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Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To associate glucose-6-phosphate dehydrogenase (G6PDH) activity in goat oocytes with intracellular glutathione (GSH) content, meiotic competence, developmental potential, and relative abundance of Bax and Bcl-2 genes transcripts.

Methods

Goat oocytes were exposed to brilliant cresyl blue (BCB) staining test and categorized into BCB+ (blue-cytoplasm), and BCB− (colorless-cytoplasm) groups. A group of oocytes were not exposed to BCB test and was considered as a control group. After maturation in vitro, a group of oocytes were used for determination of nuclear status and intracellular GSH content while another group was subjected to parthenogenetic activation followed by in vitro embryo culture.

Results

We found that BCB+ oocytes not only yielded higher rate of maturation, but also showed an increased level of intracellular GSH content than BCB− and control oocytes. Furthermore, BCB+ oocytes produced more blastocysts than BCB− and control oocytes. Our data revealed that the expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) genes were interacted with G6PDH-activity in mature oocyte, their surrounding cumulus cells, and blastocyst-stage embryos.

Conclusions

The results of this study demonstrate that selection of goat oocytes based on G6PDH-activity through the BCB test improves their developmental competence, increases intracellular GSH content, and affects the expression of the apoptosis-related genes.

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Acknowledgments

This work was supported by Stem Cell Technology Research Center (Tehran, Iran). The authors thank the members of their teams for their technical expertise and support.

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Authors and Affiliations

  1. Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran

    Amir Hossein Abazari-Kia, Maryam Dehghani-Mohammadabadi & Fatemeh Jamshidi-Adegani

  2. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran

    Abdollah Mohammadi-Sangcheshmeh & Arash Veshkini

  3. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mahdi Zhandi

  4. Department of Animal Science, Faculty of Agriculture, Erciyes University, Kayseri, Turkey

    Mehmet Ulas Cinar

  5. Department of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Mohammad Salehi

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  1. Amir Hossein Abazari-Kia
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Correspondence to Mohammad Salehi.

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Capsule G6PDH-activity in goat oocytes was found to be associated with glutathione content, developmental competence, and expression of the apoptosis-related genes.

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Abazari-Kia, A.H., Mohammadi-Sangcheshmeh, A., Dehghani-Mohammadabadi, M. et al. Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte. J Assist Reprod Genet 31, 313–321 (2014). https://doi.org/10.1007/s10815-013-0159-y

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