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Plants on Constant Alert: Elevated Levels of Jasmonic Acid and Jasmonate-Induced Transcripts in Caterpillar-Resistant Maize

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Abstract

This study was conducted to determine if constitutive levels of jasmonic acid (JA) and other octadecanoid compounds were elevated prior to herbivory in a maize genotype with documented resistance to fall armyworm (Spodoptera frugiperda) and other lepidopteran pests. The resistant inbred Mp708 had approximately 3-fold higher levels of jasmonic acid (JA) prior to herbivore feeding than the susceptible inbred Tx601. Constitutive levels of cis-12-oxo-phytodienoic acid (OPDA) also were higher in Mp708 than Tx601. In addition, the constitutive expression of JA-inducible genes, including those in the JA biosynthetic pathway, was higher in Mp708 than Tx601. In response to herbivory, Mp708 generated comparatively higher levels of hydrogen peroxide, and had a greater abundance of NADPH oxidase transcripts before and after caterpillar feeding. Before herbivore feeding, low levels of transcripts encoding the maize insect resistance cysteine protease (Mir1-CP) and the Mir1-CP protein were detected consistently. Thus, Mp708 appears to have a portion of its defense pathway primed, which results in constitutive defenses and the ability to mount a stronger defense when caterpillars attack. Although the molecular mechanisms that regulate the constitutive accumulation of JA in Mp708 are unknown, it might account for its enhanced resistance to lepidopteran pests. This genotype could be valuable in studying the signaling pathways that maize uses to response to insect herbivores.

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Acknowledgements

This work was supported by National Science Foundation Award IOS-0641219 to DSL and a Specific Cooperative Agreement 58-6406-6-039 between Mississippi State University and USDA-ARS Corn Host Plant Resistance Research Unit.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS, 39762, USA

    Renuka Shivaji, Alberto Camas, Arunkanth Ankala & Jeff R. Wilkinson

  2. Department of Biology, University of Texas San Antonio, San Antonio, TX, 78249, USA

    Jurgen Engelberth

  3. Department of Entomology and Center for Chemical Ecology, The Pennsylvania State University, University Park, PA, 16802, USA

    James H. Tumlinson

  4. USDA-ARS Corn Host Plant Resistance Research Laboratory, Mississippi State, MS, 39762, USA

    W. Paul Williams

  5. Department of Crop and Soil Sciences, The Pennsylvania State University, University Park, PA, 16802, USA

    Dawn Sywassink Luthe

  6. Cinvestav-Unidad Irapuato, Dpto. Ingenieria Genetica, Km. 6.9 Libramiento Norte, Carr Irapuato-Leon, Gto, 36821, Mexico

    Alberto Camas

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  1. Renuka Shivaji
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Shivaji, R., Camas, A., Ankala, A. et al. Plants on Constant Alert: Elevated Levels of Jasmonic Acid and Jasmonate-Induced Transcripts in Caterpillar-Resistant Maize. J Chem Ecol 36, 179–191 (2010). https://doi.org/10.1007/s10886-010-9752-z

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