Abstract
Vasopressin possesses potent anti-inflammatory capacity. Phosphoinositide 3-kinase (PI3K) and its downstream activator Akt contribute to endogenous anti-inflammation capacity. We sought to elucidate whether PI3K is involved in mediating the anti-inflammation effects of vasopressin. Macrophages (RAW264.7 cells) were randomized to receive endotoxin, endotoxin plus vasopressin, or endotoxin plus vasopressin plus the nonselective PI3K inhibitor (LY294002) or the selective isoform inhibitor of PI3Kα (PIK-75), PI3Kβ (TGX-221), PI3Kδ (IC-87114), or PI3Kγ (AS-252424). Compared to macrophages treated with endotoxin, the concentrations of cytokines (tumor necrosis factor-α, interleukin-6) and chemokine (macrophage inflammatory protein-2) in macrophages treated with endotoxin plus vasopressin were significantly lower (all P < 0.05). The concentrations of phosphorylated nuclear factor-κB p65 (p-NF-κB p65) in nuclear extracts and phosphorylated inhibitor-κBα (p-I-κBα) in cytosolic extracts as well as NF-κB-DNA binding activity were also lower (all P < 0.05). Of note, except for macrophages treated with endotoxin plus vasopressin plus PIK-75, the concentrations of cytokines, chemokine, p-NF-κB p65, and p-I-κBα as well as NF-κB-DNA binding activity in macrophages treated with endotoxin plus vasopressin plus LY294002, TGX-221, IC-87114, or AS-252424 were significantly higher than those in macrophages treated with endotoxin plus vasopressin (all P < 0.05). In contrast, the phosphorylated Akt concentration in macrophages treated with endotoxin plus vasopressin was significantly higher than that in macrophages treated with endotoxin or in macrophages treated with endotoxin plus vasopressin plus LY294002, TGX-221, IC-87114, or AS-252424, but not PIK-75. These data confirmed that PI3K, especially the isoforms of PI3Kβ, PI3Kδ, and PI3Kγ, is involved in mediating the anti-inflammatory effects of vasopressin.
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References
Riddell, D.C., R. Mallonee, J.A. Phillips, J.S. Parks, L.A. Sexton, and J.L. Hamerton. 1985. Chromosomal assignment of human sequences encoding arginine vasopressin-neurophysin II and growth hormone releasing factor. Somatic Cell and Molecular Genetics 11: 189–95.
Sklar, A.H., and R.W. Schrier. 1983. Central nervous system mediators of vasopressin release. Physiological Reviews 63: 1243–80.
Landry, D.W., H.R. Levin, E.M. Gallant, R.C. Ashton Jr., S. Seo, D. D’Alessandro, et al. 1997. Vasopressin deficiency contributes to the vasodilation of septic shock. Circulation 5: 1122–5.
Sharshar, T., A. Blanchard, M. Paillard, J.C. Raphael, P. Gajdos, and D. Annane. 2003. Circulating vasopressin levels in septic shock. Critical Care Medicine 31: 1752–8.
Lin, I.Y., H.P. Ma, A.C. Lin, C.F. Chong, C.M. Lin, and T.L. Wang. 2005. Low plasma vasopressin/norepinephrine ratio predicts septic shock. American Journal of Emergency Medicine 23: 718–24.
Landry, D.W., H.R. Levin, E.M. Gallant, S. Seo, D. D’Alessandro, M.C. Oz, et al. 1997. Vasopressin pressor hypersensitivity in vasodilatory septic shock. Critical Care Medicine 25: 1279–82.
Baker, C.H., E.T. Sutton, Z. Zhou, and J.R. Dietz. 1990. Microvascular vasopressin effects during endotoxin shock in the rat. Circulatory Shock 30: 81–95.
Albert, M., M.R. Losser, D. Hayon, V. Faivre, and D. Payen. 2004. Systemic and renal macro- and microcirculatory responses to arginine vasopressin in endotoxic rabbits. Critical Care Medicine 32: 1891–8.
Kopel, T., M.R. Losser, V. Faivre, and D. Payen. 2008. Systemic and hepatosplanchnic macro- and microcirculatory dose response to arginine vasopressin in endotoxic rabbits. Intensive Care Medicine 34: 1313–20.
Dellinger, R.P., M.M. Levy, A. Rhodes, D. Annane, H. Gerlach, S.M. Opal, et al. 2013. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Medicine 39: 165–228.
Boyd, J.H., C.L. Holmes, Y. Wang, H. Roberts, and K.R. Walley. 2008. Vasopressin decreases sepsis-induced pulmonary inflammation through the V2R. Resuscitation 79: 325–31.
Peng, T.C., and C.J. Huang. 2013. Vasopressin inhibits endotoxin-induced upregulation of inflammatory mediators in activated macrophages. Tzu Chi Medical Journal 25: 150–4.
Chang, Y.Y., C.H. Yang, S.C. Wang, M.C. Kao, P.S. Tsai, and C.J. Huang. 2015. Vasopressin inhibits endotoxin binding in activated macrophages. The Journal of Surgical Research 197(2): 412–8.
Okkenhaug, K. 2013. Signaling by the phosphoinositide 3-kinase family in immune cells. Annual Review of Immunology 31: 675–704.
Guha, M., and N. Mackman. 2002. The phosphatidylinositol 3-kinase-Akt pathway limits lipopolysaccharide activation of signaling pathways and expression of inflammatory mediators in human monocytic cells. Journal of Biological Chemistry 277: 32124–32.
Tups, A., G.M. Anderson, M. Rizwan, R.A. Augustine, C. Chaussade, P.R. Shepherd, et al. 2010. Both p110alpha and p110beta isoforms of phosphatidylinositol 3-OH-kinase are required for insulin signaling in the hypothalamus. Journal of Neuroendocrinology 22: 534–42.
Gratacap, M.P., J. Guillermet-Guibert, V. Martin, G. Chicanne, H. Tronchère, F. Gaits-Iacovoni, et al. 2011. Regulation and roles of PI3Kβ, a major actor in platelet signaling and functions. Advances in Enzyme Regulation 51: 106–16.
Fung-Leung, W.P. 2011. Phosphoinositide 3-kinase delta (PI3Kδ) in leukocyte signaling and function. Cellular Signalling 23: 603–8.
Hirsch, E., G. Lembo, G. Montrucchio, C. Rommel, C. Costa, and L. Barberis. 2006. Signaling through PI3Kgamma: a common platform for leukocyte, platelet and cardiovascular stress sensing. Thrombosis and Haemostasis 95: 29–35.
Lee, P.Y., C.H. Yang, M.C. Kao, N.Y. Su, P.S. Tsai, and C.J. Huang. 2015. Phosphoinositide 3-kinase β, phosphoinositide 3-kinase δ, and phosphoinositide 3-kinase γ mediate the anti-inflammatory effects of magnesium sulfate. The Journal of Surgical Research 197: 390–7.
Tsai, P.S., C.C. Chen, P.S. Tsai, L.C. Yang, W.Y. Huang, and C.J. Huang. 2006. Heme oxygenase 1, nuclear factor E2-related factor 2, and nuclear factor kappaB are involved in hemin inhibition of type 2 cationic amino acid transporter expression and L-arginine transport in stimulated macrophages. Anesthesiology 105: 1201–10.
Chen, C.P., P.S. Tsai, and C.J. Huang. 2012. Anti-inflammation effect of human placental multipotent mesenchymal stromal cells is mediated by prostaglandin E2 via a myeloid differentiation primary response gene 88-dependent pathway. Anesthesiology 117: 568–79.
Gharbi, S.I., M.J. Zvelebil, S.J. Shuttleworth, T. Hancox, N. Saghir, J.F. Timms, et al. 2007. Exploring the specificity of the PI3K family inhibitor LY294002. The Biochemical Journal 404: 15–21.
Collins, C.B., D. Strassheim, C.M. Aherne, A.R. Yeckes, P. Jedlicka, and E.F. de Zoeten. 2014. Targeted inhibition of heat shock protein 90 suppresses tumor necrosis factor-α and ameliorates murine intestinal inflammation. Inflammatory Bowel Diseases 20: 685–94.
Manerba, M., L. Di Ianni, M. Govoni, M. Roberti, M. Recanatini, and G. Di Stefano. 2016. Lactate dehydrogenase inhibitors can reverse inflammation induced changes in colon cancer cells. European Journal of Pharmaceutical Sciences 96: 37–44.
Viviani, B., E. Corsini, M. Binaglia, L. Lucchi, C.L. Galli, and M. Marinovich. 2002. The anti-inflammatory activity of estrogen in glial cells is regulated by the PKC-anchoring protein RACK-1. Journal of Neurochemistry 83: 1180–7.
Holmes, C.L., D.W. Landry, and J.T. Granton. 2003. Science review: Vasopressin and the cardiovascular system part 1—receptor physiology. Critical Care 7: 427–34.
Acknowledgements
This work was supported by grants from the Mackay Junior College of Medicine, Nursing and Management (MKC104R9, awarded to W.C. Jan) and the Taipei Tzu Chi Hospital (TCRD-TPE-105-16, awarded to C.J. Huang).
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Jan, WC., Kao, MC., Yang, CH. et al. Phosphoinositide 3-Kinase Is Involved in Mediating the Anti-inflammation Effects of Vasopressin. Inflammation 40, 435–441 (2017). https://doi.org/10.1007/s10753-016-0489-x
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DOI: https://doi.org/10.1007/s10753-016-0489-x