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A novel personalized 3D injectable protein scaffold for regenerative medicine

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Biomaterials should be designed to closely resemble the characteristics and functions of the native extracellular matrix to provide mechanical support and signals to direct biological events. Here we have developed a novel injectable plasma rich in growth factors (PRGF-Endoret)-based formulation that combines a thermal-denaturation step of plasma with an autologous fibrin crosslinking. Rheological and mechanical properties were evaluated. Additionally, the microstructure and biological capacity of the biomaterial was also characterized. This novel formulation exhibited ideal mechanical properties and a gel-like behavior with the ability to progressively release its growth factor load over time. The results also suggested that the novel injectable formulation is non-cytotoxic, biocompatible and suitable for cell ingrowth as it is deduced from the fibroblast proliferation within the scaffold. Finally, stimulation of both cell proliferation and matrix proteins synthesis demonstrated the regenerative potential of this autologous protein based injectable scaffold.

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Acknowledgements

This research has received specific grants from the funding agency of the Basque Country Government (Spain) under the research and development project Gaitek (IG-2015/00355) and Hazitek (ZL-2016/00411). Eduardo Anitua is the scientific director of, and Ander Pino, María Troya and Gorka Orive are researchers at BTI Biotechnology Institute, the company that has developed the PRGF-Endoret technology.

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Authors and Affiliations

  1. Foundation Eduardo Anitua, Vitoria, Spain

    Eduardo Anitua & Gorka Orive

  2. BTI—Biotechnology Institute, Vitoria, Spain

    Eduardo Anitua, Ander Pino, María Troya & Gorka Orive

  3. Dermatology Department, Ramón y Cajal Hospital, University of Alcalá, Madrid, Spain

    Pedro Jaén

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  1. Eduardo Anitua
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  2. Ander Pino
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  3. María Troya
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  4. Pedro Jaén
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  5. Gorka Orive
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Correspondence to Eduardo Anitua or Gorka Orive.

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Anitua, E., Pino, A., Troya, M. et al. A novel personalized 3D injectable protein scaffold for regenerative medicine. J Mater Sci: Mater Med 29, 7 (2018). https://doi.org/10.1007/s10856-017-6012-6

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