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Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0−1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion; however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.

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Acknowledgements

This work was supported by the Grant Agency of the Czech Republic (grants 15-05864S and 14-04790S), by the Grant Agency of the Czech Technical University in Prague (grant No. SGS14/168/OHK4/2T/17), and by the “BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University” project (CZ.1.05/1.1.00/02.0109), funded by the European Regional Development Fund. The authors thank Mrs. Ivana Zajanova (Inst. Physiol., Acad. Sci. CR, Prague) for her help with immunofluorescence staining, Mrs. Paula Solon (University of New York in Prague) and Mr. Robin Healey (Czech Technical University in Prague) for their language revision of the manuscript.

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Authors and Affiliations

  1. Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic

    Elena Filova, Marta Vandrovcova, Lubica Stankova & Lucie Bacakova

  2. Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic

    Miroslav Jelinek, Josef Zemek, Jana Houdkova,  Jan Remsa & Tomas Kocourek

  3. Czech Technical University in Prague, Faculty of Biomedical Engineering, Nam. Sitna 3105, 272 01, Kladno, Czech Republic

    Miroslav Jelinek,  Jan Remsa & Tomas Kocourek

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  1. Elena Filova
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Correspondence to Marta Vandrovcova.

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Filova, E., Vandrovcova, M., Jelinek, M. et al. Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings. J Mater Sci: Mater Med 28, 17 (2017). https://doi.org/10.1007/s10856-016-5830-2

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