Abstract
Fluorescence techniques are widely used as sensitive detection methods in bio-analytics. The use of the bio-physical parameter fluorescence lifetime additional to the spectral characteristics of fluorescence has the potential to improve fluorescence-related detection methods in terms of selectivity in signal recognition, robustness against disturbing influences, and the accessibility of novel bio-chemical process parameters. This article describes the technical set up of a time-resolving instrument with either a fixed time-gated detection principle for improved evaluation of tissue metabolism by an online monitoring of the tissue autofluorescence or a direct fluorescence lifetime detection principle for lifetime-based fluorescent assays.
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Pfeifer, L., Stein, K., Fink, U. et al. Improved Routine Bio-Medical and Bio-Analytical Online Fluorescence Measurements Using Fluorescence Lifetime Resolution. J Fluoresc 15, 423–432 (2005). https://doi.org/10.1007/s10895-005-2634-z
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DOI: https://doi.org/10.1007/s10895-005-2634-z