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Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes

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Abstract

Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.

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Funding

We are grateful to Centro de Inseminación Artificial La Elisa S.A. (CIALE) for providing bovine frozen semen, and the Staff of SENASA from Frigorífico Gorina S.A. for providing the bovine ovaries. This work was supported by Grant PICT 2015-2154 from Agencia Nacional de Promoción Científica y Tecnológica de la República Argentina (MINCyT). Thanks are also due to A. Di Maggio for manuscript correction and edition.

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Authors and Affiliations

  1. IGEVET – Instituto de Genética Veterinaria “Ing. Fernando N. Dulout” (UNLP-CONICET LA PLATA) Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118s/n, CP 1900, La Plata, Buenos Aires, Argentina

    Raúl Martín Lizarraga, Juan Mateo Anchordoquy, Esteban Martín Galarza, Nicolás Agustín Farnetano, Ana Carranza-Martin, Cecilia Cristina Furnus, Guillermo Alberto Mattioli & Juan Patricio Anchordoquy

  2. Cátedra de Fisiología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118s/n, CP 1900, La Plata, Buenos Aires, Argentina

    Juan Mateo Anchordoquy, Esteban Martín Galarza, Guillermo Alberto Mattioli & Juan Patricio Anchordoquy

  3. Cátedra de Citología, Histología y Embriología “A” Facultad de Ciencias Médicas Médicas, Universidad Nacional de La Plata, calle 60 y 120s/n, CP 1900, La Plata, Buenos Aires, Argentina

    Cecilia Cristina Furnus

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  1. Raúl Martín Lizarraga
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  2. Juan Mateo Anchordoquy
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  7. Guillermo Alberto Mattioli
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Contributions

R.M.L. and J.M.A. designed the study, E.M.G., N.A.F., and A.C-M. assisted with data collection. C.C.F. and G.A.M analyzed the data and J.P.A. coordinated the experiments and revised the manuscript. All co-authors participated in lab work.

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Correspondence to Juan Patricio Anchordoquy.

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Lizarraga, R.M., Anchordoquy, J.M., Galarza, E.M. et al. Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes. Biol Trace Elem Res 197, 149–158 (2020). https://doi.org/10.1007/s12011-019-01966-2

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