Abstract
In this study, gelatin, was successfully electrospun from a newly developed water-based co-solvent composed of ethyl acetate and acetic acid in water. Since natural polymers including gelatin exhibit limited solubility in water, toxic or highly acidic solvents are normally used to dissolve them for electrospinning. Instead of using those solvents, we used ethyl acetate in concert with acetic acid in water, and investigated the beneficial effect of its use in terms of the spinnability of the nanofiber and the acidity of the solvent. The replacement of acetic acid with ethyl acetate was observed to improve the spinnability of the nanofiber by reducing the surface tension of the solution as well as to increase the pH of the solvent significantly. The optimal composition of the co-solvent was found to correspond to a ratio of ethyl acetate to acetic acid of 2:3. Under this solvent condition, the gelatin could be dissolved at concentrations of up to ∼11 wt% and electrospun successfully to produce nanofibers with various diameters (47–145 nm on average) depending on the gelatin concentration. The water-based co-solvent method proposed herein may be useful for generating other nanofibrous natural polymers as well as being applicable in delivery systems for bioactive molecules within the nanofiber matrices.
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R LANGER and D. A. TIRRELL, Nature 428 (2004) 487
P. Y. W DANKERS, M. C. HARMSEN, L. A. BROUWER, M. J. A Van LUYN and E. W. MEIJER, Nature Mater. 4 (2005) 568
Z. M. HUANG, Y. Z. ZHANG, M. KOTAKI and S. RAMARKRISHINA, Compos. Sci. Technol. 63 (2005) 2223
H. LIU and Y. L. HSIEH, J. Polym. Sci. 40 (2002) 2119
Y. ZHANG, H. OUYANG, C. T. LIM, S. RAMARKRISHINA and Z. M. HUANG, J. Biomed. Mater. Res. Part B: Appl. Biomater. 72B (2005) 156
H. W. KIM, J. H. SONG and H. E. KIM, Adv. Funct. Mater. 15 (2005) 1988
M. LI, M. J. MONDRINOS, M. R. GANDHI, F. K. KO, A. S. WEISS and P. I. LELKES, Biomaterials 26 (2005) 5999
G. E. WNEK, M. E. CARR, D. G. SIMPSON and G. L. BOWLIN, Nano Lett. 3 (2003) 213
K. OHKAWA, D. CHA, H. KIM, A. NISHIDA and H. YAMAMOTO, Macromol. Rapid Commun. 25 (2004) 1600
J. A. MATTHEWS, G. E. WNEK, D. G. SIMPSON and G. L. BOWLIN, Biomacromolecules 3 (2002) 232
B. DUAN, C. DONG, X. YUAN and K. YAO, J. Biomater. Sci. Polymer Edn. 15 (2004) 797
L. HUANG, K. NAGAPUDI, R. P. APKARIAN and E. L. CHAIKOF, J. Biomater. Sci. Polymer Edn. 12 (2001) 979
X. GENG, O. H. KWON and J. JANG, Biomaterials, 26 (2005) 5427
C. S. KI, D. H. BAEK, K. D. GANG, K. H. LEE, I. C. UM and Y. H. PARK, Polymer 46 (2005) 5094
C. BURGER, B. S. HSIAO and B. CHU, Ann Rev Mater Res 36 (2006) 333
M. D. MISAK, J. Colloid Interf. Sci. 27 (1968) 141
M. M. HOHMAN, M. SHIN, G. RUTLEDGE and M. P. BRENNER, Phys. Fluids 13 (2001) 2221
X. WANG, I. C. UM, D. FANG, A. OKAMOTO, B. S. HSIAO and B. CHU, Polymer 49 (2005) 4853
P. C. HEIMENZ and R. RAJAGOPALAN, in “Principles of Colloid and Surface Chemistry”, 3rd ed. (M. Dekker, New York, 1997)
FARBWERKE HOECHST AG, Hoechst Solvents. Frankfurt (M), Germany (1969)
Ş. A. ÇETINUS and H. N. ÖZTOP, Enzyme Microb. Technol. 32 (2003) 889
A. N. FRAGA and R. J. J. WILLIAMS, Polymer 26 (1985) 113
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Song, JH., Kim, HE. & Kim, HW. Production of electrospun gelatin nanofiber by water-based co-solvent approach. J Mater Sci: Mater Med 19, 95–102 (2008). https://doi.org/10.1007/s10856-007-3169-4
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DOI: https://doi.org/10.1007/s10856-007-3169-4