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Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications

  • Biomaterials Synthesis and Characterization
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Abstract

Chitin biopolymer production and its by-product chitosan show great potential. These biomaterials have great applicability in various fields because they are non-toxic, biodegradable, biocompatible, and have antimicrobial effects. The most common source of chitin and chitosan is the crustaceous shell; however, mushrooms are an alternative source for isolating these biopolymers because their cellular wall has a high content of chitin, which may be transformed into chitosan through a deacetylation reaction. The main objective of this research was to obtain chitosan through the deacetylation of chitin isolated from the Ganoderma lucidum basidiomycetes mushroom, which is obtained through biotechnological culture. The material characterization was performed using X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and an evaluation of cytotoxicity comparing the results obtained with results for commercial chitosan. Protocol results showed that chitosan obtained from this mushroom had a significant similitude with commercial chitosan, yet the one obtained using P2 protocol was the one that rendered the best results: including diffractogram peaks, characteristic infrared analysis bands, and an 80.29 % degree of deacetylation. Cytotoxicity in vitro testing showed that the material was non-toxic; furthermore, it rendered very promising information regarding the evaluation of future applications of this biomaterial in the field of biomedicine.

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Acknowledgments

The authors of this study express their gratitude to both Universidad de Antioquia CODI for its funding of this research project and to the Biotechnology and Biomaterial Research Groups.

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Authors and Affiliations

  1. Biomaterials Research Group, Bioengineering Program, Engineering Faculty, University of Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia

    Natali Mesa Ospina, Diana Marcela Escobar Sierra & Claudia Patricia Ossa Orozco

  2. Biotechnology Research Group, Biology Institute, University of Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia

    Sandra Patricia Ospina Alvarez, Diego Fernando Rojas Vahos & Paola Andrea Zapata Ocampo

Authors
  1. Natali Mesa Ospina
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  2. Sandra Patricia Ospina Alvarez
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  3. Diana Marcela Escobar Sierra
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  4. Diego Fernando Rojas Vahos
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  5. Paola Andrea Zapata Ocampo
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  6. Claudia Patricia Ossa Orozco
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Correspondence to Claudia Patricia Ossa Orozco.

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Mesa Ospina, N., Ospina Alvarez, S.P., Escobar Sierra, D.M. et al. Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications. J Mater Sci: Mater Med 26, 135 (2015). https://doi.org/10.1007/s10856-015-5461-z

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  • DOI: https://doi.org/10.1007/s10856-015-5461-z

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