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Involvement of Kallikrein-Kinin System on Cardiopulmonary Alterations and Inflammatory Response Induced by Purified Aah I Toxin from Scorpion Venom

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Abstract

Bradykinins are released from kininogen by kallikrein. They increase capillary lung permeability after their binding to β1 and especially β2 receptors before being metabolized by kininase enzyme. This study was performed to evaluate cardiopulmonary damages and inflammatory response on injected rats with Aah I toxin of scorpion venom and the involvement of Kallikrein-Kinin system in this pathogenesis. Obtained results revealed that Aah I toxin induces inflammatory cell infiltration accompanied by cellular peroxidase activities, a release of cytokine levels, pulmonary and myocardial damage, with altered metabolic activities and imbalanced redox status. Administration of aprotinin (bradykinin inhibitor) and especially icatibant (bradykinin β2 receptor antagonist) seemed to be able to protect animals against the toxicity of Aah I; nevertheless, the use of captopril (kininase II inhibitor) reduced partially some cardiac disorders. These findings indicate that the kallikrein-kinin system may contribute to the physiopathological effect and lung edema formation induced by toxin, which suggests a potential use of drugs with significant anti-kinin properties.

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Abbreviations

Aah:

Androctonus australis hector

Aah I:

Neurotoxins purified from Aah venom

ACE:

Angiotensin-converting enzyme

ACEI:

Angiotensin-converting enzyme inhibitor

Apro:

Aprotinine

Cap:

Captopril

Icati:

lcatibant

IL-1β:

Cytokines: Interleukin-1β

IL-6:

Interleukin-6

I-10:

Interleukin-10

MDA:

Malondialdehyde

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Authors and Affiliations

  1. USTHB, Faculty of Biological Sciences, Laboratory Cellular and Molecular Biology, Department Cellular and Molecular Biology, BP32, EL Alia, Bab Ezzouar, 16111, Algiers, Algeria

    Wafa Medjadba & Fatima Laraba-Djebari

  2. Aix-Marseille University, CNRS UMR 7286 CRN2M, IFR Jean-Roche, Faculté de Médecine Nord, Bd Pierre Dramard, 13916, Marseille, Cedex 20, France

    Marie-France Martin-Eauclaire

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  1. Wafa Medjadba
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  2. Marie-France Martin-Eauclaire
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  3. Fatima Laraba-Djebari
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Correspondence to Fatima Laraba-Djebari.

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Medjadba, W., Martin-Eauclaire, MF. & Laraba-Djebari, F. Involvement of Kallikrein-Kinin System on Cardiopulmonary Alterations and Inflammatory Response Induced by Purified Aah I Toxin from Scorpion Venom. Inflammation 39, 290–302 (2016). https://doi.org/10.1007/s10753-015-0249-3

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