Abstract
Scaffolds are an important aspect of the tissue engineering approach to tissue regeneration. This study shows that it is possible to manufacture scaffolds from type I collagen with or without hydroxyapatite (HA) by critical point drying. The mean pore sizes of the scaffolds can be altered from 44 to 135 μm depending on the precise processing conditions. Such pore sizes span the range that is likely to be required for specific cells. The mechanical properties of the scaffolds have been measured and behave as expected of foam structures. The degradation rate of the scaffolds by collagenase is independent of pore size. Dehydrothermal treatment (DHT), a common method of physically crosslinking collagen, was found to denature the collagen at a temperature of 120∘C resulting in a decrease in the scaffold’s resistance to collagenase. Hybrid scaffold structures have also been manufactured, which have the potential to be used in the generation of multi-tissue interfaces. Microchannels are neatly incorporated via an indirect solid freeform fabrication (SFF) process, which could aid in reducing the different constraints commonly observed with other scaffolds.
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References
R. CORTESINI, Transpl. Immunol. 15 (2005) 81.
F. R. ROSE and R. O. C. OREFFO, Biochem. Biophys. Res. Commun. 292 (2002) 1.
S. DEDHAR et al., J. Cell Biol. 104 (1987) 585.
W. D. STAATZ et al., J. Biol. Chem. 266 (1991) 7363.
G. T. CRAIG et al., Biomaterials 10 (1989) 133.
E. LANDI et al., J. Eur. Ceram. Soc. 23 (2003) 2931.
A. L. BOSKEY, Calcif. Tissue Int. 63 (1998) 179.
D. A. WAHL and J. CZERNUSZKA, Eur. Cell. Mater. J. 11 (2006) 43.
V. KARAGEORGIOU and D. KAPLAN, Biomaterials 26 (2005) 5474.
M. NOMI et al., Mol. Aspects Med. 23 (2002) 463.
E. TSURUGA et al., J. Biochem. 121 (1997) 317.
F. J. O’BRIEN et al., Biomaterials 26 (2005) 433.
E. SACHLOS and J. CZERNUSZKA, Eur. Cell. Mater. J. 5 (2003) 29.
M. LEE et al., Biomaterials 26 (2005) 4281.
H. S. TUAN and D. W. HUTMACHER, Comput. Aided Des. 37 (2005) 1151.
S. J. HOLLISTER et al., Biomaterials 23 (2002) 4095.
B. LEUKERS et al., J. Mater. Sci. Mater. Med. 16 (2005) 1121.
I. MARTIN et al., J. Biomech. In Press (2006).
E. SACHLOS et al., Biomaterials 24 (2003) 1487.
E. SACHLOS et al., Mater. Res. Soc. Symp. Proc. 758 (2003) 187.
K. WEADOCK et al., Biomater. Med. Devices Artif. Organs 11 (1983) 293.
Y. TAKAHASHI and Y. TABATA, J. Biomater. Sci. Polym. Ed. 15 (2004) 41.
K. TUZLAKOGLU et al., J. Mater. Sci. Mater. Med. 16 (2005) 1099.
M. ITOH et al., Biomaterials 25 (2004) 2577.
F. R. ROSE et al., Biomaterials 25 (2004) 5507.
I. V. YANNAS, J. Macromol. Sci. Rev. Macromol. Chem. Phys. C 7 (1972) 49.
I. REHMAN and W. BONFIELD, J. Mater. Sci. Mater. Med. 8 (1997) 1.
G. C. KOUMOULIDIS et al., J. Coll. Interf. Sci. 259 (2003) 254.
A. KOCIALKOWSKI et al., Injury 21 (1990) 142.
B. D. BOYAN et al., Biomaterials 17 (1996) 137.
U. MEYER et al., Eur. Cell. Mater. J. 9 (2005) 39.
E. SACHLOS, D Phil thesis, Oxford University (2004) 77.
M. F. ASHBY and D. R. H. JONES, in “Engineering Materials 2: An Introduction to Microstructures, Processing and Design” (Pergamon Press, 1986) p. 250.
H. SCHOOF et al., J. Crystal Growth 209 (2000) 122.
I. V. YANNAS and A. V. TOBOLSKY, Nature 215 (1967) 509.
P. ANGELE et al., Biomaterials 25 (2004) 2831.
W. BONFIELD, et al., Acta Materialia 46 (1998) 2509.
W. E. HENNINK and C. F. VAN NOSTRUM, Adv. Drug. Deliv. Rev. 54 (2002) 13.
K. S. WEADOCK et al., J. Biomed. Mater. Res. 32 (1996) 221.
M. GEIGER, PhD thesis, Friedrich-Alexander-Universität Erlangen (2001) 84.
S. D. GORHAM, et al., Int. J. Biol. Macromol. 14 (1992) 129.
M.-C. WANG, et al., Biomaterials 15 (1994) 507.
A. BIGI et al., Biomaterials 25 (2004) 5675.
T. J. WESS and J. P. ORGEL, Thermochimica Acta 365 (2000) 119.
P. J. KELLY, J. Bone Joint Surg. Am. 50 (1968) 766.
M. ARTICO et al., Surg. Neurol. 60 (2003) 71.
R. R. BETZ, Orthopedics 25 (2002) S561.
D. SCHAEFER et al., Arthritis Rheum. 46 (2002) 2524.
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Wahl, D.A., Sachlos, E., Liu, C. et al. Controlling the processing of collagen-hydroxyapatite scaffolds for bone tissue engineering. J Mater Sci: Mater Med 18, 201–209 (2007). https://doi.org/10.1007/s10856-006-0682-9
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DOI: https://doi.org/10.1007/s10856-006-0682-9