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Preparation and antibacterial characteristic of water-insoluble antibacterial material QPEI/SiO2

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Abstract

Polyethyleneimine (PEI) was grafted onto micron-sized silica gel particles via the coupling action of γ-chloropropyl trimethoxy silane, and grafting particles PEI/SiO2 was prepared. Then, polymeric reactions of two steps, tertiary amination reaction and quaterisation, were conducted for the grafted PEI. After quaterisation of the grafted polyethyleneimine, a composite particle, QPEI/SiO2, was obtained. QPEI/SiO2 is a water-insoluble antibacterial material. In this work, the antibacterial characteristic of QPEI/SiO2 was mainly investigated using Escherichia coli (E. coli) as a disease-leading bacterium and adopting a colony count method. The effects of quaterisation degree of PEI and pH of the medium on the antibacterial ability of QPEI/SiO2 were examined. The antibacterial mechanism of QPEI/SiO2 was explored profoundly by using two enzyme activity determination methods, β-d-galactosidase activity determination and TTC-dehydrogenase activity determination. The experimental results indicates that the water-insoluble antibacterial material QPEI/SiO2 possesses strong antibacterial ability, for the bacterial suspension with a concentration of 109 CFU/ml, the antibacterial ratio of QPEI/SiO2 can reach about 100% only with a dosage of 15 g/l and only for a contact time of 10 min. The main factors influencing the antibacterial ratio of QPEI/SiO2 are the quaterisation degree of the grafted PEI and pH value of the medium. QPEI/SiO2 with higher quaterisation degree has stronger antibacterial ability. In a certain range of pH value, the higher the pH value of the medium is, the stronger the antibacterial ability of QPEI/SiO2 is. Enzyme activity determination results show that the antibacterial mechanism of the water-insoluble antibacterial material QPEI/SiO2 is based on a sterilization process and not only is a bacteriostasis action.

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Authors and Affiliations

  1. Department of Chemical Engineering, North University of China, Taiyuan, 030051, P.R. China

    Baojiao Gao, Xin Zhang & Jian Wang

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  1. Baojiao Gao
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  2. Xin Zhang
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  3. Jian Wang
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Correspondence to Baojiao Gao.

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Gao, B., Zhang, X. & Wang, J. Preparation and antibacterial characteristic of water-insoluble antibacterial material QPEI/SiO2 . J Mater Sci: Mater Med 19, 3021–3028 (2008). https://doi.org/10.1007/s10856-008-3428-z

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  • DOI: https://doi.org/10.1007/s10856-008-3428-z

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