Abstract
Climate change, fuel security and economics are creating a requirement for alternative, renewable fuels. Bioethanol is currently produced from land-based crops but in future marine biomass could be used as an alternative biomass source for bioethanol production. Macroalgae such as Laminaria spp. grow in abundance around the United Kingdom, reaching >4 m in length and containing up to 55% dry weight of the carbohydrates laminarin and mannitol. Using enzymes, these can be hydrolysed and converted to glucose and fructose, which in turn can be utilised by yeasts to produce ethanol. In previous studies on ethanol production from macroalgae, pre-treatment was at 65°C, pH 2 for 1 h prior to fermentation. This paper shows that these pre-treatments are not required for the fermentations conducted, with higher ethanol yields being achieved in untreated fermentations than in those with altered pH or temperature pre-treatments. This result was seen in fresh and defrosted macroalgae samples using Saccharomyces cerevisiae and 1 U kg−1 laminarinase.
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References
Black W (1950) The seasonal variation in weight and chemical composition of the common British Laminariaceae. J Mar Biol Assoc 29:45–72
Black W, Dewar E (1954) The properties of the algal chemicals. II Some derivatives of Laminarin. J Sci Food Agric 5:176–183, doi:10.1002/jsfa.2740050404
Boyer C, Hannah LC (2001) Kernel mutants of corn. In: Halluaer A (ed) Speciality corns. CRC, London, pp 1–32
Carlsson A, van Beilen J, Moller R, Clayton D (2007) Micro- and macro-algae: utility for industrial applications. In: Bowles D (ed) Outputs from the EPOBIO project. CNAP, University of York, York, UK
CEDUS Centre d’Edudes et de Documentation du Sucre (2008) Online document Understanding sugar—what is sugar. Sugar plants. www.sugarontheweb.com/ minihome.php?aff=1&id=1&idrub=1 Retrieved 22 July 2008
Department for Transport (2007) The renewable transport fuel obligations order rule number 3027. The Stationery Office, London
FAOSTAT (2008) ProdSTAT. Crops http://faostat.fao.org/site/567/default.aspx Accessed 22 July 2008
Gao K, McKinley K (1993) Use of macroalgae for marine biomass production and CO2 remediation: a review. J Appl Phycol 6:45–60, doi:10.1007/BF02185904
Hayward P, Nelson-Smith T, Shields C (1996) Sea shore of Britain and Northern Europe. Collins, London
Henry R, Kettlewell P (1996) Cereal grain quality. Chapman and Hall, London
Horn SJ (2000) PhD Thesis. Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway
Horn SJ, Aasen IM, Ostgaard K (2000a) Production of ethanol from mannitol by Zymobacter palmae. J Ind Microbiol Biotechnol 24:51–57, doi:10.1038/sj.jim.2900771
Horn SJ, Aasen IM, Ostgaard K (2000b) Ethanol production from seaweed extract. J Ind Microbiol Biotechnol 25:249–254, doi:10.1038/sj.jim.7000065
Juanich G (1988) Manual of running water fish culture 1 Eucheuma spp. Projected reports Cebu, Philippines, FAO
Kelly M, Dworjanyn S (2007) The potential of marine biomass for biofuel: a feasibility study with recommendation for further research. The Crown Estate, London
McHugh D (2003) A guide to the seaweed industry. Fisheries technical paper. FAO, Rome
Nelson TE, Lewis BA (1974) Separation and characterization of soluble and insoluble components of insoluble laminarin. Carbohydr Res 33:63–74, doi:10.1016/S0008-6215(00)82940-7
NEVC (2008) E85 flexfuel vehicles http://www.e85fuel.com/e85101/flexfuelvehicles.php Accessed on 22 July 2008
Nobe R, Sakakibara Y, Fukuda N, Yoshida N, Ogawa K, Suiko M (2003) Purification and characterization of laminaran hydrolases from Trichoderma viride. Biosci Biotechnol Biochem 67:1349–1357
Percival E, McDowell R (1967) Chemistry and enzymology of marine algal polysaccharides. Academic Press, London
Sze P (1998) A biology of the algae. McGraw-Hill, New York
The European Parliament and the Council of the European Union (2003) The promotion of use of biofuels or other renewable fuels for transport. Official Journal of the European Union, Directive 2003/30/EC, 8 May 2003. L 123/42–L 123/46
Wheals AE, Basso LC, Alves DM, Amorim HV (1999) Fuel ethanol after 25 years. Trends Biotechnol 17:482–487, doi:10.1016/S0167-7799(99)01384-0
Zvyagintseva TN, Shevchenko NM, Popivnich IB, Isakov VV, Scobun AS, Sundukova EV, Elyakova LA (1999) A new procedure for the separation of water-soluble polysaccharides from brown seaweeds. Carbohydr Res 322:32–39, doi:10.1016/S0008-6215(99)00206-2
Acknowledgements
This work was supported by the Engineering and Physical Sciences Research Council (EPSRC), grant number GR/S28204. Thanks to Maeve Kelly and Lars Brunner of the Scottish Association for Marine Sciences (SAMS), Oban for the supply of the frozen Sacchorina latissima samples.
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Adams, J.M., Gallagher, J.A. & Donnison, I.S. Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. J Appl Phycol 21, 569–574 (2009). https://doi.org/10.1007/s10811-008-9384-7
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DOI: https://doi.org/10.1007/s10811-008-9384-7