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The double porogen approach as a new technique for the fabrication of interconnected poly(L-lactic acid) and starch based biodegradable scaffolds

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Abstract

One of the most widely used fabrication methods of three dimensional porous scaffolds involves compression moulding of a polymer salt mixture, followed by salt leaching. However, the scaffolds prepared by this technique have typically limited interconnectivity. In this study, besides salt particles, an additional polymeric porogen, poly(ethylene oxide), PEO, was added to poly(L-lactic acid), PLLA, to enhance the interconnectivity of the scaffolds. Compression moulded specimens were quenched and put into water, where PEO crystallized and phase separated. Following the leaching of PEO fraction, the permeability and interconnectivity among the macropores formed by salt leaching could be observed. The porosities obtained in the prepared scaffolds were between 76 to 86%. Moreover, the highest porosity of 86% was obtained with minimum fraction of total porogen. The water absorption of the porous scaffolds prepared with PEO could vary between 280 to 450% while water uptake of pure PLLA scaffolds was about 93%. The increase of interconnectivity induced by compounding PLLA with PEO could also be obtained in porous PLLA/starch blends and PLLA/hydroxyapatite composites demonstrating the versatility and wide applicability of this preparation protocol. The simplicity of this organic solvent free preparation procedure of three-dimensional porous scaffolds with high interconnectivity and high surface area to volume ratio holds a promise for several tissue engineering applications.

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References

  1. L. E. FREED, D. A. GRANDE, Z. LINGBIN, J. EMMANUAL, J. C. MARQUIS and R. LANGER, J. Biomed. Mater. Res. 28 (1994).

  2. W. L. MURPHY, D. H. KOHN and D. J. MOONEY, J. Biomed. Mater. Res. 50 (2000) 50.

    Article  CAS  Google Scholar 

  3. J. C. MIDDLETON and A. J. TIPTON, Biomaterials 21 (2000) 2335.

    Article  CAS  Google Scholar 

  4. J. M. CHUPA, A. M. FOSTER, S. R. SUMNER, S. V. MADIHALLY and H. W. T. MATTHEW, Biomaterials. 21 (2000) 2315.

    Article  CAS  Google Scholar 

  5. R. L. REIS and J. SAN ROMAN, in “Biodegradable Systems in Tissue Engineering and Regenerative Medicine” (CRC Press, 2005).

  6. A. P. MARQUES, R. L. REIS and J. A. HUNT, Biomaterials 23 (2002) 1471.

    Article  CAS  Google Scholar 

  7. M. E. GOMES, R. L. REIS, A. M. CUNHA, C. A. BLITTERSWIJK and J. D. DE BRUIJN, Biomaterials 22 (2001) 1911.

    Article  CAS  Google Scholar 

  8. A. G. MIKOS, A. J. THORSEN, L. A. CZERWONKA, Y. BAO, R. LANGER, D. N. WINSLOW and J. P. VACANTI, Polymer. 35 (1994) 1068.

    Article  CAS  Google Scholar 

  9. A. P. MARQUES, H. R. CRUZ, O. P. COUTINHO and R. L. REIS, J. Mater. Sci.- Mater. Med. 16 (2005) 833.

    Article  CAS  Google Scholar 

  10. J. LI, X. NI and K. W. LEONG, J. Biomed. Mater. Res. Part A 65 (2003) 196.

    Article  Google Scholar 

  11. J. D. DE BRUIJN, C. A. VAN BLITTERSWIJK and J. E. DAVIES, J. Biomed. Mater. Res. 29 (1995) 89.

    Article  CAS  Google Scholar 

  12. J. A. JUHASZ, S. M. BEST, R. BROOKS, M. KAWASHITA, N. MIYATA, T. KOKUBO, T. NAKAMURA and W. BONFIELD, Biomaterials 25 (2004) 949.

    Article  CAS  Google Scholar 

  13. W. L. MURPHY, M. C. PETERS, D. H. KOHN and D. J. MOONEY, Biomaterials 21 (2000) 2521.

    Article  CAS  Google Scholar 

  14. S. FORMAN, J. KAS, F. FINI, M. STEINBERG and T. RUML, J. Biochem. Mol. Toxicol. 13 (1999) 11.

    Article  CAS  Google Scholar 

  15. K. WHANG, C. H. THOMAS, K. E. HEALY and G. NUBER, Polymer. 36 (1995) 837.

    Article  CAS  Google Scholar 

  16. Q. HOU, D. W. GRIJPMA and J. FEIJEN, Biomaterials 24 (2003) 1937.

    Article  CAS  Google Scholar 

  17. Y. HU, Y. S. HU, V. TOPOLKARAEV, A. HILTNER and E. BAER, Polymer. 44 (2003) 5711.

    Article  CAS  Google Scholar 

  18. T. KOKUBO, H. KUSHITANI, S. SAKKA, T. KITSUGI and T. YAMAMURO, J. Biomed. Mater. Res. 24 (1990) 721.

    Article  CAS  Google Scholar 

  19. D. SHIN, K. SHIN, K. A. AAMER, G. N. TEW, T. P. RUSSELL, J. H. LEE and J. Y. JHO, Macromolecules 38 (2005) 104.

    Article  CAS  Google Scholar 

  20. E. MEAURIO, E. ZUZA and J. R. SARASUA, Macromolecules 38 (2005) 1207.

    Article  CAS  Google Scholar 

  21. J.BRANDRUP and E. H. IMMERGUT, in “Polymer Handbook.” (Wiley, NewYork, 1989) p. 555.

    Google Scholar 

  22. A. J. NIJENHUIS, E. COLSTEE, D. W. GRIJPMA and A. J. PENNINGS, Polymer 37 (1996) 5849.

    Article  CAS  Google Scholar 

  23. R. G. FLEMMING, C. J. MURPHY, G. A. ABRAMS, S. L. GOODMAN and P. F. NEALEY, Biomaterials 20 (1999) 573.

    Article  CAS  Google Scholar 

  24. J. B. RECKNOR, D. S. SAKAGUCHI and S. K. MALLAPRAGADA, Biomaterials 27 (2006) 4098.

    Article  CAS  Google Scholar 

  25. H. TSUJI, R. SMITH, W. BONFIELD and Y. IKADA, J. Appl. Polym. Sci. 75 (2000) 629.

    Article  CAS  Google Scholar 

  26. K. A. SCHULT and D. R. PAUL, J. Polym. Sci, Part B: Polym. Phys. 35 (1997) 655.

    Article  CAS  Google Scholar 

  27. J. REIGNIER and M. A. HUNEAULT, Polymer, 47 (2006) 4703.

    Article  CAS  Google Scholar 

  28. P. LI, D. BAKKER and C. A. VAN BLITTERSWIJK, J. Biomed. Mater. Res. 34 (1997) 79.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, Campus de Gualtar, 4710-057, Braga, Portugal

    S. Ghosh, R. L. Reis & J. F. Mano

  2. IPC- Institute for Polymers and Composites, Campus de Azurém, 4800-058, Guimarães, Portugal

    J. C. Viana

  3. Department of Polymer Engineering, University of Minho, Campus de Azurém, 4800-058, Guimarães, Portugal

    S. Ghosh, J. C. Viana, R. L. Reis & J. F. Mano

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  1. S. Ghosh
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  2. J. C. Viana
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  3. R. L. Reis
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  4. J. F. Mano
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Correspondence to J. F. Mano.

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Ghosh, S., Viana, J.C., Reis, R.L. et al. The double porogen approach as a new technique for the fabrication of interconnected poly(L-lactic acid) and starch based biodegradable scaffolds. J Mater Sci: Mater Med 18, 185–193 (2007). https://doi.org/10.1007/s10856-006-0680-y

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  • DOI: https://doi.org/10.1007/s10856-006-0680-y

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