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Dual Effects of Respiratory Syncytial Virus Infections on Airway Inflammation by Regulation of Th17/Treg Responses in Ovalbumin-Challenged Mice

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Abstract

We investigated the effects of respiratory syncytial virus (RSV) infections on ovalbumin (OVA)-challenged mice via regulation of Th17/Treg cell responses. BALB/c mice were challenged with OVA, followed by RSV infections twice. In OVA-challenged mice, the secretion of Th2/Th17-type cytokines, airway hyperresponsiveness and inflammation were significantly inhibited by initial RSV infection. Moreover, the in vivo findings demonstrated that initial RSV infection reversed the imbalance of Th17/Treg responses. In contrast, RSV re-infection strengthened Th2/Th17-type cytokine secretion, airway hyperresponsiveness, and inflammation, especially for lymphocyte infiltration in OVA-challenged mice. Meanwhile, RSV re-infection enhanced the imbalanced Th17/Treg responses. Upon all results reveal that RSV-induced respiratory infections may lead to dual effects pertaining to allergic airway inflammation by regulation of Th17/Treg responses.

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Abbreviations

OVA:

Ovalbumin

RSV:

Respiratory syncytial virus

AHR:

Airway hyperresponsiveness

BALF:

Bronchoalveolar lavage fluid

IL:

Interleukin

IFN-γ:

Interferon-γ

PBS:

Phosphate-buffered saline

i.p.:

Intraperitoneal

PFU:

Plaque-forming units

H&E:

Hematoxylin and eosin

PAS:

Periodic acid Schiff

BSA:

Bovine serum albumin

DNase I:

Deoxyribonuclease I

EDTA:

Ethylenediaminetetraacetic acid

FCM:

Flow cytometry

Ct:

Threshold cycles

PCR:

Polymerase chain reaction

RL :

Pulmonary resistance

Cdyn :

Lung dynamic compliance

Foxp3:

Forkhead box P3

RORγT:

Retinoid-related orphan receptor gamma t

HBSS:

Hanks’ balanced salt solution

FBS:

Fetal bovine serum

DMEM:

Dulbecco’s modified Eagle’s medium

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ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (No.81173390/H2902), the National Basic Science Program of China (No.2009CB523000), and the Shanghai Science and Technology Committee (No.09XD1400700). We gratefully acknowledge the technical assistance generously provided by Doc. Hongfu Li.

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  1. Lab of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai, 200040, China

    Jia Wang, Lingwen Kong, Qingli Luo, Bei Li, Jinfeng Wu, Baojun Liu, Xiao Wu & Jingcheng Dong

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  1. Jia Wang
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Wang, J., Kong, L., Luo, Q. et al. Dual Effects of Respiratory Syncytial Virus Infections on Airway Inflammation by Regulation of Th17/Treg Responses in Ovalbumin-Challenged Mice. Inflammation 37, 1984–2005 (2014). https://doi.org/10.1007/s10753-014-9931-0

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