Skip to main content
SpringerLink
Log in

Preparation of porous biodegradable poly(lactide-co-glycolide)/ hyaluronic acid blend scaffolds: Characterization, in vitro cells culture and degradation behaviors

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

A series of poly(lactide-co-glycolide) (PLGA)/ hyaluronic acid (HA) blend with different HA composition were used to fabricate scaffolds successfully. The pores of the three dimensional scaffold were prepared by particle leaching and freeze drying. The pore size was about 50–200 μ m and the porosity was about 85%. The characterizations of the scaffold, such as mechanical properties, hydrophilicity and surface morphologies were determined. Mouse 3T3 fibroblast was directly seeded on the scaffolds. The cell adhesion efficiency, cell morphology observed by scanning electron microscopy (SEM) and the degradation behavior of the blend scaffold were evaluated. In summary, the results show that the adhesion efficiency of cells on the PLGA/HA blend scaffold is higher than that on the PLGA scaffold. Moreover, the incorporation of HA in PLGA not only helps to increase the cell affinity but also tends to lead the water and nutrient into the scaffold easily.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. Hatefi and B. Amsden, J. Controlled Release 80 (2002) 9.

    Article  CAS  Google Scholar 

  2. G. Winzenburg, C. Schmidt, S. Fuchs and T. Kissel. Advanced Drug delivery reviews 56 (2004) 1453.

    Article  CAS  Google Scholar 

  3. B. Jeong, Y. K. Choi, Y. H. Bae, G. Zenter and S. W. Kim, J. Controlled Release 62 (1999) 109.

    Article  CAS  Google Scholar 

  4. Rajeer A. Jain, Biomaterials 21 (2000) 2475.

    Article  Google Scholar 

  5. G. Cacciola, G. W. M. Peters and F. P. T. Baaijens, J. Biomechanics 33 (2000) 653.

    Article  CAS  Google Scholar 

  6. Y. Noishiki, Mater. Sci. Eng. C 6 (1998) 227.

    Article  Google Scholar 

  7. J. DE Hart, G. Cacciola, P. J. G. Schreurs and G. W. M. Peter, J. Biomechanics 21 (1998) 629.

    Article  Google Scholar 

  8. M.-F. Guidoin, Y. Marois, J. Bejui, N. Poddevin, M. W. King and R. Guidoin, Biomaterials 21 (2000) 2461.

    Article  CAS  Google Scholar 

  9. J. C. Middleton and Arthur J. Tipton, Biomaterials 21 (2000) 2335.

    Article  CAS  Google Scholar 

  10. P. M. Kaufmann, S. Heimrath, B. S. Kim and D. J. Cell, Transplantation 6 (1997) 463.

    CAS  Google Scholar 

  11. Y. I. Jeong, J. G. Song, S. S. Kang, H. H. Ryu, Y. H. Lee, C. Choi, B. A. Shin, K. K. Kim, K. Y. Ahn and S. Jung, Int. J. Pharmaceutics 259 (2003) 79.

    Article  CAS  Google Scholar 

  12. Y. Tabata, Drug discovery today 6 (2001) 483.

    Article  CAS  Google Scholar 

  13. G. Beumer, C. van Blitterswijk and M. Ponec, J. Biomed. Matter Res. 28 (1994) 545.

    Article  CAS  Google Scholar 

  14. D. W. Hutmacher, Biomaterials 21 (2000) 2529.

    Article  CAS  Google Scholar 

  15. S. Ralf and S. P. Hoerstrup, The Annals of Thoracic Surgery 70 (2000) 140.

    Article  Google Scholar 

  16. D. W. Hutmacher, Biomaterials 21 (2000) 2529.

    Article  CAS  Google Scholar 

  17. M. Okada, Prog. Polym. Sci. 27 (2002) 87.

    Article  CAS  Google Scholar 

  18. A. Gonzalez, M. Iriarte, P. J. Iriondo and J. J. Iruin, Polymer 44 (2003) 7701.

    Article  CAS  Google Scholar 

  19. J. E. Oh, Y. S. Nam, K. H. Lee and T. G. Park, J. Controlled Release 57 (1999) 269.

    Article  CAS  Google Scholar 

  20. F.-L. MI, Y.-M. Lin, Y.-B. WU, S.-S. Shyu and Y.-H. Tsai, Biomaterials 23 (2002) 3257.

    Article  CAS  Google Scholar 

  21. M. S. Widmer, P. K. Gupta, L. LU, R. K. Meszlenyi, G. R. D. Evans, K. Brandt, T. Savel, A. Gurlek, C. W. Patrick, A. G. Mikos, Biomaterials 19 (1998) 1945.

    Article  CAS  Google Scholar 

  22. V. R. Sinha and R. Kumria, Int. Pharmaceutics 224 (2001) 19.

    Article  CAS  Google Scholar 

  23. J. Aburto, I. Alric, S. Thiebaud, E. Borredon, D. Bikiaris, J. Prinos and C. Panayiotou, J. Appl. Polym. Sci. 74 (1999) 1440.

    Article  CAS  Google Scholar 

  24. Q. Cai, Y. Wan, J Bei and S. Wang, Biomaterials 24 (2003) 3555.

    Article  CAS  Google Scholar 

  25. U. Edlund and A.-C. Albertsson, Advanced Drug Delivery Reviews 55 (2003) 585.

    Article  CAS  Google Scholar 

  26. R. D. Sonwalkar, C. C. Chen and L.-K. JU, Bioresource Technology 87 (2003) 69.

    Article  CAS  Google Scholar 

  27. C. Wang, H. LI and X. Zhao, Biomaterials 25 (2004) 5797.

    Article  CAS  Google Scholar 

  28. J. K. Kim, D.-J. Park, M.-S. Lee and K. J. Ihn, Polymer 42 (2001) 7429.

    Article  CAS  Google Scholar 

  29. S. LI and M. Vert, Macromolecules 36 (2003) 8008.

    Article  CAS  Google Scholar 

  30. J. S. Mao, H. F. Liu, Y. J. Yin and K. D. Yao, Biomaterials 24 (2003) 1621.

    Article  CAS  Google Scholar 

  31. Y. Luo, K. R. Kirker and G. D. Prestwich, J. Controlled Release 69 (2000) 169.

    Article  CAS  Google Scholar 

  32. M. G. Cascone, B. Sim and Sandra Downes, Biomaterials 16 (1995) 569.

    Article  CAS  Google Scholar 

  33. T. Groth and G. Altankov In: Cell-surface interactions and the tissue compatibility of biomedical materials, (DC: IOS Press, Washington, 1998) p. 12.

    Google Scholar 

  34. B. P. Toole, In: Cell Biology of Extracellular Matrix. (Hay E. ed., New York) (1981) p. 259.

  35. B. P. Toole In: Cell Biology of Extracellular Matrix, (Hay E. ed., New York) (1991) p. 305.

  36. Y. Wan, W. Chen, J. Yang, J. Bei and S. Wang, Biomaterials 24 (2003) 2195.

    Article  CAS  Google Scholar 

  37. M. E. Gomes, A. S. Ribeiro, P. B. Malafaya, R. L. Reis and A. M. Cunha, Biomaterials 22 (2001) 883.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering National Tsing Hua, University 101, Section 2 Kuang Fu Road, Hsin Chu, Taiwan, 300, ROC

    Chih-Ta Lee & Yu-Der Lee

Authors
  1. Chih-Ta Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu-Der Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu-Der Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, CT., Lee, YD. Preparation of porous biodegradable poly(lactide-co-glycolide)/ hyaluronic acid blend scaffolds: Characterization, in vitro cells culture and degradation behaviors. J Mater Sci: Mater Med 17, 1411–1420 (2006). https://doi.org/10.1007/s10856-006-0617-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-006-0617-5

Keywords

Search

Navigation