Abstract
Calcium phosphates, particularly hydroxyapatite Ca10(PO4)6(OH)2 (HA), are widely used for bone regeneration due to their biocompatibility and good resorption properties. However, their performance upon implantation is improved when they are associated with bioactive molecules such as growth factors. Using mesoporous HA leads to improved protein adsorption and release kinetics because the diameter of the mesopores (2–50 nm) is in the same range as their size. We prepared this type of material by the nanocasting method using three different templates: a silica foam and two carbon templates derived from it using propylene or sucrose as carbon source. We investigated the influence of the template, the calcination temperature and of the conditions during template removal. We obtained HA materials with a surface area of up to 90 m² g−1 and with an intergranular mesopore volume of up to 0.4 cm³ g−1. In this paper, we show for the first time that the synthesis of mesoporous HA from a mesoporous silica foam template allows eliminating the template at lower temperatures (in an alkaline medium), thus preventing the sintering of the HA. These materials have interesting properties for drug delivery applications. The protein adsorption and release capacities of these HAs were tested with two model proteins, bovine serum albumin (BSA), and Cytochrome C. These materials are an important milestone for future bone regeneration systems based on HA associated with human growth factor proteins.
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Murugan R, Ramakrishna (2005) s. Compos Sci Technol 65(15–16):2385
Oguchi H, Ishikawa K, Mizoue K, Seto K, Eguchi G (1995) Biomaterials 16(1):33
Laquerriere P, Grandjean-Laquerriere A et al (2205) ITBM RBM (now: IRBM) 26:200
Yang P, Quan Z et al (2008) Biomaterials 29:4341
Vuol J, Bohling T, Goransson H, Poulakkaien P (2002) J Biomed Mater Res 59:152
Zhu JH, Shi ML, Wang R, Chen X, Springer TA (2011) Nature 474:343
Mandal K, Kent SB (2011) Angew Chem Int Ed Engl 50(53):8029
Xia Z, Liao L, Zhao S (2009) Mater Res Bull 44:1626
Zhao Q, Wang T, Wang J, Zheng L, Jiang T, Cheng G, Wang S (2012) J Non-Cryst Solids 358:229
Poh CK, Ng S, Lim TY, Tan HC, Loo J, Wang W (2012) J Biomed Mater Res A 100A:3143
Yao J, Tjandra W, Chen YZ, Tam KC, Ma J, Soh B (2003) J Mater Chem 13:3053
Wang H, Zhai L, Li Y, Shi T (2008) Mater Res Bull 43:1607
Schmidt SM, McDonald J, Pinada ET, Verwilst AM, Chen Y, Josephs R, Ostafin AE (2006) Micro Meso 94:330
Yang P, Deng T, Zhao D, Feng P, Pine D, Chmelka BF, Whitesides GM, Stucky GD (1998) Science 282:2244
Ng S, Guo J, Ma J, Loo SCJ (2010) Acta Biomat 6:3772
Ye X, Guohua X, Cai S, Dou Y, Zhu Y, Hu H (2012) J Mater Sci 47:3763
Stein A (2001) Micro. Meso. 44–45:227
Melde BJ, Stein A (2002) Chem Mater 14:3326
Fujishima M, Okawa Y, Uchida K (2008) J Am Chem Soc 91:3749
Fan J, Lei J, Yu C, Tu B, Zhao D (2007) Mater Chem Phys 103:489
Moeller J, Dibandjo P et al (2010) Hard template synthesis of mesoporous hydroxyapatite materials for controlled protein release. In: Narajan R, Mc Kittric J (ed) Advances in bioceramics and biotechnologies, Book series: Ceramic Transactions 218, pp 37–42
Tiemann M (2007) Mater Chem 20(3):961
Schmidt-Winkel P Jr, Lukens WW, Yand P, Margolese DI, Lettow JS, Ying JY, Stucky GD (2000) Chem Mater 12:686
Liu D, Troczynski T, Tseng W (2001) Biomaterials 22:1721
Lukens WW Jr, Schmidt-Winkel P et al (1999) Langmuir 15:5403
Gregg SJ, Sing KSW (1982) Adsorption, surface area and porosity, 2nd edn. Academic Press, London
Rouquerol F, Rouquerol J, Sing K (1999) Adsorption by powders and porous solids. Academic Press, London, pp 439–447
Cao L, Dong H, Huang L, Matyjaszewski K, Kruk M (2009) Adsorption 15:156
Kruk M, Jaroniec M (2000) Chem Mater 12:1961
Parmentier J, Saadhallah S, Reda M, Gibot P, Roux M, Vidal L, Vix-Guterl C, Patarin J (2004) J Phy Chem Solids 65(2–3):139
Lu AH, Smatt J, Backlund S, Linden M (2004) Micro Meso 72:65
Narsimhan G (1961) Chem Eng Sci 16(1–2):7
Satterfield CN, Feakes F (1959) AIChE J 5(1):115
Alba-Simionesco C, Coasne B, Dosseh G, Dudziak G, Gubbins KE, Radhakrishnan R, Sliwinska-Bartkowiak M (2006) J Phys Condens Mater 18:R15
Huber M, Stark WJ et al (2005) Chem Comm 7:648
Shors EC, White EW, Kopchok G (1989) Mater Res Soc 110:211
Oghushi H, Okumura M, Yoshikawa T, Inboue K, Senpuku N, Tamai S, Shors EC (1992) J Biomed Mater Res 26(7):885
Patterson AL (1939) Phys Rev 56(10):978
Soler-Illia GJAA, Sanchez C, Lebeau B, Patarin J (2002) Chem Rev 102:4093
Wang H, Zhai L, Li Y, Shi T (2008) Mater Res Bull 43:1607
Ng S, Guo J, Ma J, Loo SCJ (2010) Acta Biomat 6:3772
Matsumoto T, Okazaki M, Inoue M, Yamaguchi S, Kusunose T, Toyonaga T (2004) Biomaterials 25:3807
Kandori K, Tsuyama S, Tanaka H, Ishikawa T (2007) Colloids Surf B 58(2):98
Hughes Wassell DT, Hall RC et al (1995) Biomaterials 18:1001
Ouizat S, Barroug A, Legrouri A, Rey C (1999) Mater Res Bull 34(14/15):2279
Yiu HHP, Botting CH, Botting NP, Wright PA (2001) Phys Chem Chem Phys 3:2983
Acknowledgements
The authors gratefully acknowledge the financial support by the ANR program NANOBONEFILLER (ANR-06-NANO-22). We also thank Simon Gree and Fabien Schnell for the small angle X-ray diffraction and Loic Vidal for transmission electron microscopy.
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Moeller-Siegert, J., Parmentier, J., Anselme, K. et al. Mesoporous hydroxyapatite by hard templating of silica and carbon foams for protein release. J Mater Sci 48, 3722–3730 (2013). https://doi.org/10.1007/s10853-013-7170-7
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DOI: https://doi.org/10.1007/s10853-013-7170-7