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Fluorescence spectral analysis of thioflavin T–γ-cyclodextrin interaction

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Journal of Applied Spectroscopy Aims and scope

Fluorescence spectra and lifetimes of thioflavin T (ThT) in aqueous solutions of γ-cyclodextrin (γ-CD) were studied. Enhancements of the probe fluorescence intensity and lifetime were shown to result from the formation of two types (1:1 and 1:2) of ThT–γ-CD inclusion complexes. The formation constants of these complexes were determined based on spectral-kinetic measurements. Proof was provided that the probe did not form dimers at low concentrations (10−6–10−5 M).

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Authors and Affiliations

  1. Yanka Kupala Grodno State University, 22 Ozheshko Str., Grodno, 230023, Belarus

    A. A. Maskevich, S. A. Kurhuzenkau & A. Yu. Lickevich

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  1. A. A. Maskevich
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  2. S. A. Kurhuzenkau
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  3. A. Yu. Lickevich
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Correspondence to A. A. Maskevich.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 80, No. 1, pp. 39–45, January–February, 2013.

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Maskevich, A.A., Kurhuzenkau, S.A. & Lickevich, A.Y. Fluorescence spectral analysis of thioflavin T–γ-cyclodextrin interaction. J Appl Spectrosc 80, 36–42 (2013). https://doi.org/10.1007/s10812-013-9717-4

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  • DOI: https://doi.org/10.1007/s10812-013-9717-4

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