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Profiles of carotenoids and amino acids and total phenolic compounds of the red alga Pterocladiella capillacea exposed to cadmium and different salinities

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Abstract

The in vitro effect of cadmium (Cd) on apical segments of the red macroalga Pterocladiella capillacea was examined. Over a period of 7 days, the segments were cultivated with a combination of different salinities (25, 35, and 45 psu) and Cd concentrations ranging from 0.17 to 0.70 ppm. This study aimed to evaluate the effects of Cd exposure at various salinities on profiles of carotenoids amino acids and phenolic compounds. Carotenoid profile of control (0 ppm Cd) and Cd-treated plants of P. capillacea showed the presence of lutein, zeaxanthin, β-cryptoxanthin, α-carotene, trans-β-carotene and cis-β-carotene. Significant increase was found for almost all carotenoids, 0.35 ppm Cd + 25 psu, 0.70 ppm Cd + 25 psu, and 0 ppm Cd + 45 psu. Total phenolic compounds of P. capillacea had an overall decrease with treatments of Cd and 25 or 45 psu. Twenty essential amino acids were quantified in P. capillacea samples. At 0 ppm Cd and using baseline control of 35 psu, low and high salinity levels (25 and 45 psu, respectively) significantly altered total amino acid content. Apparently, salinity plays a greater role in amino acid response than Cd concentration. Therefore, more studies with intensified salinity stress over long-term exposure could confirm the major effect of the suggested treatment on this species.

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Acknowledgments

The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for the financial support of Éder C. Schmidt (process 473088/2013-4). Éder C. Schmidt holds a postdoctoral fellowship from CAPES. Zenilda L. Bouzon is a CNPq fellow. Fungyi Chow is a FAPESP fellow.

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Authors and Affiliations

  1. Postdoctoral Research of Postgraduate Program in Cell Biology and Development, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil

    Éder C. Schmidt

  2. Plant Cell Biology Laboratory, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil

    Marthiellen R. de L. Felix, Marianne G. Kreusch, Débora T. Pereira, Giulia B. Costa, Carmen Simioni, Luciane C. Ouriques & Zenilda L. Bouzon

  3. Institute of Bioscience, Department of Botany, University of São Paulo, 05508-090, São Paulo, SP, Brazil

    Fungyi Chow & Eny S. L. Floh

  4. Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil

    Fernanda Ramlov & Marcelo Maraschin

  5. Plant Physiology Laboratory, Department of Botany, Federal University of Santa Catarina, 88049-900, CP 476, Florianópolis, SC, Brazil

    Neusa Steiner

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  1. Éder C. Schmidt
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Correspondence to Éder C. Schmidt.

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Supplementary figure 1

Content of amino acids of P. capillacea after 7 days of exposure to cadmium and salinity treatments (n = 4, mean ± SD). a. 0 ppm Cd + 25 psu. b. 0 ppm Cd + 35 psu. c. 0 ppm Cd + 45 psu. d. 0.17 ppm Cd + 25 psu. e. 0.17 ppm Cd + 35 psu. f. 0.17 ppm Cd + 45 psu. g. 0.35 ppm Cd + 25 psu. h. 0.35 ppm Cd + 35 psu. i. 0.35 ppm Cd + 45 psu. j. 0.70 ppm Cd + 25 psu. k. 0.70 ppm Cd + 35 psu. l. 0.70 ppm Cd + 45 psu. Different letters indicate significant differences according to bifactorial ANOVA and Tukey’s test (p ≤ 0.05). (TIFF 79791 kb)

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Schmidt, É.C., Felix, M.R.d.L., Kreusch, M.G. et al. Profiles of carotenoids and amino acids and total phenolic compounds of the red alga Pterocladiella capillacea exposed to cadmium and different salinities. J Appl Phycol 28, 1955–1963 (2016). https://doi.org/10.1007/s10811-015-0737-8

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