Abstract
Mitoxantrone (DHAQ)-loaded poly (ethylene glycol)-poly (lactic acid-co-glycolic acid) –poly (ethyleneglycol) (PELGE) nanoparticles (NP) were fabricated using an emulsification/solvent evaporation technique. A central composite design (CCD) was applied to evaluate the joint influence of three formulation variables: the amounts of polymer, concentration of the DHAQ, and the ratio of the organic phase (inner-phase) and the aqueous phase (outer-phase). In this study, we optimize the preparation technology on the basis of the single factor evaluation. The optimal conditions for the preparation of DHAQ-loaded nanoparticle were found to be: the concentration of PELGE was 9 mg/mL, the concentration of inner-phase of DHAQ was 27.5 mg/L, and the ratio of inner-phase/outer-phase was 8.5/1. The results showed that CCD is an ideal technique for formulation studies. The entrapment efficiency ratio (ER) was 90% and particle sizes are less than 500 nm. The nanoparticles, as examined by transmission electron microscopy (TEM), have a smooth and spherical surface. The DHAQ could be loaded into PELGE copolymers. In this study, the DHAQ nanoparticle-polymer delivery system was established by using PELGE polymers as carrier material.
Similar content being viewed by others
References
K. AVGOUSTAKIS, A. BELETSI and D. S. ITHAKISSIOS, J. Contr. Rel. 81 (2002) 123.
S. BRANCHU, R. T. FORBES and P. YORK, et al., Pharm. Res. 16 (1999) 702.
M. FORADADA, M. GALLARDO and J. EATELRICH, J. Pharm. Biomed. Anal. 12 (1994) 1495.
A. D. MCLEOD, F. C. LAM, P. K. GUPTA and C. T. HUNG, J. Pharm. Sci. 77 (1988) 704.
J. MOLPECERES, M. GUZMAN and L. BERGES, J. Pharm. Sci. 85 (1996) 206.
H. L. SHENG and J. S. TU, J. Chi. Pharm. Univ. 27 (1996) 211.
S. BOLTON, J. Pharm. Sci. 72 (1983) 362.
B. LU and W. WU, Acta. Pharm. Sin. (In Chinese) 34 (1999) 387.
T. DE BOER, R. BIJMA and K. ENSING, Pharm. Biomed. Anal. 19 (1999) 529.
Y. R. DUAN, Z. R. ZHANG and C. Y. WANG, Key Eng. Mater. 256 (2004) 887.
K. A. ABU-LZZA, L. GARCIA-CONTRERAS and D. R. LU, J. Pharm. Sci. 85 (1996) 144.
Z. R. ZHANG and J. X. WANG, Acta. Pharm. Sin. (In Chinese) 36 (2001) 456.
Y. R. DUAN, W. S. LIU and Z. R. ZHANG, Key Eng. Mater. 288 (2005) 163.
Y. I. JEONG, C. S. CHO and J. W. NAH, et al., J. Appl. Polym. Sci. 80 (2001) 2228.
R. GREF, Y. MINAMITAKE and, R. LANGER, et al., Science 263 (1994) 1600.
H. FESSI, F. PUISIEUX and S. BENITA, et al., Int. J. Pharm. 55 (1989) R1.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Duan, Y., Xu, S., Wang, Q. et al. Optimization of preparation of DHAQ-loaded PEG-PLGA-PEG nonaparticles using central composite design. J Mater Sci: Mater Med 17, 559–563 (2006). https://doi.org/10.1007/s10856-006-8940-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10856-006-8940-4