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FcγRI-Mediated Activation of Human Mast Cells Promotes Survival and Induction of the Pro-survival Gene Bfl-1

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Abstract

Activation of mast cells through either FcɛRI or FcγRI leads to release of mediators contributing to the inflammatory response. One of the biologic characteristics of mast cells in allergic pathology is that these cells have the capacity to recover and regranulate after aggregation of FcɛRI. We have previously demonstrated that the pro-survival protein A1/Bfl-1 is required for mast cells to survive IgE-mediated activation. In the present study, we have investigated whether human mast cells show similar induction of bfl-1 and activation-induced survival after aggregation of FcγRI. Human cord blood-derived mast cells were activated by aggregation of either FcɛRI or FcγRI, and activation-induced survival and induction of bfl-1 was measured. We found that aggregation of FcγRI-induced expression of bf-1 and caused a comparable activation-induced mast cell survival as FcɛRI does. These data suggests that activation through Fc-receptors contribute to mast cell survival during antibody-dependent mast cell mediated inflammatory responses.

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Acknowledgments

We wish to thank Mrs Agnetha Beinhoff for the mast cell cultures. This work was supported by the Swedish Research Council-Medicine, the Swedish Cancer Society, The Swedish Allergy and Cancer Foundation, the King Gustaf V:s 80-years Foundation, the Ollie and Elof Ericsson Foundation, Ellen, Walter, and Lennart Hesselmans foundation for scientific research, Consul Th C Berghs foundation, Karolinska Institutet, and Innoventus Project AB.

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Author notes

  1. Zou Xiang

    Present address: The Cambridge Institute for Medical Research, University of Cambridge School of Clinical Medicine, Addenbrooke’s Hospital, Cambridge, CB2 2XY, UK

Authors and Affiliations

  1. Department of Genetics and Pathology, Uppsala University, 751 85, Uppsala, Sweden

    Mats Karlberg, Zou Xiang & Gunnar Nilsson

  2. Department of Medicine, Karolinska Institutet, 171 76, Stockholm, Sweden

    Mats Karlberg & Gunnar Nilsson

  3. Clinical Immunology and Allergy, Department of Medicine, Karolinska Institutet, 171 76, Stockholm, Sweden

    Gunnar Nilsson

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  1. Mats Karlberg
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Correspondence to Gunnar Nilsson.

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Karlberg, M., Xiang, Z. & Nilsson, G. FcγRI-Mediated Activation of Human Mast Cells Promotes Survival and Induction of the Pro-survival Gene Bfl-1. J Clin Immunol 28, 250–255 (2008). https://doi.org/10.1007/s10875-007-9153-4

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  • DOI: https://doi.org/10.1007/s10875-007-9153-4

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