Abstract
Injury to allografts during ischaemia/reperfusion contribute to the development of graft failure following transplantation with significant morbidity and mortality to patients. The development of University of Wisconsin solution has significantly improved the quality of graft preservation and transplant outcome relative to formerly used solutions such as Eurocollins. The aim of this study was to further characterize mitochondrial structural and functional alterations occurring in rat livers following cold storage and transplantation. Mitochondrial impairment after prolonged storage in Eurocollins included decreased cyt. c+c1, cyt. b and cyt. a+a3 concentration and dramatic falls in the activities of the respiratory chain enzymes ubiquinol-cyt. c oxidoreductase and cytochrome oxidase. Under the same conditions the highest hydroperoxide but lowest vitamin E concentrations were also found. Although both the Eurocollins and University of Wisconsin preservation solutions have limitations in preventing oxidative injuries following cold storage and reperfusion, our data indicate that mitochondrial impairment was higher in Eurocollins- than in University of Wisconsin-stored livers. Further improvements are necessary in maintaining the stability of mitochondria in order to optimize preservations solutions used in transplantations.
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Abbreviations
- AAPH:
-
2,2′;-azobis(amidinopropane hydrochloride)
- CD:
-
conjugated dienes; CoQ9, coenzyme Q9
- CoQ9H2 :
-
reduced coenzyme Q9
- DGF:
-
delayed graft function
- EC:
-
Euro-Collins preservation solution
- FOX2:
-
ferrous-oxide xylenol orange
- HP:
-
hydroperoxides
- HPLC:
-
high performance liquid chromatography
- IRI:
-
ischaemia/reperfusion injury
- PNF:
-
primary non-function
- ROS:
-
reactive oxygen species
- TN:
-
turnover numbers
- TPP:
-
tryphenylphosphine
- UW:
-
University of Wisconsin preservation solution
- Vit. E:
-
vitamin E
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Jassem, W., Armeni, T., Quiles, J.L. et al. Protection of mitochondria during cold storage of liver and following transplantation: comparison of the two solutions, University of Wisconsin and Eurocollins. J Bioenerg Biomembr 38, 49–55 (2006). https://doi.org/10.1007/s10863-006-9005-6
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DOI: https://doi.org/10.1007/s10863-006-9005-6