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Phospholipids as implant coatings

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Abstract

Bio-interfaces such as bio-membranes are of outmost importance for a variety of live processes. Among them are cell-interactions which take place in, on or through cell membranes. Therefore we propose to cover metallic surfaces with phospholipids to facilitate cell-material interaction. Four lipids, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2- oleoyl-sn-glycero-3-[phospho-L-serine] (POPS) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG), were applied to four metallic growth substrates with different surface structure, roughness and porosity. The interaction of the osteosarcoma cell line MG-63 was investigated in terms of cell adhesion and viability (MTT (methylthiazolyldiphenyl-tetrazolium bromide) assay). While POPS in general had a negative influence, the most suitable combination in terms of viability per adherent MG-63 is the coating of porous Ti6Al4V material with the phospholipids POPE or POPC. The analysis of viability of mouse macrophages RAW 264.7 and their tumor necrosis factor α (TNF-α) release showed that the adhesion and viability is worst on POPS while the TNF-α release was highest. To elucidate the potential of phospholipids to prevent or support bacterial growth, the bacterial number of Gram positive and Gram negative bacteria was investigated. For lipid concentrations higher than 1 mM in solution a growth stimulating effect independent of the lipid type was detected. On a lipid coated surface the number of bacteria was reduced by 81%, 74% and 51% for POPC, POPG and POPE.

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References

  1. C. KUNG, Nature. 436 (2005) 647.

    Article  CAS  Google Scholar 

  2. P. J. BOOTH, Curr. Opin. Struct. Biol. 15 (2005) 435.

    Article  CAS  Google Scholar 

  3. H. SPRONG, P. VAN DER SLUIJS and G. VAN MEER, Nat. Rev. Mol. Cell. Biol. 2 (2001) 504.

    Article  CAS  Google Scholar 

  4. H. HEERKLOTZ, Biophys. J. 83 (2002) 2693.

    Article  CAS  Google Scholar 

  5. K. SIMONS and E. IKONEN, Nature. 387 (1997) 569.

    Article  CAS  Google Scholar 

  6. F. ALLEMANN, S. MIZUNO, K. EID, K. E. YATES, D. ZALESKE and J. GLOWACKI, J. Biomed. Mater. Res. 55 (2001) 13.

    Article  CAS  Google Scholar 

  7. B. D. BOYAN, T. W. HUMMERT, D. D. DEAN and Z. SCHWARTZ, Biomaterials 17 (1996) 137.

    Article  CAS  Google Scholar 

  8. E. P. BRIGGS, A. R. WALPOLE, P. R. WILSHAW, M. KARLSSON and E. PALSGARD, J. Mater. Sci. Mater. Med. 15 (2004) 1021.

    Article  CAS  Google Scholar 

  9. N. MORITZ, S. ROSSI, E. VEDEL, T. TIRRI, H. YLANEN, H. ARO and T. NARHI, J. Mater. Sci. Mater. Med. 15 (2004) 795.

    Article  CAS  Google Scholar 

  10. M. BRIZUELA, A. GARCIA-LUIS, J. L. VIVIENTE, I. BRACERAS and J. I. ONATE, J. Mater. Sci. Mater. Med. 13 (2002) 1129.

    Article  CAS  Google Scholar 

  11. J. S. CHEN, H. Y. JUANG and M. H. HON, J. Mater. Sci. Mater. Med. 9 (1998) 297.

    Article  CAS  Google Scholar 

  12. J. ANDERSSON, K. N. EKDAHL, J. D. LAMBRIS and B. NILSSON, Biomaterials 26 (2005) 1477.

    Article  CAS  Google Scholar 

  13. M. E. ROY and S. K. NISHIMOTO, Bone. 31 (2002) 296.

    Article  CAS  Google Scholar 

  14. T. G. KOOTEN, C. L. KLEIN, H. KOHLER, C. J. KIRKPATRICK, D. F. WILLIAMS and R. ELOY, J. Mater. Sci. Mater. Med. 8 (1997) 835.

    Article  Google Scholar 

  15. M. OTTO, B. WAHN and C. J. KIRKPATRICK, J. Mater. Sci. Mater. Med. 14 (2003) 263.

    Article  CAS  Google Scholar 

  16. S. ROESSLER, R. BORN, D. SCHARNWEBER, H. WORCH, A. SEWING and M. DARD, J. Mater. Sci. Mater. Med. 12 (2001) 871.

    Article  CAS  Google Scholar 

  17. M. C. SIEBERS, P. J. TER BRUGGE, X. F. WALBOOMERS and J. A. JANSEN, Biomaterials 26 (2005) 137.

    Article  CAS  Google Scholar 

  18. R. P. RICHTER, J. L. K. KIM and A. BRISSON, Mater. Today. 6 (2003) 32.

    Article  CAS  Google Scholar 

  19. M. TROJANOWICZ, Fresenius J. Anal. Chem. 371 (2001) 246.

    Article  CAS  Google Scholar 

  20. K. ISHIHARA, H. OSHIDA, Y. ENDO, T. UEDA, A. WATANABE and N. NAKABAYASHI, J. Biomed. Mater. Res. 26 (1992) 1543.

    Article  CAS  Google Scholar 

  21. P. D. MOREIRA, L., P. R. MARRECO, A. M. MORAES, M. L. WADA and S. C. GENARI, J. Biomed. Mater. Res. 69B (2004) 38.

    Article  Google Scholar 

  22. R. F. ZWAAL, P. COMFURIUS and E. M. BEVERS, Cell. Mol. Life. Sci. 62 (2005) 971.

    Article  CAS  Google Scholar 

  23. R. WILLUMEIT, H. KAMUSEWITZ, M. SCHOSSIG, J. SCHRÖDER and H. CLEMENS, Mater. Res. Soc. Symposium Proc.. 734 (2003) B8.6.1.

  24. D. S. SCHWARTZ, D. S. SHIH, R. J. LEDERICH, R. L. MARTIN and D. A. DENSER, Mater. Res. Soc. Symposium Proc. 521 (1998) 225.

  25. A. POLTORAK, X. HE, I. SMIRNOVA, M. Y. LIU, C. VAN HUFFEL, X. DU, D. BIRDWELL, E. ALEJOS, M. SILVA, C. GALANOS, M. FREUDENBERG, P. RICCIARDI-CASTAGNOLI, B. LAYTON and B. BEUTLER, Science 282 (1998) 2085.

    Article  CAS  Google Scholar 

  26. R. WILLUMEIT, F. FEYERABEND, H. KAMUSEWITZ, M. SCHOSSIG and H. CLEMENS, Matwiss Werkst. 34 (2003) 1084.

    Article  CAS  Google Scholar 

  27. J. A. HAYWARD and D. CHAPMAN, Biomaterials 5 (1984) 135.

    Article  CAS  Google Scholar 

  28. J. T. ELLIOTT, A. TONA, J. T. WOODWARD, C. W. MEUSE, H. M. ELGENDY and A. L. PLANT, IEE Proc. Nanobiotechnol. 151 (2004) 75.

    Article  CAS  Google Scholar 

  29. R. WILLUMEIT, M. KUMPUGDEE, S. S. FUNARI, K. LOHNER, B. P. NAVAS, K. BRANDENBURG, S. LINSER and J. ANDRA, Biochim. Biophys. Acta. 1669 (2005) 125.

    Article  CAS  Google Scholar 

  30. J. T. GROVES, L. K. MAHAL and C. R. BERTOZZI, Langmuir 17 (2001) 5129.

    Article  CAS  Google Scholar 

  31. R. E. WUTHIER, J. Lipid. Res. 9 (1968) 68.

    CAS  Google Scholar 

  32. R. E. WUTHIER, Fed. Proc. 35 (1976) 117.

    CAS  Google Scholar 

  33. J. H. KUO, M. S. JAN, J. JENG and H. W. CHIU, J Control Release. 108 (2005) 442.

    Article  CAS  Google Scholar 

  34. R. A. SCHLEGEL and P. WILLIAMSON, Cell. Death. Differ. 8 (2001) 551.

    Article  CAS  Google Scholar 

  35. R. A. SCHLEGEL, M. K. CALLAHAN and P. WILLIAMSON, Ann NY Acad. Sci. 926 (2000) 217.

    Article  CAS  Google Scholar 

  36. E. M. BEVERS, P. COMFURIUS, D. W. DEKKERS, M. HARMSMA and R. F. ZWAAL, Lupus. 7 (Suppl 2) (1998) S126.

  37. E. M. BEVERS, P. COMFURIUS, D. W. DEKKERS, M. HARMSMA and R. F. ZWAAL, Bio. Chem. 379 (1998) 973.

    CAS  Google Scholar 

  38. E. M. BEVERS, P. COMFURIUS, D. W. DEKKERS and R. F. ZWAAL, Biochim. Biophys. Acta. 1439 (1999) 317.

    CAS  Google Scholar 

  39. V. A. FADOK, D. L. BRATTON and P. M. HENSON, J. Clin. Invest. 108 (2001) 957.

    Article  CAS  Google Scholar 

  40. F. SUSKA, C. GRETZER, M. ESPOSITO, P. TENGVALL and P. THOMSEN, Biomaterials 26 (2005) 5942.

    Article  CAS  Google Scholar 

  41. A. K. REFAI, M. TEXTOR, D. M. BRUNETTE and J. D. WATERFIELD, J. Biomed. Mater. Res. A. 70 (2004) 194.

    Article  CAS  Google Scholar 

  42. E. DE LEEUW, K. TE KAAT, C. MOSER, G. MENESTRINA, R. DEMEL, B. DE KRUIJFF, B. OUDEGA, J. LUIRINK and I. SINNING, Embo. J. 19 (2000) 531.

    Article  CAS  Google Scholar 

  43. D. C. WHITE and A. N. TUCKER, J. Lipid. Res. 10 (1969) 220.

    CAS  Google Scholar 

  44. H. GOLDFINE, J. Lipid. Res. 25 (1984) 1501.

    CAS  Google Scholar 

  45. L. R. BERUBE, M. K. SCHUR, R. K. LATTA, T. HIRAMA, C. R. MCKENZIE and H.C. JARRELL, Microb. Pathog. 26 (1999) 65.

    Article  CAS  Google Scholar 

  46. J. D. PATEL, Y. IWASAKI, K. ISHIHARA and J. M. ANDERSON, J. Biomed. Mater. Res. A. 73 (2005) 359.

    Google Scholar 

  47. Y. WU, B. LAU, S. SMITH, K. TROYAN and D. E. BARNETT FOSTER, Infect. Immun. 72 (2004) 6764.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. GKSS Research Centre, Institute for Materials Research, Max-Planck-Str. 1, D-21502, Geesthacht, Germany

    R. Willumeit, A. Schuster, P. Iliev, S. Linser & F. Feyerabend

  2. Department of Macromolecular Structure Research, GKSS Research Centre, Institute for Materials Research, Max-Planck-Str. 1, D-21502, Geesthacht, Germany

    R. Willumeit & F. Feyerabend

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  1. R. Willumeit
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  2. A. Schuster
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  3. P. Iliev
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  5. F. Feyerabend
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Correspondence to R. Willumeit.

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Willumeit, R., Schuster, A., Iliev, P. et al. Phospholipids as implant coatings. J Mater Sci: Mater Med 18, 367–380 (2007). https://doi.org/10.1007/s10856-006-0702-9

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  • DOI: https://doi.org/10.1007/s10856-006-0702-9

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