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The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media

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Abstract

Purpose

This study was to investigate the effect of sodium selenite (SS) on in vitro maturation of mouse preantral follicles.

Methods

The isolated preantral follicles were cultured in TCM 199 medium supplemented with different concentrations (0, 5, 10, 15 ng/ml) of SS and 3 mg/ml bovine serum albumin (BSA) or 5% Fetal Bovine Serum (FBS). The ovulation was induced by addition of 1.5 IU/ml human chorionic gonadotropin. The size and development of follicles and oocytes were assessed by calibrated eyepiece.

Results

The survival rates of follicles in FBS supplemented groups containing 5 and 10 ng/ml SS (88.23%, 90.83%) were higher than other groups (P < 0.05 and P < 0.001 respectively). The mean diameter of follicles (199.84 ± 15.58 μm) and the percentage of MII oocyte (33.08%) were higher in FBS supplemented group containing 10 ng/ml SS (P < 0.001).

Conclusion

The sodium selenite and FBS improve the in vitro growth and maturation of mouse preantral follicles.

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Acknowledgements

We thank Mr. Pour Beyranvand and Ms, Ebrahimi for providing excellent technical assistance. This work was supported by a grant from Tarbiat Modares University and Iran National Science Foundation.

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Authors and Affiliations

  1. Department of Anatomy, Tarbiat Modares University, P. O. BOX: 14115-111, Tehran, Iran

    A. Abedelahi & M. Salehnia

  2. Department of Biochemistry, Tarbiat Modares University, Tehran, Iran

    A. A. Allameh

Authors
  1. A. Abedelahi
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  2. M. Salehnia
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  3. A. A. Allameh
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Corresponding author

Correspondence to M. Salehnia.

Additional information

Capsule Sodium selenite at concentrations of 10 ng/ ml in FBS supplemented media increases the in vitro maturation of mouse preantral ovarian follicle.

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Abedelahi, A., Salehnia, M. & Allameh, A.A. The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media. J Assist Reprod Genet 25, 483–488 (2008). https://doi.org/10.1007/s10815-008-9252-z

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  • DOI: https://doi.org/10.1007/s10815-008-9252-z

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