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Electrochemical determination of estradiol using a poly(l-serine) film-modified electrode

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Abstract

A thin film of poly(l-serine) was prepared via electropolymerization for the determination of trace levels of estradiol. In pH 5.0 phosphate buffer, l-serine was oxidized during the cyclic potential sweeps between −0.60 and 2.0 V, forming a thin film at the electrode surface. The electrochemical behavior of estradiol was investigated. The oxidation peak potential of estradiol shifts negatively at the poly(l-serine) film-coated glassy carbon electrode (GCE) compared with that at the bare GCE. Otherwise, the oxidation peak current greatly increases at the poly(l-serine) film-modified GCE. These phenomena suggest that the poly(l-serine) film exhibits catalytic activity towards the electrochemical oxidation of estradiol. Based on this, a sensitive, rapid and simple electrochemical method was proposed for the determination of estradiol. The limit of detection is evaluated to be 2.0 × 10−8 mol L−1. Finally, this method was successfully used to determine estradiol in blood serum.

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Authors and Affiliations

  1. Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Jinchun Song & Ji Yang

  2. College of Pharmacy, Wuhan University, Wuhan, 430072, China

    Xianming Hu

Authors
  1. Jinchun Song
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  2. Ji Yang
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  3. Xianming Hu
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Correspondence to Jinchun Song.

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Song, J., Yang, J. & Hu, X. Electrochemical determination of estradiol using a poly(l-serine) film-modified electrode. J Appl Electrochem 38, 833–836 (2008). https://doi.org/10.1007/s10800-008-9520-8

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  • DOI: https://doi.org/10.1007/s10800-008-9520-8

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