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The method of surface PEGylation influences leukocyte adhesion and activation

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Abstract

The influence of different surface modifications with poly(ethyleneglycol) (PEG) layers on the adsorption of fibrinogen and the adhesion and activation of macrophage-like human leukocytes was investigated. Poly(ethylene terephthalate) (PET) was modified using pulsed AC plasma polymerization with two types of starting monomers to generate: 1) a reactive acid surface using maleic anhydride (MAH) as monomer, and 2) a PEG-like surface using diethyleneglycol methyl vinyl ether (DEGVE) as monomer. The MAH surface was used as a reactive platform to graft linear chains of non-fouling mPEG via an intermediate layer of poly(ethyleneimine) (PEI) under lower critical solution temperature (LCST) conditions of the mPEG. The DEGVE monomer is used to create PEG-like layers by use of low power plasma conditions. The ability of the surfaces to resist protein adsorption was investigated quantitatively using 125I-radiolabeled human fibrinogen, and the conformation of the adsorbed protein was tested using an anti-fibrinogen monoclonal antibody in an enzyme-linked immunosorbent assay. The results showed that PEGylated surfaces adsorbed significantly less (up to 90% less) fibrinogen, and that unfolding of adsorbed fibrinogen was more pronounced on the linear mPEG layers than on the PEG-like plasma polymer surfaces. Adhesion of in-vitro differentiated macrophage-like U937 cells was reduced on both the PEG-like plasma polymer surfaces and the linear mPEG layers compared to the unmodified PET surface, but cells adhering to the PEG-like plasma polymer surfaces secreted less tumor necrosis factor-α (TNF- α) than cells adhering to the linear mPEG layers. In conclusion, the method for preparing non-fouling surfaces for long-term implanted devices influence surface-induced cellular responses of the host.

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Authors and Affiliations

  1. Danish Polymer Centre, Risø National Laboratory, Frederiksborgvej 399, 4000, Roskilde, Denmark

    Z. Ademovic, J. Wei, B. Winter-Jensen & P. Kingshott

  2. Bioneer A/S, Mammalian Cell Biology, Kogle Alle 2, 2970, Hørsholm, Denmark

    B. Holst, R. A. Kahn, I. Jørring & T. Brevig

  3. Nuclear Research Department, Risø National Laboratory, Frederiksborgvej 399, 4000, Roskilde, Denmark

    X. Hou

Authors
  1. Z. Ademovic
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  2. B. Holst
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  3. R. A. Kahn
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  4. I. Jørring
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  5. T. Brevig
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  6. J. Wei
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  8. B. Winter-Jensen
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  9. P. Kingshott
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Correspondence to P. Kingshott.

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Ademovic, Z., Holst, B., Kahn, R.A. et al. The method of surface PEGylation influences leukocyte adhesion and activation. J Mater Sci: Mater Med 17, 203–211 (2006). https://doi.org/10.1007/s10856-006-7306-2

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  • DOI: https://doi.org/10.1007/s10856-006-7306-2

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