Skip to main content
SpringerLink
Log in

Competitive protein adsorption to polymer surfaces from human serum

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Surface modification by “soft” plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before. Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. B. D. RATNER, A. S. HOFFMAN, F. J. SCHOEN and J. E. LEMONS, Biomaterials Science. An Introduction to Materials in Medicine. 2nd ed. (Elsevier Academic Press, San Diego, USA, 2004)

    Google Scholar 

  2. A. S. HOFFMAN, J Biomater Sci-Polymer Ed 10(10) (1999) 1011

    Article  CAS  Google Scholar 

  3. D. G. CASTNER and B. D. RATNER, Surf Sci 500 (2002) 28

    Article  CAS  Google Scholar 

  4. Z. ADEMOVIC, J. WEI, B. WINTHER-JENSEN, X. L. HOU and P. KINGSHOTT, Plasma Process Polym 2 (2005) 53

    Article  CAS  Google Scholar 

  5. H. NOH and E. A. VOGLER, Biomaterials 28 (2007) 405

    Article  CAS  Google Scholar 

  6. B. LASSEN and M. MALMSTEN, J Colloid Interface Sci 180 (1996) 339

    Article  CAS  Google Scholar 

  7. M. S. WAGNER, M. SHEN, T. A. HORBETT and D. G. CASTNER, J Biomed Mater Res Part A 64 (2002) 1

    Google Scholar 

  8. C. A. TESKE, M. SCHROEDER, R. SIMON and J. HUBBUCH, J Phys Chem B 109 (2005) 13811

    Article  CAS  Google Scholar 

  9. E. F. IRWIN, J. E. HO, S. R. KANE and K. E. HEALY, Langmuir 21 (2005) 5529

    Article  CAS  Google Scholar 

  10. R. J. GREEN, M. C. DAVIES, C. J. ROBERTS and S. J. B. TENDLER, Biomaterials 20 (1999) 385

    Article  CAS  Google Scholar 

  11. S. N. RODRIGUES, I. C. GONCALVES, M. C. L. MARTINS, M. A. BARBOSA and B. D. RATNER, Biomaterials 27 (2006) 5357

    Article  CAS  Google Scholar 

  12. M. S. WAGNER, T. A. HORBETT and D. G. CASTNER, Biomaterials 24 (2003) 1897

    Article  CAS  Google Scholar 

  13. A. KRISHNAN, C. A. SIEDLECKI and E. A. VOGLER, Langmuir 20 (2004) 5071

    Article  CAS  Google Scholar 

  14. S. Y. JUNG, S. M. LIM, F. ALBERTORIO, G. KIM, M. C. GURAU, R. D. YANG, M. A. HOLDEN and P. S. CREMER, J Am Chem Soc 125 (2003) 12782

    Article  CAS  Google Scholar 

  15. A. KRISHNAN, P. CHA, Y. H. LIU, D. ALLARA and E. A. VOLGER, Biomaterials 27 (2006) 3187

    Article  CAS  Google Scholar 

  16. T. ARNEBRANT and T. NYLANDER, J Colloid Interface Sci 111(2) (1986) 529

    Article  CAS  Google Scholar 

  17. L. VROMAN and A. L. ADAMS, Surf Sci 16 (1969) 438

    Article  CAS  Google Scholar 

  18. L. VROMAN, A. L. ADAMS and M. KLINGS, Fed Am Soc Exp Biol Fed Proc 30 (1971) 1494

    CAS  Google Scholar 

  19. C. F. LU, A. NADARAJAH and K. K. CHITTUR, J Colloid Interface Sci 168 (1994) 152

    Article  CAS  Google Scholar 

  20. M. MALMSTEN, J Colloid Interface Sci 207 (1998) 186

    Article  CAS  Google Scholar 

  21. F. FANG and I. SZLEIFER, Biophys J 80 (2001) 2568

    CAS  Google Scholar 

  22. Z. ADEMOVIC, B. HOLST, R. A. KAHN, I. JØRRING, T. BREVIG, J. WEI, X. L. HOU, B. WINTHER-JENSEN and P. KINGSHOTT, J Mater Sci— Mat Med 17 (2006) 203

    Article  CAS  Google Scholar 

  23. H. YASUDA, Luminous Chemical Vapor Deposition and Interface Engineering (Marcel Dekker, New York, 2005) p. 119

  24. M. HOLMBERG, K. B. STIBIUS, S. NDONI, N. B. LARSEN, P. KINGSHOTT and X. L. HOU, Anal Biochem 361 (2007) 120

    Article  CAS  Google Scholar 

  25. G. H. HINKLE, B. S. LOESCH, R. NINES, L. DEPALATIS and D. P. HOUCHENS, J Labelled Comp Radiopharm XLI (1998) 423

    Article  Google Scholar 

  26. G. BEAMSON, D. BRIGGS, High Resolution XPS of Organic Polymers—The Scienta ESCA300 Database (Wiley, 1992)

  27. M. VAN DER VEEN, M. C. STUART and W. NORDE, Colloids Surf B: Biointerf 54 (2007) 136

    Article  CAS  Google Scholar 

  28. S. H. MOLLMANN, L. JORGENSEN, J. T. BUKRINSKY, U. ELOFSSON, W. NORDE and S. FROKJÆR, Eur J Pharm Sci 27 (2006) 194

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Polymer Department, Risø National Laboratory, Technical University of Denmark, Building 124, P.O. Box 49, 4000, Roskilde, Denmark

    Maria Holmberg, Karin B. Stibius & Niels B. Larsen

  2. Radiation Research Department, Risø National Laboratory, Technical University of Denmark, 4000, Roskilde, Denmark

    Xiaolin Hou

  3. Faculty of Pharmaceutical Science, University of Copenhagen, 2100, Copenhagen, Denmark

    Karin B. Stibius

Authors
  1. Maria Holmberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin B. Stibius
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Niels B. Larsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaolin Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria Holmberg.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Holmberg, M., Stibius, K.B., Larsen, N.B. et al. Competitive protein adsorption to polymer surfaces from human serum. J Mater Sci: Mater Med 19, 2179–2185 (2008). https://doi.org/10.1007/s10856-007-3318-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-007-3318-9

Keywords

Search

Navigation