Abstract
Chaetomorpha antennina is a marine green alga found abundantly on the southern coast of India. This study explores the efficacy of C. antennina extracts in the management of diabetes using in vitro and in vivo models. The algal metabolites were extracted using various solvents and evaluated for inhibitory effect against key metabolic enzymes involved in blood sugar management and antioxidant property. Among the various extracts, methanolic extract of C. antennina (MECA) was found to be effective in all the inhibitory assays including, α-amylase (IC50 525.8 μg mL−1), α-glucosidase (IC50 121.3 μg mL−1), DPP-IV (IC50 24.92 μg mL−1) and antioxidant activity using DPPH (38 %) under in vitro conditions. In toxicity assays, MECA was nontoxic against mouse macrophage cells (J774) and red blood cells. Further, in vivo studies of MECA (250 mg kg−1 B.wt) on streptozotocin (STZ)-induced diabetic rats for a period of 28 days reduced the fasting blood glucose level to 39.97 % and that of positive control glibenclamide (0.25 mg kg−1 B.wt) was 73.05 % respectively. Serum cholesterol, triglycerides, ALT and AST were significantly decreased after 28 days of MECA treatment. GC-MS analysis of MECA showed the presence of ascorbic acid, octadecadienoate and fucosterol which were already identified for antidiabetic action. Overall, this study shows that the MECA exhibits antidiabetic activity in STZ-induced diabetic rats. Therefore, MECA can be considered for further studies to evaluate its mechanism for the effective management of diabetes.
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Acknowledgment
The authors wish to thank Department of Biotechnology, Government of India for their financial support (Grant No. BT/Bio-CARe/03/347/2010-11) and VIT University for providing all necessary facilities.
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Animals were maintained as per the principles and guidelines of the Institutional Animal Ethical Committee (IAEC) in accordance with Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) guidelines on animal care. All animal experiments were approved by the IAEC, VIT/IAEC/8th/23.
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Unnikrishnan, P.S., Jayasri, M.A. Antidiabetic studies of Chaetomorpha antennina extract using experimental models. J Appl Phycol 29, 1047–1056 (2017). https://doi.org/10.1007/s10811-016-0991-4
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DOI: https://doi.org/10.1007/s10811-016-0991-4