent articles, amongst others several published in the Journal of Fluorescence, use inappropriate fluorescence methodology to determine ligand binding characteristics to (mostly) proteins. In this Letter, several common pitfalls are discussed in relation to two recent publications in the Journal of Fluorescence (Wang et al. (2009) 19:801–808; Ding et al. (2009) 19:783–791). The Author hopes that this contribution helps to prevent a further spread of the incorrect methodology, and results in a reappraisal of those articles already published using similar methodology.

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Notes

  1. Many of the papers using this quenching methodology use the terms “association constant” and “binding constant” in a way that suggests they are considered to be two different constants, hence my use of these two terms here also

References

  1. Wang Z, Tan X, Chen W, Yue Q, Song Z (2009) Study of the binding behavior of lysozyme with cephalosporin analogues by fluorescence spectroscopy. J Fluoresc 19:801–808

    Article  CAS  PubMed  Google Scholar 

  2. Ding F, Liu W, Liu F, Li Z-Y, Sun Y (2009) A study of the interaction between malachite green and lysozyme by steady-state fluorescence. J Fluoresc 19:783–791

    Article  CAS  PubMed  Google Scholar 

  3. Gök E, Öztürk C, Akbay N (2008) Interaction of thyroxine with 7 hydroxycoumarin: a fluorescence quenching study. J Fluoresc 18:781–785

    Article  PubMed  Google Scholar 

  4. He Y, Wang Y, Tang L, Liu H, Chen W, Zheng Z, Zou G (2008) Binding of puerarin to human serum albumin: a spectroscopic analysis and molecular docking. J Fluoresc 18:433–442

    Article  PubMed  Google Scholar 

  5. Lu Z, Zhang Y, Liu H, Yuan J, Zheng Z, Zou G (2007) Transport of a cancer chemopreventive polyphenol, resveratrol: interaction with serum albumin and hemoglobin. J Fluoresc 17:580–587

    Article  CAS  PubMed  Google Scholar 

  6. Ran D, Wu X, Zheng J, Yang J, Zhou H, Zhang M, Tang Y (2007) Study on the interaction between florasulam and bovine serum albumin. J Fluoresc 17:721–726

    Article  CAS  PubMed  Google Scholar 

  7. Xiang G, Tong C, Lin H (2007) Nitroaniline isomers interaction with bovine serum albumin and toxicological implications. J Fluoresc 17:512–521

    Article  CAS  PubMed  Google Scholar 

  8. Xiao JB, Chen XQ, Jiang XY, Hilczer M, Tachiya M (2008) Probing the interaction of trans-resveratrol with bovine serum albumin: a fluorescence quenching study with Tachiya model. J Fluoresc 18:671–678

    Article  CAS  PubMed  Google Scholar 

  9. Yue Q, Niu L, Li X, Shao X, Xie X, Song Z (2008) Study on the interaction mechanism of lysozyme and bromophenol blue by fluorescence spectroscopy. J Fluoresc 18:11–15

    Article  CAS  PubMed  Google Scholar 

  10. Zhang H-G, Huang X, Mei P, Li K-H, Yan C-N (2006) Studies on the interaction of tricyclazole with b-cyclodextrin and human serum albumin by spectroscopy. J Fluoresc 16:287–294

    Article  CAS  PubMed  Google Scholar 

  11. Zhang Y-Z, Zhou B, Liu Y-X, Zhou C-X, Ding X-L, Liu Y (2008) Fluorescence study on the interaction of bovine serum albumin with p-aminoazobenzene. J Fluoresc 18:109–118

    Article  CAS  PubMed  Google Scholar 

  12. Zhang Y, Dong L, Li Y, Li J, Chen X (2008) Characterization of interaction between bergenin and human serum albumin in membrane mimetic environments. J Fluoresc 18:661–670

    Article  CAS  PubMed  Google Scholar 

  13. Zhang H-X, Huang X, Zhang M (2008) Thermodynamic studies on the interaction of dioxopromethazine to β-cyclodextrin and bovine serum albumin. J Fluoresc 18:753–760

    Article  CAS  PubMed  Google Scholar 

  14. Akbay N, Seferoglu Z, Gök E (2009) Fluorescence interaction and determination of calf thymus DNA with two ethidium derivatives. J Fluoresc, doi:10.1007/s10895-009-0504-9

  15. Epps DE, Raub TJ, Kézdy FJ (1995) A general, wide-range spectrofluorimetric method for measuring the site-specific affinities of drugs toward human serum albumin. Anal Biochem 227:342–350

    Article  CAS  PubMed  Google Scholar 

  16. Bi S, Ding L, Tian Y, Song D, Zhou X, Liu X, Zhang H (2004) Investigation of the interaction between flavonoids and human serum albumin. J Mol Struct 703:37–45

    Article  CAS  Google Scholar 

  17. Lakowicz JR (1999) Principles of fluorescence spectroscopy. Kluwer Academic/Plenum, New York

    Google Scholar 

  18. Chacon P, Moran F, Diaz JF, Pantos E, Andreu JM (1998) Low-resolution structures of proteins in solution retrieved from x-ray scattering with a genetic algorithm. Biophys J 74:2760–2775

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100, Copenhagen, Denmark

    Marco van de Weert

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  1. Marco van de Weert
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Correspondence to Marco van de Weert.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10895-011-0953-9.

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van de Weert, M. Fluorescence Quenching to Study Protein-ligand Binding: Common Errors. J Fluoresc 20, 625–629 (2010). https://doi.org/10.1007/s10895-009-0572-x

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