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Differential Contribution of the CysLTR1 Gene in Patients with Aspirin Hypersensitivity

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Abstract

In this study, we compared the roles of CysLT receptor type 1 (CysLTR1) and leukotriene C4 synthase (LTC4S) gene polymorphisms in two major aspirin-related allergic diseases, aspirin-intolerant asthma (AIA) and aspirin-induced chronic urticaria/angioedema (AICU). CysLTR1-634C>T and LTC4S-444A>C polymorphisms were genotyped and its functional effect on the promoter activity was compared. As in vivo functional study, changes of peripheral mRNA level of CysLTR1 were measured by real-time PCR before and after aspirin challenge. A significant association was found for the CysLTR1 promoter polymorphism and the AIA phenotype compared to AICU (P = 0.015). In U937 cells, the variant genotype reporter construct showed significantly higher promoter activity than the common genotype (P < 0.05). The CysLTR1 mRNA levels increased significantly after aspirin challenge in AIA patients (P = 0.013). In conclusion, the CysLTR1 polymorphism may contribute to develop to the AIA phenotype and be used as a genetic marker for differentiating two major aspirin hypersensitivity phenotypes.

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Acknowledgement

This study was supported by a grant from the Korea Health 21 R&D Project of the Ministry of Health and Welfare, Republic of Korea (03-PJ10-PG13-GD01-0002 and A050571).

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Authors and Affiliations

  1. Department of Allergy and Rheumatology, Ajou University School of Medicine, San-5, Woncheondong, Youngtonggu, Suwon, 442-721, Republic of Korea

    Seung-Hyun Kim, Eun-Mi Yang, Han-Jung Park, Young-Min Ye, Hyun-Young Lee & Hae-Sim Park

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  1. Seung-Hyun Kim
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  2. Eun-Mi Yang
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  3. Han-Jung Park
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  4. Young-Min Ye
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  6. Hae-Sim Park
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Correspondence to Hae-Sim Park.

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Kim, SH., Yang, EM., Park, HJ. et al. Differential Contribution of the CysLTR1 Gene in Patients with Aspirin Hypersensitivity. J Clin Immunol 27, 613–619 (2007). https://doi.org/10.1007/s10875-007-9115-x

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  • DOI: https://doi.org/10.1007/s10875-007-9115-x

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