Abstract
The demand for vegetable proteins increases globally and seaweeds are considered novel and promising protein sources. However, the tough polysaccharide-rich cell walls and the abundance of polyphenols reduce the extractability and digestibility of seaweed proteins. Therefore, food grade, scalable, and environmentally friendly protein extraction techniques are required. To date, little work has been carried out on developing such methods taking into consideration the structural differences between seaweed species. In this work, three different protein extraction methods were applied to three Swedish seaweeds (Porphyra umbilicalis, Ulva lactuca, and Saccharina latissima). These methods included (I) a traditional method using sonication in water and subsequent ammonium sulfate-induced protein precipitation, (II) the pH-shift protein extraction method using alkaline protein solubilization followed by isoelectric precipitation, and (III) the accelerated solvent extraction (ASE®) method where proteins are extracted after pre-removal of lipids and phlorotannins. The highest protein yields were achieved using the pH-shift method applied to P. umbilicalis (22.6 ± 7.3%) and S. latissima (25.1 ± 0.9%). The traditional method resulted in the greatest protein yield when applied to U. lactuca (19.6 ± 0.8%). However, the protein concentration in the produced extracts was highest for all three species using the pH-shift method (71.0 ± 3.7%, 51.2 ± 2.1%, and 40.7 ± 0.5% for P. umbilicalis, U. lactuca, and S. latissima, respectively). In addition, the pH-shift method was found to concentrate the fatty acids in U. lactuca and S. latissima by 2.2 and 1.6 times, respectively. The pH-shift method can therefore be considered a promising strategy for producing seaweed protein ingredients for use in food and feed.
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Introduction
By the year 2050, the global human population is estimated to reach 9.7 billion (United Nations 2017), and the food requirements are estimated to be 60% higher (Alexandratos and Bruinsma 2012) than those today. In a review by Aiking (2014), it is argued that a decrease in animal-based proteins is a prominent option to reach a more sustainable food supply chain in the future. Therefore, there is a requirement for new sources of vegetable proteins. Certain seaweeds are rich in proteins, e.g., some red seaweed species are reported to contain up to 47% protein (dry weight, dw, basis) (Fujiwara-Arasaki et al. 1984; Černá 2011). The productivity of seaweed beds can also be several times higher than that in terrestrial systems (Leigh et al. 1987), making seaweeds a promising source for vegetable proteins.
In addition to the high protein content, seaweeds contain other interesting compounds from a food point of view. Even if the fat content of seaweeds is low, it consists of high levels of long-chain n3 polyunsaturated fatty acids (LC n3 PUFA) and especially red seaweed species can contain high levels of eicosapentaenoic acid (EPA) (Fleurence et al. 1994; Matanjun et al. 2009; Cian et al. 2014; Maehre et al. 2014; Rodrigues et al. 2015). For example, Fleurence et al. (1994) reported that EPA constitutes 48% of the total fatty acid content in Porphyra umbilicalis. Also, docosahexaenoic acid (DHA) has been reported in low amounts in some red (Fleurence et al. 1994), green (Ortiz et al. 2006) (i.e., Ulva lactuca), and brown (Ortiz et al. 2006; Matanjun et al. 2009; Veide Vilg et al. 2015) seaweed species. However, for the brown seaweed Saccharina latissima, the DHA levels vary with both location and season of harvest but can make up 8.5% of the total fatty acid content (Veide Vilg et al. 2015). Seaweeds are also high in minerals, with reported ash levels for most species varying between ~ 20 and 40% dw (Wong and Cheung 2000; Rupérez 2002; Aguilera-Morales et al. 2005; Ortiz et al. 2006; Matanjun et al. 2009; Peña-Rodríguez et al. 2011; Yaich et al. 2011; Maehre et al. 2014; Rodrigues et al. 2015; Veide Vilg et al. 2015); however, both lower (Ortiz et al. 2006; Cian et al. 2014; Veide Vilg et al. 2015) and higher (Matanjun et al. 2009; Peña-Rodríguez et al. 2011; Maehre et al. 2014) values have been reported. The high abundance of minerals could be interesting from a food perspective for use as salt replacers and/or as a source of important minerals (Rupérez 2002).
Seaweeds have a very tough polysaccharide-rich cell wall and the cell wall mucilage reduces the extractability of proteins (Fleurence et al. 1995). The extractability of proteins is affected both by the high viscosity that the polysaccharides exert in a water solution and by the ionic interactions between the cell wall and the proteins (Joubert and Fleurence 2008). Also, the abundance of phenols, such as phlorotannins in brown seaweeds (Hurd et al. 2014), can decrease the extractability further (Jordan and Vilter 1991) as well as reducing the digestibility of the proteins (Wong and Cheung 2001; Ozdal et al. 2013) by binding the proteins either covalently or by non-covalent forces (Jordan and Vilter 1991; Stern et al. 1996; Ozdal et al. 2013).
To separate proteins from polysaccharides and phenolic compounds and, at the same time, improve the protein content and sensory properties, food grade, scalable, and environmentally friendly techniques for extracting seaweed proteins are desirable. Most reported techniques for extraction of proteins from seaweeds utilize water only (i.e., Galland-Irmouli et al. (1999) and Garcia-Vaquero et al. (2017)) or a water extraction followed by a second alkaline extraction in the presence of mercaptoethanol (i.e., Wong and Cheung (2001), Kandasamy et al. (2012), and Suresh Kumar et al. (2014)) followed finally by precipitation with ammonium sulfate. The pH-shift process (Fig. 1), with alkaline protein solubilization followed by isoelectric precipitation, commonly used for extraction of protein from fish muscle (Undeland et al. 2002; Marmon and Undeland 2010), was recently applied to S. latissima by Veide Vilg and Undeland (2017) with the addition of an osmotic shock step. In this study (Veide Vilg and Undeland 2017), the total protein yield was 16%. By using accelerated solvent extraction (ASE®), also known as pressurized liquid extraction (PLE), which combines temperature and pressure to generate extracts using food grade solvents, proteins are extracted after pre-removal of lipids and phlorotannins. A review by Herrero et al. (2015) discusses how ASE® was previously used for extraction of different bioactive compounds from seaweeds and microalgae.
Since the composition and structure of different seaweeds vary largely (Hurd et al. 2014), different species are expected to respond differently to different protein extraction procedures. A careful mapping of the best process-species combinations would be of great help when building future seaweed bio-refineries. To our knowledge, no study carried out to date compares different protein extraction methods on red, green, and brown seaweeds and therefore the aims of this work were to (I) generate protein extracts from P. umbilicalis, U. lactuca, and S. latissima using three different extraction methods including a traditional method using ammonium sulfate salting out, the pH-shift method, and the ASE® method; (II) determine the yield of protein obtained; and (III) determine the quality of the obtained protein extracts in terms of their crude composition and their amino acid and fatty acid composition.
Materials and method
Chemicals
Diethyl ether, Folin-Ciocalteu phenol, petroleum ether, sodium carbonate, sodium chloride, sodium dodecyl sulfate, and sodium tartrate were from Sigma-Aldrich (Sweden). Sodium hydroxide and toluene were from Scharlau (Spain). Acetic acid was from Fischer Scientific (Sweden), acetyl chloride and copper sulfate were from Fluka (Switzerland), hydrochloric acid was from Acros (Sweden), and methanol was from Honey well Riedel-de Haen (Sweden). Acetone, ammonium sulfate, hexane, and methanol were from Sigma-Aldrich (Ireland).
Seaweed raw material
Porphyra umbilicalis tufts were collected at Inre Vattenholmen (58° 52.622 N; 11° 6.876 E) on 2 April 2016 and rinsed several times in natural seawater to remove grazers and loose epiphytes. Tufts were then placed into cultivation tanks (see below).
Cultivated gametophytes from U. lactuca were fragmented and subjected to temperature shock to induce the production of reproductive tissue. After 2 days, released gametes germinated and attached to sterile petri dishes. After 2 months in petri dishes, juvenile U. lactuca were placed into cultivation tanks (see below).
Adult S. latissima sori were dried and subjected to cold temperature shock overnight. In the morning, sori were submerged in seawater and spore release occurred shortly after. After 2 months in petri dishes, juvenile S. latissima were placed into cultivation tanks.
All seaweeds were cultivated at the Sven Loven Center for Marine Infrastructure at Tjärnö, Sweden (58° 52′ 33.2724″ N, 11° 8′ 47.202″ E), in 80-L cultivation tanks at 12 °C and with a neutral light-cycle (12-h daylights, 12-h darkness) at light intensity of 50 μmol photons m−2 s−1. Seaweeds received continuous filtered seawater. The lowest filter size used was 1 μm and all seawater was treated with UV prior to entering the cultivation tanks. No additional medium was added.
Extraction of proteins
Proteins were extracted from freeze-dried and milled P. umbilicalis, U. lactuca, and S. latissima biomass using three different methods. The total protein yield for respective species/method combinations was calculated according to the following equation: 100 × ((protein content in extract × amount extract achieved)/(protein content in crude seaweed × amount seaweed used)). Thus, the total yield is the protein obtained in the different processes divided by the amount ingoing protein. The total protein content was analyzed with the Lowry method (Lowry et al. 1951) as further modified by Markwell et al. (1978) and as total amount amino acids. Compositional analysis was performed in triplicate on extracts from each process type.
Traditional protein extraction using sonication in water and ammonium sulfate precipitation of protein
Seaweed proteins were extracted using the method previously described by Galland-Irmouli et al. (1999). Briefly, 10 g of freeze-dried and milled seaweed was independently suspended in 1 L of ultrapure Milli-Q water. Following ultra-sonication for 1 h, the seaweed solution was left to stir overnight on a magnetic stirrer plate at 4 °C. The solution was then centrifuged at 10000×g for 1 h and the supernatant decanted. The pellet fraction was suspended in 200 mL of Milli-Q water and subjected to a second extraction procedure as described above. Both supernatants were pooled together for each seaweed species and subsequently brought to 80% (w/v) ammonium sulfate saturation, stirred for 1 h at 4 °C, and centrifuged at 20,000×g for 1 h to precipitate the protein fraction. The precipitates were subsequently dialyzed using 3.5-kDa MWCO dialysis tubing (Fischer Scientific, USA) against Milli-Q water at 4 °C overnight. The precipitates were subsequently freeze-dried and stored at − 80 °C until further use. The traditional protein extraction method was performed in duplicate for all species and one of the produced extracts was used for compositional analyses.
pH-shift protein extraction
Proteins were extracted from seaweeds using the pH-shift method according to Veide Vilg and Undeland (2017). However, the method first had to be adapted for U. lactuca and P. umbilicalis by determining the solubility maxima and precipitation maxima of the proteins. Those experiments were performed in duplicate.
Adaption of the pH-shift process to Porphyra umbilicalis and Ulva lactuca
According to Veide Vilg and Undeland (2017), biomass was homogenized in distillated water in a 1:4 (w/v) ratio based on the wet weight of each seaweed species, and stirred for 1 h at 8 °C. The protein content in the homogenate was analyzed using the method of Lowry et al. (1951) (see below). To determine the maximum solubility, the pH of the seaweed water mix was stepwise adjusted from its native pH (6.1 ± 0.1 and 4.8 ± 0.1 for P. umbilicalis and U. lactuca, respectively) either upwards (with 1 M NaOH) to a maximum value of pH 13, or downwards (with 1 M HCl) to a minimum value of pH 2. At each pH examined, sub-samples were withdrawn, centrifuged at 8000×g for 10 min and the protein content analyzed in supernatant 1 (Fig. 1) according to Lowry et al. (1951). The solubility at each pH step was calculated according to Eq. 1.
The precipitation maxima were then determined by adjusting the pH of the supernatant recovered at pH 12 (which gave the highest solubility) to pH values between pH 2 and 7 (for P. umbilicalis) and between pH 2 and 6 (for U. lactuca), followed by a second centrifugation at 8000×g for 10 min. Sub-samples were withdrawn from supernatant 1 at each pH both before centrifugation and after the second centrifugation step, i.e., from supernatant 2. The protein content of these samples was analyzed according to Lowry et al. (1951) (see below). The precipitation at each pH step was calculated according to Eq. 2.
To increase the protein yield further during precipitation, a freezing step was applied to supernatant 1 after the pH was decreased. The effect of this step was also examined for S. latissima. Further, to increase the overall yield, extraction with a larger volume of water (1:6 (w/v), based on the wet weight of each seaweed species) was investigated.
Production of protein extracts with the adapted pH-shift methods
By using the adapted pH values for solubilization and precipitation for P. umbilicalis and U. lactuca and the ones determined by Veide Vilg and Undeland (2017) for S. latissima, protein extracts were produced for the three seaweed species by homogenizing each dry-milled seaweed independently in distilled water in a 1:6 (w/v) ratio, based on the original wet weight of each seaweed species. Homogenization was done with an Ultra Turrax T18 basic (IKA, Germany) for 2 min at a speed of 4 (18,000 rpm) followed by milling with beads using a Retsch MM 400 at speed 1/30 s for 2 min. The homogenized sample was stirred for 1 h at 8 °C and thereafter the pH was adjusted to 12 (which gave the highest protein solubility) while the sample was kept on ice. Following centrifugation at 8000×g for 10 min, the supernatant containing the solubilized proteins was decanted and the pH was adjusted to pH 2 and the supernatant was frozen overnight at − 20 °C. After thawing and a second centrifugation at 8000×g for 10 min, the pellet was collected, freeze dried, and stored at − 80 °C until further analysis. The pH-shift protein extraction method was performed in duplicate for all species with the produced extracts being pooled for each species before compositional analysis.
Accelerated solvent extraction
ASE® extraction was carried out to extract lipids, phlorotannins, and carbohydrates prior to extraction of proteins as was previously described by Lopes et al., (2012). Seaweed lipids were removed using hexane. De-fatted seaweed material was left to dry overnight in a fume hood at room temperature (RT). This material was subsequently used as the substrate for carbohydrate/phlorotannin extraction using ASE®. ASE® was performed using the Dionex PLE System (ASE 200, Dionex, Germany) according to the previously published method (Lopes et al., 2012) with some modifications. The phlorotannin/carbohydrate fractions were generated according to the method of Lopes et al. (2012) with minor modifications as follows: 1 g of each freeze-dried and de-fatted powdered seaweed was mixed with 4 g of silica (Merck grade, 60 Å, Sigma-Aldrich, USA). Silica was used as an inert dispersant and the sample plus silica mixture was then packed into 22-mL extraction cells. The automated extraction method used 70% food grade acetone in water and a pressure and temperature of 1000 psi and 0 °C, respectively. The extraction time consisted of 1 cycle of 7 min. The recovered fractions were subsequently frozen at − 80 °C and evaporated to dryness at 30 °C under pressure before being freeze-dried using an industrial scale FD80 model freeze-drier (Cuddon Engineering, New Zealand) for 24 h to eliminate residual water. The seaweed/silica residue was used for protein extraction using ASE®. ASE® protein extraction was carried out by mixing 1 g of air-dried seaweed/silica residue with 4 g of silica which was packed into 22-mL extraction cells. The automated extraction method used 50% methanol in water and a pressure and temperature of 1500 psi and 37 °C, respectively. The extraction time consisted of 2 cycles of 5 min. The recovered fractions were dried using rotary evaporation to remove methanol, frozen at − 80 °C, and freeze-dried for 24 h to eliminate water as previously described. Protein fractions were then stored at − 80 °C until compositional analysis was performed. The ASE protein extraction technique was performed as singlets for each seaweed species.
Total proteins
The total protein content in the seaweeds and seaweed protein extracts was determined according to the method of Lowry et al. (1951) modified by Markwell et al. (1978). Twenty milligram of each dried extract or dried seaweed was vortexed independently in 1 mL 0.1 M NaOH and diluted further between 10 and 100 times with 0.1 M NaOH. For homogenates and supernatants, 0.1 mL was mixed with 0.9 mL NaOH and vortexed before eventual further dilution (up to 20 times). To a 1-mL sample, 3 mL of freshly made reagent (1 part 4% CuSO4·5H2O into 100 parts of a mixture of 2.0% Na2CO3, 0.40% NaOH, 0.16% Na-tartrate, and 1% SDS) was added before incubation for 30 min at RT. Thereafter, 0.3 mL freshly made phenol reagent (1 part Folin-Ciocalteu phenol reagent into 1 part Milli-Q water) was added and the samples were incubated for 45 min in darkness at RT. A Cary60 UV-VIS spectrophotometer (Agilent Technologies, USA) was used for absorbance measurements at 750 nm. A standard curve made from bovine serum albumin (BSA) was used for quantification.
Amino acids
Amino acid analysis was carried out using a modified version of the method of Özcan and Şenyuva (2006). To 100 mg of dry protein extract or dry seaweed, 10 mL of 6 M HCl was added and thereafter hydrolysis was carried out at 110 °C for 24 h. One milliliter of each of the hydrolyzed samples was dried by flushing with air and subsequently re-dissolved in 1 mL 0.2 M HAc. Five microliter was injected onto the LC-MS system consisting of an Agilent 1260 HPLC with a binary pump, an auto sampler, and a column oven with a Phenomenex column (C18 (2) 250 μm × 4.6 μm × 3 μm), coupled to an Agilent 6120 quadrupole in the SIM positive mode (Agilent Technologies, Germany). As mobile phase A, 3% MeOH, 0.2% formic acid, and 0.01% HAc were used and as mobile phase B, 50/50 MeOH/H20 with 0.2% formic acid and 0.01% HAc were used. The initial gradient, held for the first 8 min, was 94% of A and 6% of B. The gradient was then gradually changed until it reached 80% of A and 20% of B after 20 min. This gradient was held until 27 min, changed gradually until it reached 94% A and 6% B after 28 min, and thereafter held the rest of the run (total 40 min). As standard curve, a mix consisted of 24 amino acids diluted in 0.2 m HAc in the range 1–20 ppm was used. Due to the use of acidic hydrolysis, tryptophan could not be analyzed. Results are expressed as percent total amino acids in the seaweeds and extracts on a dry weight basis, and as percent of each amino acid out of the total amount of amino acids.
Fatty acids
Fatty acids were directly trans-esterified according to Cavonius et al. (2014). An internal standard (C17:0) in toluene was added to 25 mg of freeze-dried extract or dried seaweed. One milliliter of toluene and 1 mL of 10% HCl in methanol were added before the sample was incubated at 70 °C for 2 h. The reaction was stopped by adding 0.2 mL Milli-Q water. Five milliliter of ether solvent (petroleum ether 80% v/v and diethyl ether 20% v/v) was added and the sample was vortexed. After centrifugation (2500×g for 6 min), the organic phase was evaporated under nitrogen and re-dissolved in isooctane and injected onto an Agilent 7890 A GC system equipped with a VF-WAX column (30 cm × 0.250 mm × 0.25 μm) (Netherlands) and interfaced with an Agilent 5975 C triple-axis MS detector (USA) in electron impact mode. The injection volume was 1 μL with a 15:1 split at an inlet temperature of 275 °C. The carrier gas was helium, with a fixed flow of 1 mL min−1. The temperature started at 100 °C and increased by 4 °C min−1 to 205 °C and was thereafter increased further with 1 °C min−1 to 230 °C, and kept for the final 5 min. Fatty acids were quantified against the internal standard. Identification of fatty acids was done by using GLC Reference Standard 463 from Nu-Chek Prep, Inc., except for C16:1 n9 C16:2 n9, C16:4 n3, C18:4 n3, and C20:4 n3 which was determined using the MS-library. Results are expressed as percent total fatty acids in the seaweeds and extracts on a dry weight basis, and as percent of each fatty acid out of the total amount of fatty acids.
Ash
The ash content was determined by combusting 50 mg of either dry protein extract or dry seaweed at 550 °C for 3 h in a furnace. The samples were left in the oven to cool to 300 °C, further cooled in a desiccator and thereafter the weight was recorded. Results are expressed as percent ash on a dry weight basis.
Ionic strength
The ionic strength during the pH-shift process was determined by using a CDM210 conductivity meter (MeterLab, France). Sodium chloride (NaCl) was used as a standard and the results are expressed as percent NaCl equivalents in homogenates.
Statistical analyses
Data on protein yield for the extracts obtained with the traditional and pH shift extraction methods from P. umbilicalis, U. lactuca, and S. latissima, measured as total amino acids and with the Lowry method, were statistically analyzed using a three-way analysis of variance (ANOVA) with extraction method (2 levels), species (3 levels), and protein analysis (2 levels) as fixed, orthogonal factors (Table 1). Data on protein yield from the ASE® extraction method were not included in the statistical analysis because only one extract was obtained from each seaweed species. However, data is included in Fig. 3 for visual comparison.
Data on the total amino acid, total fatty acid, and ash content (% dw) in the crude seaweed material, as well as in the protein extracts obtained with the traditional, pH-shift, and ASE® extraction methods from P. umbilicalis, U. lactuca, and S. latissima were statistically analyzed using a two-way ANOVA with analyzed material (4 levels) and species (3 levels) as fixed, orthogonal factors. The residual degrees of freedom (df) in the ANOVAs were adjusted to account for missing replicates being replaced with the mean values (see Table 2 and Fig. 4) (Underwood 1997). Means were compared using the Student-Newman-Keuls (SNK) procedure (Underwood 1997).
Results
Adaptation of the pH-shift technique to Ulva lactuca and Porphyra umbilicalis
The solubility of proteins increased for U. lactuca with increased pH within the studied range (pH 2–13) (Fig. 2). However, to reach a pH over 12 required the use of a large amount of base (approximately 50 times more base was needed between pH 12 and 13 than between pH 6 and 7) and therefore pH 12 was selected as the most appropriate pH for solubilization of U. lactuca proteins. The solubility here was 62.1 ± 5.1%. The solubility of P. umbilicalis proteins also increased with increased pH and reached a maximum of 54.2 ± 4.9% at pH 12 (Fig. 2). Ionic strength in the U. lactuca homogenate upon solubilization at pH 12 was 74 mM NaCl equivalents. For P. umbilicalis, it was 83 mM NaCl equivalents.
a Protein solubility of Porphyra umbilicalis and Ulva lactuca in four volumes of water (based on the wet weight) at different pHs in the pH-shift method. b Protein precipitation for Porphyra umbilicalis and Ulva lactuca at different pHs when using the pH-shift method with initial solubilization at pH 12 followed by centrifugation at 8000×g (note that the y-axes in panels a and b differ)
The highest protein precipitation for both P. umbilicalis and U. lactuca (Fig. 2), after previous solubilization at pH 12 and centrifugation at 8000×g for 10 min, was achieved at pH 2; 33.3 ± 0.9% and 22.7 ± 8.01%, respectively. Applying a freezing step to supernatant 1 after adjustment to pH 2 increased the precipitation of P. umbilicalis and U. lactuca proteins further, by 8, respectively, 4 percentage points. For S. latissima, it increased the precipitation by 14 percentage points.
The calculated total protein yield over the whole pH-shift process for P. umbilicalis and U. lactuca was 13.7 ± 0.2% and 6.6 ± 2.5%, respectively, based on protein measurements in the two supernatants. However, the yield in the precipitation step of U. lactuca varied a lot, resulting in variances also in total protein yield. Extracting proteins from P. umbilicalis using a larger volume of water (1:6 w/v as opposed to 1:4 w/v) increased the total protein yield by 8 percentage points (from 13.7 to 21.7%) and adding the freezing-thawing step during precipitation enhanced the yield further by 4 percentage points (from 21.7 to 26.0%). For U. lactuca, the general variation in protein yield overshadowed the effect achieved using the larger extraction volume; however, adding of the freeze-thawing step gave a slightly higher yield (2 percentage points increase, from 6.4 to 8.1%). When using the previously recommended pH values for solubilization (pH 12) and precipitation (pH 2) for S. latissima (Veide Vilg and Undeland 2017) and combining this with a larger extraction volume (1:6 as opposed to 1:4) and a freezing-enhanced precipitation step, the total calculated protein yield achieved was 33% based on protein measurements in the supernatants.
Protein yield with the three protein extraction procedures
When data for the total protein yield for extracts from different seaweed species obtained using different extraction methods were statistically analyzed, we found a significant interaction between the factors species and extraction method (Table 1). The total protein yield was significantly (SNK, p < 0.05) higher for the extracts obtained with the pH-shift method for P. umbilicalis and S. latissima, compared to that of the extracts obtained with the traditional method (Fig. 3a, b). For the extracts from U. lactuca, a non-significant (SNK, p > 0.05) trend towards a higher protein yield in the extract obtained with the traditional method, compared to the pH-shift method, was found (Fig. 3a, b). The ASE® method was only used once to obtain extracts from the three seaweed species, and therefore the data could not be included in the statistical analysis. However, the total protein yield for these extracts was lower compared to the mean values for the extracts obtained with the traditional and the pH shift methods (Fig. 3a, b).
Total protein yield, analyzed with a the Lowry method and b as total amino acids, for extracts from Porphyra umbilicalis, Ulva lactuca, and Saccharina latissima generated with the traditional method, the pH-shift method, and the accelerated solvent extraction (ASE) method. Letters above bars indicate significant differences based on the Student-Newman-Keuls procedure. Error bars show SEM (n = 2). Only one extract was obtained using the ASE method (n = 1) and, therefore, no error bars are presented and the results were not included in the statistical analysis
We also detected a significant difference in the total protein yield estimated with the different analysis methods (Table 1). The Lowry method (Fig. 3a) consistently resulted in significantly (SNK, p < 0.05) higher total protein yield estimates compared to when the protein yield was estimated based on analysis of total amino acids (Fig. 3b). On average, the Lowry method resulted in 34.3% higher estimates of total protein yield compared to the analysis of total amino acids. These results indicate the importance of the chosen protein measurement method selected for protein analysis as highlighted recently by Maehre et al. (2018).
Composition of seaweed biomass and protein extracts
When data from the different compositional analyses in crude seaweed material and in extracts obtained with different extraction methods were statistically analyzed, we found highly significant interactions between the main factors—species and extracted material (Table 2). There were statistically significant differences (SNK, p < 0.05) in the total amino acid and fatty acid content, as well as ash content, between all the extracted materials, but the size of the effect differed between different seaweed species (Fig. 4). The pH-shift method concentrated the proteins most efficiently, and the concentration factor ranking order for the different seaweeds was S. latissima (4.0 times) > U. lactuca (2.6 times) > P. umbilicalis (2.2 times). The total fatty acid content in the P. umbilicalis pH-shift extract was slightly reduced compared to that in the crude seaweed; however, for both U. lactuca and S. latissima pH-shift extracts, it was concentrated, 2.2 and 1.6 times, respectively, compared to their crude seaweeds. The traditional method when applied to the seaweeds resulted in the generation of extracts with lower protein concentrations than in their respective crude seaweeds. The same was also true for the total fatty acids and ash contents achieved in the traditional extracts (see Fig. 4). Also with the ASE® method, the protein content and fatty acid content were lower in extracts generated compared to those of their respective crude seaweeds, while the ash content was highly concentrated in the extracts made with this method, for P. umbilicalis, 2.0 times greater; for U. lactuca, 1.97 times greater; and for S. latissima, 1.36 times greater.
a Total amino acids, b total fatty acids, and c ash content (% dw) of the three seaweed species (Porphyra umbilicalis, Ulva lactuca, and Saccharina latissima) measured in crude seaweed material, as well as in extracts obtained with three different methods (traditional, pH-shift, and ASE). Letters above bars indicate significant differences based on the Student-Newman-Keuls multiple comparison test. Error bars show +SEM (n = 3)
Amino acids of seaweed biomass and extracts
Total amino acids, when expressed on a dw basis, differed significantly (SNK, p < 0.05) between the three crude seaweed species and were ranked P. umbilicalis (31.8 ± 1.7%) > U. lactuca (19.6 ± 0.6%) > S. latissima (10.1 ± 0.2%). All the three seaweed species contained all the analyzed essential amino acids (EAA) histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, valine, and tyrosine, and they contributed to 39, 39, and 42% of the total amino acids for P. umbilicalis, U. lactuca, and S. latissima, respectively. Generally, the distribution of amino acids (Table 3) was quite similar in all three seaweed species. The most abundant amino acids were glycine, alanine, asparagine, leucine, and glutamate, with some internal differences in the order.
The total amount of amino acids in the produced extracts when expressed on a dw basis was for all three species highest when using the pH-shift technique. The achieved values here were P. umbilicalis 71.0 ± 3.7%, U. lactuca 51.2 ± 2.1%, and S. latissima 40.7 ± 0.5% (Fig. 4a), which is significantly (SNK, p < 0.05) higher than the amount of total amino acids in their respective crude seaweed biomasses. Out of the total amino acids, 42, 42, and 43% were EAA in the pH-shift-produced extracts for P. umbilicalis, U. lactuca, and S. latissima, respectively (Table 3). This corresponds to 30, 22, and 17% of the weight of respective extract (dw basis).
Neither the traditional method nor the ASE® method concentrated the amino acids compared to the crude biomass. In fact, the total amino acid content in the extracts made with both the traditional method and the ASE® method was significantly lower than in their corresponding crude biomass (SNK, p < 0.05).
Fatty acids of seaweed biomass and extracts
The pH-shift technique concentrated fatty acids (Fig. 4b) for both U. lactuca and S. latissima and the total fatty acid content in those extracts (5.0 ± 0.4% and 3.3 ± 0.2% on a dw basis, respectively) was significantly (SNK, p < 0.05) higher than that in their corresponding crude biomasses (2.3 ± 0% and 2.1 ± 0.4% of the dw, respectively) (Fig. 4b). However, there was no significant difference (SNK, p > 0.05) in the total fatty acid content between the pH-shift-produced extract and the crude biomass for P. umbilicalis (Fig. 4b). Overall, the total fatty acid content of all the traditionally made extracts and all the ASE extracts was significantly (SNK, p < 0.05) lower than that in their corresponding crude biomasses.
The fatty acids (Table 4) of both U. lactuca and S. latissima biomasses were more evenly distributed compared to those in P. umbilicalis where three fatty acids (C20:5 n3 (EPA), C16:0, and C20:3 n6) dominated and together constituted 82.8% of the total fatty acids.
EPA constituted 44.0 ± 0.0% of the total fatty acids in P. umbilicalis, corresponding to 1.1% of the dw. Thereafter, the most abundant fatty acids were C16:0 and C20:3 n6, which constituted 22.7 ± 0.0% and 16.1 ± 0.1% of the total amount of fatty acids, respectively. Those three fatty acids were also the most abundant ones in the three P. umbilicalis protein extracts. However, the order among them then changed.
The most abundant fatty acid in U. lactuca biomass was C18:3 n3, i.e., α-linolenic acid (ALA), constituting 25.0 ± 0.9% of the total fatty acids, which corresponds to 0.58% of the dw. Thereafter, the most abundant fatty acids were C16:0, C16:4 n3, and 18:4 n3, constituting 19.1 ± 0.9%, 13.3 ± 1.3%, and 12.6 ± 0.4% of the total fatty acids, respectively. Only 1.1 ± 0.1% of the fatty acids in U. lactuca was EPA. Also, for U. lactuca, the same fatty acids being the most abundant in the crude biomass were the most abundant ones in the extracts, with some minor changes in the ranking order. In contrast to P. umbilicalis and S. latissima (see below), U. lactuca biomass and its extracts contained docosapentaenoic acid (DPA), 22:5n3 in low amounts.
The four most abundant fatty acids in the S. latissima biomass were C20:5n3, C18:4n3, C16:0, and C20:4n6, constituting 18.9 ± 1.6%, 18.4 ± 0.7%, 16.8 ± 1.2%, and 14.1 ± 0.9% of the total amount fatty acids, respectively. However, in the S. latissima extracts, C16:0 was concentrated in favor of ALA and EPA. Also, C18:1 was concentrated in all the S. latissima extracts compared to the crude biomass. ALA constituted 8.5 ± 0.5% of the total fatty acid in crude S. latissima, but was lower in all extracts.
For all extracts from all three seaweed species, the relative amount of C16:0 was higher in the extracts compared to that in their corresponding crude seaweeds. Worth noting is that the fatty acid C20:0 was detected in all extracts from P. umbilicalis and U. lactuca, and in the pH-shift extract from S. latissima but in neither of the crude seaweeds.
Ash of seaweed biomass and extracts
The lowest ash content (Fig. 4c) was found in the extracts generated using the traditional method, and the levels here were significantly (SNK, p < 0.05) lower than those in their corresponding seaweeds. Also, the pH-shift-produced extracts had significantly (SNK, p < 0.05) lower ash content than the whole biomass. In contrast, the ash content in the ASE®-produced protein extracts was significantly (SNK, p < 0.05) higher than that in their respective crude seaweeds. The ASE®-generated protein extracts contained 46.3 ± 0.6%, 51.3 ± 0.7%, and 67.7% ash on a dw basis for P. umbilicalis, U. lactuca, and S. latissima (single analysis), respectively, compared to 23.2 ± 1.8%, 26.0 ± 0.7%, and 49.6 ± 4.3% ash dw in their respective crude biomasses.
Discussion
Following a systematic comparison of three different methods for extracting and concentrating proteins from a red, a green, and a brown seaweed species, this study illustrates that there are clear species differences regarding the response to different extraction principles, both in terms of protein yield, final protein concentration of extracts, and co-extraction of non-protein compounds. A secondary finding of the study was also that the protein analysis method affected the results more than expected, illustrating the challenge of measuring proteins in complex matrices like seaweeds which are rich, e.g., in pigments, carbohydrates, and salts.
Differences in protein yield determined using the Lowry method and analyses of total amino acids
Protein yields obtained in this study varied depending on the analysis method used (Lowry versus total amino acids) in accordance with a recent review paper by Maehre et al. (2018). However, the internal order between the different species/process method combinations was kept regardless of analysis method. For all combinations, with the exception of the U. lactuca ASE® extract, the yield was greater when data obtained with the Lowry method was used for calculations. For P. umbilicalis and U. lactuca crude biomass, both protein analysis methods gave similar protein levels; however, in the extracts, the Lowry method gave higher protein levels than total amino acids. This probably explains the higher protein yields obtained when using Lowry data to calculate the yields. For S. latissima, the protein levels of both the crude biomass and the extracts were higher when recorded using the Lowry method compared to those obtained with the total amino acid analysis method, resulting in more similar protein yield values with both analysis methods. Both the pigment content and the type of amino acids present (Legler et al. 1985) could affect the total protein results found using the Lowry method. Selective retention of certain amino acids and pigments in the extracts could affect the measured values recorded. The total amino acid method is more robust to such compositional differences and is generally used as a reference method when compiling nitrogen-to-protein conversion factors (i.e., Lourenço et al. 2002, Biancarosa et al. 2017). The amino acid method used in this study, however, lacks the ability to detect tryptophan which may result in a slight underestimation of the total amount of amino acids. According to the review of Fleurence (1999), tryptophan values of 0.3–3.0% of the total proteins have been reported for seaweeds.
Protein yield as a function of extraction method and species
Compared with earlier reports, the protein yield obtained with the traditional method for U. lactuca (19.6 and 10.9% with Lowry and total amount amino acids, respectively) was lower than that reported by Wong and Cheung (2001), where 36% protein yield was reported. In contrast to the traditional extraction method in the present study, Wong and Cheung (2001) added 2-mercaptoethanol, to increase the final yield, when re-suspending the pellet, something which removes food grade classification (Garcia-Vaquero et al. 2017). In addition, the pH was increased to pH 12 in this study (Wong and Cheung 2001) before ammonium sulfate precipitation was performed, and furthermore the residue was re-extracted five times instead of two, as was done here. Many other studies have examined the extraction of proteins from a variety of seaweed species, beyond those addresses in the present study, using variations of the traditional method (i.e., Wong and Cheung (2001), Kandasamy et al. (2012), Suresh Kumar et al. (2014) and Garcia-Vaquero et al. (2017)). However, since both compositional and structural differences between different seaweed species are substantial, direct comparisons of results are difficult.
To date, with the exception of the study carried out by Veide Vilg and Undeland (2017), nobody has reported values on seaweed protein yield using the pH-shift method or the ASE® method. Veide Vilg and Undeland (2017) only studied S. latissima, and in comparison with that study, the protein yield obtained here for S. latissima was higher (25% when assessed using the Lowry measurements versus 16% in their study). The latter (Veide Vilg and Undeland 2017) was, however, achieved when using a lower water to biomass ratio of 5.56:1(v/w) than the one used here 6:1 (v/w). It has clearly been shown how the addition of more water to the biomass (increased ratio of water/biomass) improves yield (Vareltzis and Undeland 2012, Veide Vilg and Undeland 2017) since the solubility of proteins is facilitated, and since the fractions removed during the pH-shift process become less concentrated. Also, Veide Vilg and Undeland (2017) did not apply a freeze-thawing step during the protein precipitation, the latter which was shown in this work to enhance precipitation for P. umbilicalis, U. lactuca, and S. latissima by 8, 4, and 14 percentage points, respectively. In terms of scaling up the pH-shift technique in an environmentally friendly manner, however, use of as low water volumes as possible and a minimum number of process steps is desirable which pin points a trade-off between scalability and efficiency during protein extraction. The costs for extra steps should be carefully weighed against the final value of the protein extract.
The “bio-refinery approach” using ASE® gave low total protein yields, especially for U. lactuca and S. latissima. Also, those extracts had lower protein concentrations than their respective crude biomasses. The reason for this may be explained by the fact that phlorotannins or phenols present in the raw seaweed biomass, that are removed during the second step of the ASE® method, bind to proteins and therefore also proteins were removed in the phlorotannin extraction step of the method. However, the fact that a lipid, a tannin/polyphenol, and a protein extract can be independently generated in a sequential manner is a positive attribute for this method. The extractability of proteins using both the pH-shift method and the ASE® method was higher for the red species (P. umbilicalis) than that for the green species (U. lactuca) which has also been seen by Wong and Cheung (2001). However, for the traditional extraction method, the opposite was seen. Regarding the brown species S. latissima, the extractability with the different methods was ranked as pH-shift > traditional > ASE®, the same order as for P. umbilicalis. However, the protein yield obtained for P. umbilicalis (4.1%) using ASE® was noticeably higher than that for S. latissima (0.7%). This clearly points to the diversity among different seaweed species regarding their response to different solvents, chemicals, and physical treatments. The cell wall differs between different seaweed species (Joubert and Fleurence 2008; Hurd et al. 2014), which could explain the variation in extractability between the three species. Also, the abundance and type of phenolic compounds vary largely (Sabeena Farvin and Jacobsen 2013), with the phlorotannins being exclusively found in the brown seaweed (Hurd et al. 2014), something which could explain the extremely low protein yield achieved when applying the ASE® method to S. latissima. Also, the ability of phlorotannins to precipitate proteins is pH-dependent (Stern et al. 1996). Precipitation of proteins caused by phlorotannins from either the brown seaweed Ecklonia radiata or the brown seaweed Carpophyllum maschalocarpum was highest between pH 2 and 7 (Stern et al. 1996). With the assumption that all phlorotannins have maximum precipitation ability at neutral, or acidic pHs, it is likely that this ability of phlorotannins will not affect the solubility of the protein in the first step of the pH-shift process. However, the appearance of phlorotannins could be an advantage in terms of the isoelectric precipitation step of the process. The presence of phlorotannins in the final extracts might, however, result in a bitter tasting protein extract, which should be evaluated in future studies.
Composition of protein extracts
The traditional method resulted in extracts with lower protein, ash, and total fatty acid content than their respective crude seaweeds. This indicates that the traditional method mainly concentrates the carbohydrates present in the raw material. The initial water extraction of proteins was probably poor since the adaption of the pH-shift process showed that the solubility of seaweed proteins at its native pH in water is only ~ 35% (Fig. 2).
Also with the ASE® method, the protein content and fatty acid content were lower in the resultant extracts compared to those of their respective crude seaweeds, while the ash content was highly concentrated. The initial de-fatting step explains the lower fat contents found and, as stated earlier, it is likely that the proteins were bound to, and removed together with the phlorotannins or phenols in the second extraction step of this method.
Amino acid profile
A crucial factor for all protein extracts is their nutritional value, i.e., their amino acid profile as well as the presence of other nutrients and tentative anti-nutrient factors, such as phenolic compounds, dietary fibers, or for example, trypsin inhibitors that could reduce the protein digestibility. In comparison to our study, none of the earlier mentioned studies, with the exception of the work carried out by Wong and Cheung (2001) and Garcia-Vaquero et al. (2017), have analyzed the amino acid profile of seaweed protein extracts, and none have analyzed the fatty acid pattern.
The amino acid analysis method used in this study lacks the ability to detect tryptophan; otherwise, all other essential amino acids were found in the three seaweed species examined (Table 3).
In the three pH-shift-produced protein extracts, the relative amount of EAA on a protein and dw basis (42–43% and 17–30%, respectively) was comparable to the values reported for soy flour, 40–48% on a protein basis and 15–24% on a dw basis (USDA Food Composition Databases 2018a; Friedman and Brandon 2001), respectively. Compared to egg white, which has 48% EAA on a protein basis and 39% on a dw basis (USDA Food Composition Databases 2018b), the seaweed extracts had somewhat lower values.
The reported relative amounts of EAA in the concentrates from Hypnea charoides, Hypnea japonica and U. lactuca produced by Wong and Cheung (2001) were slightly lower than the relative amounts found both in our pH-shift-produced and traditionally made extracts, and ranged between 36.2 and 40.2% of the total amino acids. Also, the relative amounts of EAA of a traditionally made protein extract from the brown seaweed Himanthalia elongata was slightly lower, 39.5% (Garcia-Vaquero et al. 2017) compared to the levels in our study.
It is recommended for adults that 11.1% of the protein should consist of EAA (Damodaran 2008), which here was fulfilled by all the crude seaweed biomasses, and also by all extracts, except for ASE® extracts generated from P. umbilicalis and U. lactuca. It should be stressed, however, that on a dw basis, the pH-shift-produced extracts gave several times more EAA than the traditional extracts; for P. umbilicalis, 5.3 times greater; for U. lactuca, 4.8 times greater; and for S. latissima, 20.9 times greater.
In the review by Friedman and Brandon (2001), it is stated that soy flour is deficient in methionine, since it only has methionine present at concentrations of 0.47–0.65% (dw) of the soy flour (USDA Food Composition Databases 2018a; Friedman and Brandon 2001). The corresponding numbers for methionine in the pH-shift-produced extracts were 0.71, 0.51, and 0.82% on a dw basis for P. umbilicalis, U. lactuca, and S. latissima, respectively, showing that seaweed-derived protein extracts may be a better source of methionine than soy meal, even if methionine and histidine were the two least abundant EAAs detected in the extracts generated with this method. Due to the sometimes co-reported values of methionine plus cysteine (since cysteine is a product of methionine (WHO 2002)), a comparison between studies and recommended values is obstructed. However, the recommended values for adults include that 1.7% of the proteins consumed should consist of methionine and cysteine together. The methionine and cysteine contents together compose 6.81% (Friedman and Brandon 2001) of the total proteins in soy. In the pH-shift-produced extracts from P. umbilicalis, U. lactuca and S. latissima, methionine and cysteine together constituted 3.4, 2.9, and 4.3% of the total amino acids present, respectively, which corresponds to 2.4, 1.5, and 1.8% of the extracts on a dw basis. Thus, for seaweed, as for other plant-based proteins (Friedman 1996), methionine seems to be the limiting EAA, even if levels of cysteine compensate for the joint values of methionine and cysteine.
Regarding histidine, the recommended value for adults is 1.6% on a protein basis, which means that the recommended values are fulfilled for the pH-shift-produced extracts from P. umbilicalis, U. lactuca and S. latissima, which contained 2.2, 2.2, and 2.1% histidine on a protein basis, respectively. The recommended values were, however, not fulfilled by the crude P. umbilicalis or U. lactuca biomass.
The relative quantity of glutamate, the amino acid giving rise to the typical umami taste (Yamaguchi and Ninomiya 2000; Ikeda 2002), ranged from ~ 8–11% of total amino acids in the crude seaweeds, and approximately the same relative abundance was seen in the traditionally produced (~ 7–9%) and the pH-shift-produced protein extracts (~ 7–9%). However, glutamate was highly concentrated in the ASE® protein extracts and in those extracts, glutamate constituted 23% (P. umbilicalis), 26% (U. lactuca), and 16% (S. latissima) of the total amount amino acids, corresponding to 4.9, 3.9, and 0.8% of the extracts on a dw basis, respectively. The high levels of both glutamate and ash (minerals) could make those extracts useful as alternative seasoning with a potential high umami taste.
Fatty acid profile
Co-extraction of other high-value food components such as fatty acids together with proteins is of high value for producing multi-functional protein extracts in the future. Provided they are maintained stable, the observed co-extraction of fatty acids along with proteins can be regarded an added value to the extracts when fatty acids are from the n3 series.
The content of EPA, one of the known health beneficial LC n3PUFAs (Lavie et al. 2009; Calder 2013), in the pH-shift extract of P. umbilicalis (0.8% on a dw basis) was around one third to one half the values found in fatty fish (herring and salmon) which according to the numbers from The National Food Agency database (2018a, b) contain 1.5% respectively 2.6% EPA on a dw basis (0.42 and 1.02% on a ww basis). Functional health claims can be made on products containing down to 40 mg EPA + DHA per 100 g product. From a functional foods perspective (Siró et al. 2008), it is thus an advantage that the fatty acids are not separated to a large extent from the proteins during pH-shift processing. However, both the lower amount of total fatty acids and the lower relative amount of EPA in the P. umbilicalis pH-shift extract compared to crude P. umbilicalis result in that the crude P. umbilicalis biomass contained 1.3 times more EPA than the P. umbilicalis pH-shift extract, 1.1% EPA on a dw basis. In U. lactuca and its extracts, EPA only constituted around 1% of the total fatty acids, but in contrast to P. umbilicalis, U. lactuca contained high levels of ALA, a precursor to the more beneficial long-chained n3 fatty acids. Also, in contrast to the pH-shift extracts from P. umbilicalis and S. latissima, 2.3% of the fatty acids in the pH-shift-produced extract from U. lactuca was docosapentaenoic acid (DPA), corresponding to 0.12% of the dw. This is more than double the amount (% dw) found in the crude U. lactuca biomass. A concentrating effect was thus seen, indicating that DPA could be bound to proteins in the seaweed. DPA is much less studied than EPA and DHA, but it is possessed that DPA has beneficial health effects (Kaur et al. 2011). Both the content of EPA and DPA, and in some cases DHA, which occasionally has been detected in S. latissima (Veide Vilg et al. 2015), thus make seaweeds and their protein extracts unique in comparison to agricultural crops and their products since land-based plants lack the natural ability to produce fatty acids with more than 18 carbons (Gill and Valivety 1997).
Ash content
Minerals, as estimated by ash, were largely reduced during the traditional process probably because of the de-salting dialysis step. Also, the pH-shift extracts had lower ash content than the whole biomasses, which is likely because of the dilution with water, i.e., salt is washed out during the process. However, the ASE® method was found to concentrate minerals in the resultant extracts. Together with the ability of the ASE® method to concentrate glutamate, the method could thus become useful as an alternative seasoning production method generating a product with high saltiness and umami taste. It should be stressed though that if there are potential heavy metals in the seaweed biomass, they will probably end up in this fraction. Worth to note is that no heavy metals were found in P. umbilicalis cultivated at the exact same destination as the biomasses used here (Wahlström et al. 2018). Of course, with any change in location of cultivation or species, both heavy metals and individual minerals need to be both determined and quantified.
Conclusion
This study showed that different species of seaweeds responded differently to the three protein extraction methods evaluated, but also, that some general trends could be seen. The traditional method gave the highest total protein yield for U. lactuca, but the protein concentration in all the extracts produced with this method was significantly lower than that recorded in their respective crude seaweeds. The same was also true for ash and total fatty acids, which were significantly lower than in their corresponding seaweed biomasses. This together with the time-consuming dialysis step and the high use of water might render upscaling of the traditional method difficult and unattractive for industry.
The ASE® technique concentrated the ash and glutamate content but resulted in extracts lower in both protein and lipid concentrations compared to their corresponding crude seaweeds. Therefore, those extracts would be interesting as salt replacers and/or umami enhancers, rather than as protein-enriching ingredients, with the abovementioned constraint.
The pH-shift techniques’ ability to significantly concentrate the proteins from all three seaweed species examined in this study and the fact that relatively high protein yields were achieved using this method make it a promising strategy for producing seaweed protein ingredients for food and feed use. The method has been scaled up with success for fish and other muscle sources, however, without the osmotic shock and freeze-induced precipitation steps. Future work still needs to be done to further increase the total protein yield achievable using this method and, for U. lactuca, to solve the problems reported here concerning the large variations in the precipitation step. Also, sensory evaluation and techno functionality testing of resulting extracts should be carried out to investigate their suitability as a potential food and/or feed ingredients.
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Acknowledgements
John Axelsson is thanked for help with examination of the effect of freezing during the pH-shift process on S. latissima.
Funding
The authors would like to thank the Swedish Foundation for Strategic Research (project no. 2820005) and the Swedish Research Council Formas (project no. 21210034) for funding this study.
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Harrysson, H., Hayes, M., Eimer, F. et al. Production of protein extracts from Swedish red, green, and brown seaweeds, Porphyra umbilicalis Kützing, Ulva lactuca Linnaeus, and Saccharina latissima (Linnaeus) J. V. Lamouroux using three different methods. J Appl Phycol 30, 3565–3580 (2018). https://doi.org/10.1007/s10811-018-1481-7
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DOI: https://doi.org/10.1007/s10811-018-1481-7