Abstract
We analyzed the community structure of marine ammonia-oxidizing archaea (AOA) by sequencing the ammonia monooxygenase subunit A (amoA) gene. Cells were grown in ammonium-enriched seawater at various temperatures. Most amoA clones retrieved prior to cultivation belonged to Water Column Cluster B (WCB); however, most clones retrieved after incubation at high temperature belonged to Water Column Cluster A (WCA) or the Nitrosopumilus maritimus-like (NM) group. Some operational taxonomic units in the NM and WCA groups may be better adapted to higher temperature than those in WCB. Hence, differing temperature adaptations among AOA groups may be related to community structures and depth distributions.
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Acknowledgments
We are grateful to the captain and crew of the research vessel Tansei-maru and the research members of cruise KT08-02. In particular, we thank Mr. Hajime Itoh, Dr. Ryo Kaneko, and Dr. Susumu Yoshizawa for helping with the sampling, phylogenetic analyses, and writing this paper, respectively (Atmosphere and Ocean Research Institute, The University of Tokyo). We thank the staff and members of Marine Microbiology (Atmosphere and Ocean Research Institute, The University of Tokyo), and Hidetoshi Urakawa for providing advice.
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Ijichi, M., Hamasaki, K. Community structure of ammonia-oxidizing marine archaea differs by depth of collection and temperature of cultivation. J Oceanogr 67, 739–745 (2011). https://doi.org/10.1007/s10872-011-0066-8
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DOI: https://doi.org/10.1007/s10872-011-0066-8