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Periplaneta americana extract ameliorates dextran sulfate sodium-induced ulcerative colitis via immunoregulatory and PI3K/AKT/NF-κB signaling pathways

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Abstract

Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) with a low cure rate. Periplaneta americana is a traditional American Cockroach and reportedly has potential therapeutic roles for UC treatment; however, its mechanisms remain unclear. To address this, we investigated the therapeutic effects and underlying molecular mechanisms of Ento-A, a Periplaneta americana extract, in a dextran sulfate sodium (DSS)-induced chronic and recurrent UC mouse model. Ento-A treatment decreased pro-inflammatory cytokine secretion, disease activity index (DAI), colon mucosa damage index (CMDI), histopathological scores (HS), and increased colon length. Additionally, Ento-A effectively increased interleukin-4 (IL-4), and forkhead transcription factor protein 3 (Foxp3) expression levels, while it abated interferon-γ (IFN-γ) and IL-17 levels in spleen lymphocytes. Conversely, in mesenteric lymph nodes, IL-4 and Foxp3 expression were decreased, while IFN-γ and IL-17 expression was increased. Furthermore, Ento-A blocked p-PI3K, p-AKT,*and p-NF-κB activation. In conclusion, Ento-A improved UC symptoms and exerted therapeutic effects by regulating immune responses and inhibiting PI3K/AKT/NF-κB signaling.

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All data generated or analyzed in this study are included in this manuscript and supplementary data.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NO. 81860742, 82060780, 81672305), Yunnan Fundamental Research Projects (2019FH001-003, 202001AT070021), and Innovative Team of Dali University for Digital Development of Medicinal Insects and Arachnids Resources (NO. ZKLX2020303).

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Author notes

  1. Lianli Ni and Qian Lu have contributed equally to this work.

Authors and Affiliations

  1. Cancer and Anticancer Drug Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China

    Lianli Ni, Lu Tao, Yun Yu & Ri Cui

  2. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, College of Pharmacy, Dali University, Yunnan, 671000, China

    Lianli Ni, Qian Lu, Miao Tang, Hairong Zhao, Chenggui Zhang & Heng Liu

  3. National-Local Joint Engineering Research Center of Entomoceutics, College of Pharmacy, Dali University, Yunnan, 671000, China

    Qian Lu, Miao Tang, Hairong Zhao, Chenggui Zhang, Xiumei Wu & Heng Liu

  4. School of Public Health, Dali University, Yunnan, 671000, China

    Xiumei Wu

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  1. Lianli Ni
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Ni, L., Lu, Q., Tang, M. et al. Periplaneta americana extract ameliorates dextran sulfate sodium-induced ulcerative colitis via immunoregulatory and PI3K/AKT/NF-κB signaling pathways. Inflammopharmacol 30, 907–918 (2022). https://doi.org/10.1007/s10787-022-00955-7

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