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Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: a gene expression study using real-time RT-PCR

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Abstract

Biomaterial surface properties, via alterations in the adsorbed protein layer, and the presence of specific functional groups can influence integrin binding specificity, thereby modulating cell adhesion and differentiation processes. The adsorption of fibronectin, a protein directly involved in osteoblast adhesion to the extracellular matrix, has been related to different physical and chemical properties of biomaterial surfaces. This study used blasting particles of different sizes and chemical compositions to evaluate the response of MG63 osteoblast-like cells on smooth and blasted titanium surfaces, with and without fibronectin coatings, by means of real-time reverse transcription-polymerase chain reaction (qRT-PCR) assays. This response included (a) expression of the α5, αv and α3 integrin subunits, which can bind to fibronectin through the RGD binding site, and (b) expression of alkaline phosphatase (ALP) and osteocalcin (OC) as cell-differentiation markers. ALP activity and synthesis of OC were also tested. Cells on SiC-blasted Ti surfaces expressed higher amounts of the α5 mRNA gene than cells on Al2O3-blasted Ti surfaces. This may be related to the fact that SiC-blasted surfaces adsorbed higher amounts of fibronectin due to their higher surface free energy and therefore provided a higher number of specific cell-binding sites. Fn-coated Ti surfaces decreased α5 mRNA gene expression, by favoring the formation of other integrins involved in adhesion over α5β1. The changes in α5 mRNA expression induced by the presence of fibronectin coatings may moreover influence the osteoblast differentiation pathway, as fibronectin coatings on Ti surfaces also decreased both ALP mRNA expression and ALP activity after 14 and 21 days of cell culture.

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Acknowledgments

The authors would like to acknowledge the Spanish Interministerial Commission for Science and Technology (CICYT) for financial support under the MAT2003-08165 project. They would also like to thank Klockner S. L. for technical help in sample preparation and MPA, S. L. (Materias Primas Abrasivas) for providing the blasting particles and blasting machine. M. Pegueroles would like to thank the Universitat Politècnica de Catalunya (UPC) for grant funding to complete her PhD thesis.

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Author notes

  1. G. Pavon

    Present address: Laboratoire de Bio-ingénierie de Polymères Cardiovasculaires—INSERM U698, Institut Galilée, Bâtiment E, Université Paris 13 Nord, 99 Avenue J.B. Clément, 93430, Villetaneuse, France

  2. C. Aparicio

    Present address: Department of Restorative Sciences, Minnesota Dental Research Center for Biomaterials and Biomechanics (MDRCBB), School of Dentistry, University of Minnesota, 16-212 Moos Tower, 515 Delaware St. S.E., Minneapolis, MN, 55455, USA

Authors and Affiliations

  1. Biomaterials, Biomechanics, and Tissue Engineering Group, Department of Material Science and Metallurgy, Universitat Politècnica de Catalunya, Av. Diagonal 647, 08028, Barcelona, Spain

    M. Pegueroles, E. Engel, F. J. Gil, J. A. Planell & C. Aparicio

  2. Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 13, 08028, Barcelona, Spain

    A. Aguirre, E. Engel & J. A. Planell

  3. Laboratoire des Biomatériaux et Polymères de Spécialité—UMR CNRS 7052, Institut Galilée, 99 Avenue J.B. Clément, 93430, Villetaneuse, France

    G. Pavon & V. Migonney

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Pegueroles, M., Aguirre, A., Engel, E. et al. Effect of blasting treatment and Fn coating on MG63 adhesion and differentiation on titanium: a gene expression study using real-time RT-PCR. J Mater Sci: Mater Med 22, 617–627 (2011). https://doi.org/10.1007/s10856-011-4229-3

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  • DOI: https://doi.org/10.1007/s10856-011-4229-3

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