Abstract
Objective
To compare the clinical outcomes between fresh and vitrified-thawed day 5 blastocyst transfers.
Design
Retrospective case control study.
Setting
Tertiary referral center.
Patient(s)
Patients 38 years of age or less who underwent IVF/ICSI cycles with fresh or frozen-thawed blastocysts transferred from June 1, 2009 to November 30, 2011
Intervention(s)
Vitrification and thawing of day 5 blastocysts using the Cryotop method. (Kitazato BioPharma Co., Ltd., Fuji city, Shizuoka, Japan)
Main outcome measure(s)
Clinical pregnancy rate, implantation rate, ongoing pregnancy rate, and multiple pregnancy rates.
Results
Of the 118 cycles in the fresh transfer group, 234 blastocysts were transferred. The clinical pregnancy rate was 66.1 % and implantation rate was 50.9 %. The ongoing pregnancy rate was 56.8 % and the rates for singleton and twin pregnancies were 53.7 % and 44.8 %. Of the 59 cycles in the vitrified-thawed group, 111 blastocysts were transferred. The clinical pregnancy rate was 59.3 % and implantation rate was 43.2 %. The ongoing pregnancy rate was 47.5 % and the rates for singleton and twin pregnancies were 60.7 % and 39.3 %. The clinical pregnancy rate, implantation rate and ongoing pregnancy rate did not differ significantly between the two groups.
Conclusions
The implantation rates were not significantly different between the fresh and the vitrified-thawed groups. Thus, single embryo transfer may be considered in fresh cycles to decrease multiple pregnancy rates. The surplus embryos should be vitrified for the frozen embryo transfer to improve the cumulative pregnancy rate.
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References
Gardner DK, Balaban B. Choosing between day 3 and day 5 embryo transfers. Clin Obstet Gynecol. 2006;49:85–92.
Kuleshova LL, Lopata A. Vitrification can be more favorable than slow cooling. Fertil Steril. 2002;78:449–54.
Chang EM, Han JE, Kim YS, et al. Use of the natural cycle and vitrification thawed blastocyst transfer results in better in-vitro fertilization outcomes. J Assist Reprod Genet. 2011;28:369–74.
Aflatoonian A, Oskouian H, Ahmadi S, Oskouian L. Can fresh embryo transfers be replaced by cryopreserved-thawed embryo transfers in assisted reproductive cycles? A randomized controlled trial. J Assist Reprod Genet. 2010;27:357–63.
Shapiro BS, Daneshmand ST, et al. Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen–thawed embryo transfer in normal responders. Fertil Steril. 2011;96:344–8.
Hong SW, Sepilian V, Chung HM, Kim TJ. Cryopreserved human blastocysts after vitrification result in excellent implantation and clinical pregnancy rates. Fertil Steril. 2009;92:2062–4.
Fatemi HM, Kyrou D, Bourgain C, et al. Cryopreserved-thawed human embryo transfer: spontaneous natural cycle is superior to human chorionic gonadotropin–induced natural cycle. Fertil Steril. 2010;94:2054–8.
Zegers-Hochschild F, et al. International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil Steril. 2009;92:1520–4.
Kuc P, Kuczyñska A, et al. Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocyte retrieval and IVF outcomes. Folia Histochem Cytobiol. 2010;48:84–8.
Rall WF, Fahy GM. Ice-free cryopreservation of mouse embryos at −196 °C by vitrification. Nature. 1985;313:573–5.
Larman MG, Katz-Jaffe MG, et al. Analysis of global gene expression following mouse blastocyst cryopreservation. Hum Reprod. 2011;10:2672–80.
Zhu D, Zhang J, Cao S, et al. Vitrified-warmed blastocyst transfer cycles yield higher pregnancy and implantation rates compared with fresh blastocyst transfer cycles—time for a new embryo transfer strategy? Fertil Steril. 2011;95:1691–5.
Haouzi D, Assou S, Mahmoud K, et al. Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the same patients. Hum Reprod. 2009;24:1436–45.
Haouzi D, Assou S, Dechanet C, et al. Controlled ovarian hyperstimulation for in vitro fertilization alters endometrial receptivity in humans: protocol effects. Biol Reprod. 2010;82:679–86.
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Pei-Yun Ku and Robert Kuo-Kuang Lee contributed equally to this work.
Capsule
Vitrified blastocysts have similar implantation potential in comparison to fresh blastocysts.
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Ku, PY., Lee, R.KK., Lin, SY. et al. Comparison of the clinical outcomes between fresh blastocyst and vitrified-thawed blastocyst transfer. J Assist Reprod Genet 29, 1353–1356 (2012). https://doi.org/10.1007/s10815-012-9874-z
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DOI: https://doi.org/10.1007/s10815-012-9874-z