Abstract
Electrospinning is regarded as a facile tool to generate biomaterials into a nanofibrous structure. Herein a nanofibrous web constituted of collagen and hydroxyapatite (HA) was produced from their co-precipitated nanocomposite solution by using the electrospinning method. The co-precipitated sol was freeze-dried and the dried product was dissolved in an organic solvent for the electrospinning. The electrospun web showed a well-developed nanofibrous structure with HA contents of up to 20 wt%. The internal structure of the collagen–20 wt%HA nanofiber revealed highly elongated apatite nanocrystallines precipitated within the collagen matrix. However, above the HA content of 30 wt% the nanofibrous structure could not be preserved due to the formation of beads. The MC3T3-E1 osteoblastic cells were shown to adhere and grow actively on the collagen–HA nanofibrous web. The alkaline phosphatase (ALP) activity expressed by the cells on the collagen–20 wt%HA nanofiber was lower at day 7, but was higher at day 14 than that on the pure collagen nanofiber. Based on the study, the newly-developed collagen–HA nanofiber may be useful as a cell supporting substrate in bone regeneration area.
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References
L.L. Hench, J. Am. Ceram. Soc. 81, 1785 (1998)
P.W. Brown, B. Constantz, Hydroxyapatite and Related Materials. (CRC Press, Boca Raton, 1994)
H.W. Kim, H.E. Kim, V. Salih, Biomaterials 26, 5221 (2005)
A.L. Boskey, Calcif. Tissue Int. 63, 179 (1998)
W.F. Newman, M.W. Newman, The Chemical Dynamics of Bone Mineral. (University of Chicago Press, Chicago, 1958)
J.H. Song, H.W. Kim, H.E. Kim, J Biomed Mater Res B 83B, 248 (2007)
B.H. Yoon, H.W. Kim, S.H. Lee, C.J. Bae, Y.H. Koh, Y.M. Kong, H.W. Kim, Biomaterials 26, 2957 (2005)
A. Tampieri, G. Celotti, E. Landi, M. Sandri, N. Roveri, G. Falini, J. Biomed. Mater. Res. A. 67A, 618 (2003)
D. Lickorish, J.A.M. Ramshaw, A.J. Werkmeister, V. Glattauer, C.R. Howlett, J. Biomed. Mater. Res. A. 68A, 19 (2004)
Y. Dzenis, Science. 304, 1917 (2004)
D. Li, Y. Xia, Adv. Mater. 16(14), 1151 (2004)
H.W. Kim, H.S. Yu, H.H. Lee, J. Biomed. Mater. Res. A. (2008). doi:10.1002/jbm.a.31677
W.J. Li, R. Tuli, X. Huang, P. Laquerriere, R.S. Tuan, Biomaterials 26, 5158 (2005)
H. Yoshimoto, Y.M. Shin, H. Terai, J.P. Vacanti, Biomaterials 24, 2077 (2003)
Z.M. Huang, Y.Z. Zhang, M. Kotaki, S. Ramakrishna, Comp. Sci. Technol. 63, 2223 (2003)
J.H. Song, H.E. Kim, H.W. Kim, J. Mater. Sci. Mater. Med. 19, 95 (2008)
K. Fujihara, M. Kotaki, S. Ramakrishna, Biomaterials 26, 4139 (2006)
H.W. Kim, J.H. Song, H.E. Kim, Adv. Fun. Mater. 15, 1988 (2005)
H.W. Kim, H.H. Lee, J.C. Knowles, J. Biomed. Mater. Res. A. 79A, 643 (2006)
E.D. Boland, J.A. Matthews, K.J. Pawlowski, D.G. Sompson, G.E. Wnek, L. Gary, Front Biosci. 9, 1432 (2004)
J.A. Matthews, G.E. Wnek, D.G. Simpson, G.L. Bowlin, Biomacromol. 3, 232 (2002)
F. Ko, Y. Gogotsi, A. Ali, N. Naguib, H. Ye, G. Yang, C. Li, P. Willis, Adv. Mater. 15, 1161 (2005)
M.M. Stevens, J.H. George, Science. 310, 1135 (2005)
K.M. Woo, J.H. Jun, V.J. Chen, J. Seo, J.H. Baek, H.M. Rhoo, G.S. Kim, M.J. Somerman, P.X. Ma, Biomaterials 28, 335 (2007)
I.K. Kwon, S. Kidoaki, T. Matsuda, Biomaterials. 26, 3929 (2005)
S.H. Park, T.I. Kim, Y. Ku, C.P. Chung, S.B. Han, J.H. Yu, S.P. Lee, H.W. Kim, H.H. Lee, J. Ceram. Soc. Jp. 116, 31 (2008)
J.A.R. Gordon, C.E. Tye, A.V. Sampaio, T.M. Underhill, G.K. Hunter, H.A. Goldberg, Bone. 41, 462 (2007)
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Song, JH., Kim, HE. & Kim, HW. Electrospun fibrous web of collagen–apatite precipitated nanocomposite for bone regeneration. J Mater Sci: Mater Med 19, 2925–2932 (2008). https://doi.org/10.1007/s10856-008-3420-7
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DOI: https://doi.org/10.1007/s10856-008-3420-7