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Analysis of global metabolome by gas chromatography-mass spectrometry of Pyropia haitanensis stimulated with 1-octen-3-ol

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Abstract

1-Octen-3-ol is a major volatile metabolite produced by red seaweed Pyropia haitanensis under stresses. Using a metabolic profiling approach, we identified metabolites in P. haitanensis affected by 1-octen-3-ol treatment. The thalli were exposed to 1-octen-3-ol for 0.5 and 1 h. Using a non-targeted GC-MS analysis, 246 peaks in control and 1-octen-3-ol-treated P. haitanensis were detected. Among them, 72 metabolites were identified. Further statistical analysis revealed that these 72 metabolites covered different types of primary metabolism and secondary metabolism pathways, including organic acids, carbohydrates, amino acids, glycols, and fatty acids. PCA and PLS-DA analyses revealed that the metabolic composition differed between the control and 1-octen-3-ol-treated samples, and the main metabolites contributing to the dispersion were fatty acid, citric acid, some sugars, and amino acids. However, the metabolic compositions between two 1-octen-3-ol-treated samples (0.5 and 1 h) were similar. Pyropia haitanensis treated with 1-octen-3-ol showed reduced levels of certain free fatty acids and an increased level of monoacylglycerol. In addition, the synthesis of many amino acids and photosynthetic product galactosylglycerol was increased, along with some essential metabolites in the primary metabolic pathways, such as glycerol-3-phosphate, organic acids, and others. These results indicated that P. haitanensis recognized 1-octen-3-ol, which accelerated its primary metabolism to promote cell growth under stresses. These findings provided useful information on how algae respond to stress-induced signal.

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Acknowledgements

This study was supported by Zhejiang Open Foundation of the Most Important Subjects (xkzsc1419); LiDakSum Marine Biopharmaceutical Development Fund; National 111 Project of China; and the K.C. Wong Magna Fund of Ningbo University, Zhejiang 151 Talents Project, and the Open Fund of Zhejiang Provincial Top Key Discipline of Aquaculture in Ningbo University. We thank Bionovogene (Suzhou) for the help in data analysis.

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Authors and Affiliations

  1. Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Post Box 71, Ningbo, Zhejiang, 315211, China

    Qiuli Jian, Xiaojuan Zhu, Juanjuan Chen, Rui Yang, Qijun Luo & Haimin Chen

  2. Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, Zhejiang, 315211, China

    Zhujun Zhu & Xiaojun Yan

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  1. Qiuli Jian
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  2. Xiaojuan Zhu
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  3. Juanjuan Chen
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  4. Zhujun Zhu
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  5. Rui Yang
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  6. Qijun Luo
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  7. Haimin Chen
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Correspondence to Haimin Chen.

Electronic supplementary material

Table S1

Identified metabolites in Pyropia haitanensis by GC-MS (Normalized Peak area) (DOCX 26 kb).

Figure S1

KEGG metabolic pathway maps involving metabolites identified in this study. Metabolites were highlighted in red. Attached file: The KEGG metabolic pathways of the identified metabolites. Additional supporting information may be found in the online version of this article. (PDF 5026 kb).

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Jian, Q., Zhu, X., Chen, J. et al. Analysis of global metabolome by gas chromatography-mass spectrometry of Pyropia haitanensis stimulated with 1-octen-3-ol. J Appl Phycol 29, 2049–2059 (2017). https://doi.org/10.1007/s10811-017-1108-4

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