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The effect of rhBMP-2 and PRP delivery by biodegradable β-tricalcium phosphate scaffolds on new bone formation in a non-through rabbit cranial defect model

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Abstract

This study evaluated whether the combination of biodegradable β-tricalcium phosphate (β-TCP) scaffolds with recombinant human bone morphogenetic protein-2 (rhBMP-2) or platelet-rich plasma (PRP) could accelerate bone formation and increase bone height using a rabbit non-through cranial bone defect model. Four non-through cylindrical bone defects with a diameter of 8-mm were surgically created on the cranium of rabbits. β-TCP scaffolds in the presence and absence of impregnated rhBMP-2 or PRP were placed into the defects. At 8 and 16 weeks after implantation, samples were dissected and fixed for analysis by microcomputed tomography and histology. Only defects with rhBMP-2 impregnated β-TCP scaffolds showed significantly enhanced bone formation compared to non-impregnated β-TCP scaffolds (P < 0.05). Although new bone was higher than adjacent bone at 8 weeks after implantation, vertical bone augmentation was not observed at 16 weeks after implantation, probably due to scaffold resorption occurring concurrently with new bone formation.

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Acknowledgments

We would like to acknowledge the support of the Chonnam National University Hospital Research Institute of Clinical Medicine (Gwangju, Republic of Korea), the National Institutes of Health (NIH, United States), and the Department of Defense (DOD, United States) in the form of Research Grants CRI 11061-1, NIH AR057837 (NIAMS), NIH DE021468 (NIDCR), DOD W81XWH-10-1-0966 (PRORP), DOD W81XWH-10-200-10 (Airlift Research Foundation) and DOD W81XWH-11-2-0168-P4 (Alliance of NanoHealth).

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Authors and Affiliations

  1. Department of Prosthodontics, Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju, 500-757, South Korea

    Hyun-Pil Lim, Kwi-Dug Yun, Taek-Hue Choi & Sang-Won Park

  2. Department of Orthopedic Surgery, Stanford University, 300 Pasteur Drive, Edwards, R155, Stanford, CA, 94305, USA

    Angel E. Mercado-Pagan, Julius Bishop, William Maloney & Yunzhi Peter Yang

  3. College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea

    Seong-Soo Kang

  4. Department of Pharmacology and Dental Therapeutics, Dental Science Research Institute and BK21 Project, School of Dentistry, Chonnam National University, Gwangju, South Korea

    Jeong-Tae Koh

  5. Department of Materials Science and Engineering, Research Institute for Functional Surface Engineering, Chonnam National University, Gwangju, South Korea

    Kwang-Min Lee

  6. Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA

    Yunzhi Peter Yang

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  1. Hyun-Pil Lim
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  2. Angel E. Mercado-Pagan
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  3. Kwi-Dug Yun
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  6. Julius Bishop
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  7. Jeong-Tae Koh
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  8. William Maloney
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Correspondence to Yunzhi Peter Yang or Sang-Won Park.

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Lim, HP., Mercado-Pagan, A.E., Yun, KD. et al. The effect of rhBMP-2 and PRP delivery by biodegradable β-tricalcium phosphate scaffolds on new bone formation in a non-through rabbit cranial defect model. J Mater Sci: Mater Med 24, 1895–1903 (2013). https://doi.org/10.1007/s10856-013-4939-9

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  • DOI: https://doi.org/10.1007/s10856-013-4939-9

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