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Role of Trypanosoma cruzi peroxiredoxins in mitochondrial bioenergetics

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Abstract

Trypanosoma cruzi cytosolic (TcCPx) and mitochondrial tryparedoxin peroxidase (TcMPx) play a fundamental role in H2O2 detoxification. Herein, mitochondrial bioenergetics was evaluated in cells that overexpressed TcCPx (CPx) and TcMPx (MPx) and in pTEX. In MPx, a higher expression was observed for TcCPx, and the same correlation was true for CPx. Differences in H2O2 release among the overexpressing cells were detected when the mitochondrial respiratory chain was inhibited using antimycin A or thenoyltrifluoroacetone. MPx had higher O2 consumption rates than pTEX and CPx, especially in the presence of oligomycin. In all of the cells, the mitochondrial membrane potential and the ATP levels were similar. Because of the mild uncoupling that was observed in MPx, the presence or induction of a proton transporter in the mitochondrial membrane is suggested when TcMPx is expressed at higher levels. Our results show a possible interplay between the cytosolic and mitochondrial antioxidant systems in a trypanosomatid.

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Authors and Affiliations

  1. Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Bioquímica, IB, UNICAMP, CEP: 13083-862, Campinas, São Paulo, Brazil

    Eduardo de Figueiredo Peloso, Simone Cespedes Vitor, Luis Henrique Gonzaga Ribeiro & Fernanda Ramos Gadelha

  2. Departamento de Bioquímica, Facultad de Medicina, Universidad de la República and Unidad de Biología Molecular, Instituto Pasteur Montevideo, Montevideo, Uruguay

    María Dolores Piñeyro & Carlos Robello

Authors
  1. Eduardo de Figueiredo Peloso
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  2. Simone Cespedes Vitor
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  3. Luis Henrique Gonzaga Ribeiro
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  4. María Dolores Piñeyro
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  5. Carlos Robello
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  6. Fernanda Ramos Gadelha
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Corresponding author

Correspondence to Fernanda Ramos Gadelha.

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de Figueiredo Peloso, E., Vitor, S.C., Ribeiro, L.H.G. et al. Role of Trypanosoma cruzi peroxiredoxins in mitochondrial bioenergetics. J Bioenerg Biomembr 43, 419–424 (2011). https://doi.org/10.1007/s10863-011-9365-4

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  • DOI: https://doi.org/10.1007/s10863-011-9365-4

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