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Recombinant interleukin-1 and tumor necrosis factor induce neutrophil migration “in vivo” by indirect mechanisms

  • Inflammation and Immunomodulation
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Abstract

The α and β forms of recombinant interleukin-1 (IL-1α and IL-1β) and of recombinant Tumor Necrosis Factor (TNFα and TNFβ) induced dose-dependent neutrophil migration into rat peritoneal cavities. Migration induced by both IL-1s showed a bell-shaped dose-response curve and IL-1β was 3-fold more potent than IL-1α. Pretreatment of the animals with dexamethasone or depletion of the peritoneal macrophage population, abolished the neutrophil migration induced by the four cytokines. “In vitro” stimulation of macrophage monolayers with IL-1β and the TNFs released a factor into the supernatant which, unlike these cytokines, induced neutrophil migration in dexamethasone pretreated animals. These results suggest that the neutrophil migration induced by IL-1α, IL-1β and TNFβ is not due to a direct effect on neutrophils, but occurs via the release of a chemotactic factors(s) from resident macrophages.

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Authors and Affiliations

  1. Department of Pharmacology of the Faculty of Medicine and of the faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    L. H. Faccioli, G. E. P. Souza, F. Q. Cunha &  S. H. Ferreira

  2. Department of Endocrinology, National Insitute for Biological Standards and Control, Blanche Lane, South Mimms, EN6 3QG, Potters Bar, Herts, UK

    S. Poole

Authors
  1. L. H. Faccioli
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  2. G. E. P. Souza
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  3. F. Q. Cunha
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  4. S. Poole
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  5. S. H. Ferreira
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Faccioli, L.H., Souza, G.E.P., Cunha, F.Q. et al. Recombinant interleukin-1 and tumor necrosis factor induce neutrophil migration “in vivo” by indirect mechanisms. Agents and Actions 30, 344–349 (1990). https://doi.org/10.1007/BF01966298

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  • DOI: https://doi.org/10.1007/BF01966298

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