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Anti-inflammatory Effects of Cavidine In Vitro and In Vivo, a Selective COX-2 Inhibitor in LPS-Induced Peritoneal Macrophages of Mouse

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ABSTRACT

Cavidine is an isoquinoline alkaloid which is isolated from Corydalis impatiens. In traditional Tibetan herb, C. impatiens has been widely used for treatment of skin injuries, hepatitis, cholecystitis, and scabies. The present study aimed to evaluate its anti-inflammatory effect and investigate the mechanisms underlying this anti-inflammatory action. We used different inflammation model animals and lipopolysaccharide (LPS)-induced murine peritoneal macrophages to examine the anti-inflammatory function of cavidine. Results indicated pretreatment with cavidine (i.p.) decreased xylene-induced ear edema, formaldehyde-induced paw edema, leukocyte number, and the level of nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-alpha (TNF-α) in acetic acid-induced peritonitis in mice. The data also demonstrated that cavidine significantly inhibited LPS-induced TNF-α, interleukin-6 (IL-6), and NO production in peritoneal macrophages. Moreover, cavidine regulated the expression of cyclooxygenase-2 (COX-2) instead of cyclooxygenase-1 (COX-1) at protein levels. These results suggested that cavidine is a selective COX-2 inhibitor which possesses an anti-inflammatory activity.

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REFERENCES

  1. Byeon, S.E., J.Y. Chung, Y.G. Lee, B.H. Kim, and K.H. Kim. 2008. In vitro and in vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae. Journal of Ethnopharmacology 119: 145–152.

    Article  PubMed  Google Scholar 

  2. Castardo, J.C., A.S. Prudente, J. Ferreira, C.L. Guimarães, F.D. Monache, M.F. Otuki, and D.A. Cabrini. 2008. Anti-inflammatory effects of hydroalcoholic extract and two biflavonoids from Garcinia gardneriana leaves in mouse paw oedema. Journal of Ethnopharmacology 118: 405–411.

    Article  CAS  PubMed  Google Scholar 

  3. Li, W., H. Huang, Y. Zhang, T. Fan, X. Liu, W. Xing, and X. Niu. 2013. Anti-inflammatory effect of tetrahydrocoptisine from Corydalis impatiens is a function of possible inhibition of TNF-α, IL-6 and NO production in lipopolysaccharide-stimulated peritoneal macrophages through inhibiting NF-κB activation and MAPK pathway. European Journal of Pharmacology 715: 62–71.

    Article  CAS  PubMed  Google Scholar 

  4. Serhan, C.N., and J. Savill. 2005. Resolution of inflammation: the beginning programs the end. Nature Immunology 6: 1191–1197.

    Article  CAS  PubMed  Google Scholar 

  5. Shohami, E., I. Ginis, and J.M. Hallenbeck. 1999. Dual role of tumor necrosis factor alpha in brain injury. Cytokine & Growth Factor Reviews 10: 119–130.

    Article  CAS  Google Scholar 

  6. White, M. 1999. Mediators of inflammation and the inflammatory process. Journal of Allergy and Clinical Immunology 103: S378–S381.

    Article  CAS  PubMed  Google Scholar 

  7. Yadav, S.K., B. Adhikary, S. Chand, B. Maity, S.K. Bandyopadhyay, and S. Chattopadhyay. 2012. Molecular mechanism of indomethacin-induced gastropathy. Free Radical Biology and Medicine 52: 1175–1187.

    Article  CAS  PubMed  Google Scholar 

  8. Natarajan, S.K., A. Mukhopadhya, A. Ramachandran, S. Amalanathan, G. Kurian, and K.A. Balasubramanian. 2007. Spontaneous bacterial peritonitis results in oxidative and nitrosative stress in ascitic fluid. Journal of Gastroenterology and Hepatology 22: 177–181.

    Article  CAS  PubMed  Google Scholar 

  9. Sautebin, L. 2000. Prostaglandins and nitric oxide as molecular targets for anti-inflammatory therapy. Fitoterapia 71: S48–S57.

    Article  CAS  PubMed  Google Scholar 

  10. Moeller, B., and P.M. Villiger. 2006. Inhibition of IL-1, IL-6, and TNF-alpha in immune-mediated inflammatory diseases. Springer Seminars in Immunopathology 27: 391–408.

    Article  CAS  Google Scholar 

  11. Xie, W.L., J.G. Chipman, D.L. Robertson, R.L. Erikson, and D.L. Simmons. 1991. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proceedings of the National Academy of Sciences 88: 2692–2696.

    Article  CAS  Google Scholar 

  12. Abdel-Aziz, A.A.M., K.E.H., ElTahir, and Y.A. 2011. Asiri synthesis, anti-inflammatory activity and COX-1/COX-2 inhibition of novel substituted cyclic imides. Part 1: Molecular docking study. European Journal of Medicinal Chemistry 46: 1648–1655.

  13. Soll, A.H., and D. McCarthy. 1999. NSAID-related gastrointestinal complications. Clinical Cornerstone 1(5): 42–56.

    Article  CAS  PubMed  Google Scholar 

  14. Niu, X., W. Li, H. Xu, X. Liu, and L. Qi. 2013. Simultaneous quantification of 11 isoquinoline alkaloids in Corydalis impatiens (Pall.) Fisch by HPLC. Journal of Separation Science 36: 2090–2095.

    Article  CAS  PubMed  Google Scholar 

  15. Niu, X.F., H.B. Xu, X. Liu, T. Fan, and L. Qi. 2013. Isoquinoline alkaloids from Corydalis impatiens. Chemistry of Natural Compounds 49: 187–189.

    Article  CAS  Google Scholar 

  16. Hosseinzadeh, H., M.H. Haddadkhodaparast, and A.R. Arash. 2003. Antinociceptive, antiinflammatory and acute toxicity effects of Salvia leriifolia Benth. seed extract in mice and rats. Phytotherapy Researc 17: 422–425.

    Article  Google Scholar 

  17. Cho, J.Y., K.U. Baik, J.H. Jung, and M.H. Park. 2000. In vitro anti-inflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa. European Journal of Pharmacology 398: 399–407.

    Article  CAS  PubMed  Google Scholar 

  18. Feng, W.G., Y.B. Wang, J.S. Zhang, and C.L. Li. 2002. cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in murine peritoneal macrophages. Cell Research 12: 331–337.

    Article  PubMed  Google Scholar 

  19. Pauwels, R., J. Balzarini, M. Baba, R. Snoeck, D. Schols, P. Herdewijn, and E. De Clercq. 1988. Rapid and automated tetrazolium-based colorimetric assay for the detection of anti-HIV compounds. Journal of virological method 20: 309–321.

    Article  CAS  Google Scholar 

  20. Chibli, L.A., K. Rodrigues, C.M. Gasparetto, N.C. Pinto, R.L. Fabri, E. Scio, and O.V. Sousa. 2014. Anti-inflammatory effects of Bryophyllum pinnatum (Lam.) Oken ethanol extract in acute and chronic cutaneous inflammation. Journal of Ethnopharmacology 154: 330–338.

    Article  PubMed  Google Scholar 

  21. Carlson, R.P., J. Chang, and A.J. Lewis. 1985. Modulation of mouse ear edema by cyclooxygenase and lipoxygenase inhibitors and other pharmacologic agents. Agents and Actions 17: 197–204.

    Article  CAS  PubMed  Google Scholar 

  22. Niu, X., T. Fan, W. Li, W. Xing, and H. Huang. 2012. The anti-inflammatory effects of sanguinarine and its modulation of inflammatory mediators from peritoneal macrophages. European Journal of Pharmacology 689: 262–269.

    Article  CAS  PubMed  Google Scholar 

  23. Banerjee, S., T.K. Sur, S. Mandal, P.C. Das, and S. Sikdar. 2000. Assessment of the anti-inflammatory effects of Swertia chirata in acute and chronic experimental models in male albino rats. Indian Journal of Pharmacology 32: 21–24.

    Google Scholar 

  24. Clementi, G., A. Caruso, V.M.C. Cutuli, A. Prato, N.G. Mangano, and M. Amico-Roxas. 1999. Antiinflammatory activity of adrenomedullin in the acetic acid peritonitis in rats. Life Sciences 65: PL203–PL208.

    Article  CAS  PubMed  Google Scholar 

  25. St-Onge, M., N. Flamand, J. Biarc, S. Picard, L. Bouchard, A.A. Dussault, C. Laflamme, M.J. James, G.E. Caughey, L.G. Cleland, P. Borgeat, and M. Pouliot. 2007. Characterization of prostaglandin E2 generation through the cyclooxygenase (COX)-2 pathway in human neutrophils. Biochimica et Biophysica Acta 1771: 1235–1245.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  26. Ribeiro, R.A., M.L. Vale, S.M. Thomazzi, A.B. Paschoalato, S. Poole, S.H. Ferreira, and F.Q. Cunha. 2000. Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. European Journal of Pharmacology 387: 111–118.

  27. Yun, K.J., J.Y. Kim, J.B. Kim, K.W. Lee, S.Y. Jeong, H.J. Park, and K.T. Lee. 2008. Inhibition of LPS-induced NO and PGE2 production by asiatic acid via NF-κB inactivation in RAW 264.7 macrophages: possible involvement of the IKK and MAPK pathways. International Immunopharmacology 8: 431–441.

    Article  CAS  PubMed  Google Scholar 

  28. Yoon, H.J., M.E. Moon, H.S. Park, H.W. Kim, S.Y. Im, J.H. Lee, and Y.H. Kim. 2008. Effects of chitosan oligosaccharide (COS) on the glycerol-induced acute renal failure in vitro and in vivo. Food and Chemical Toxicology 46: 710–716.

    Article  CAS  PubMed  Google Scholar 

  29. Moncada, S., R.M.J. Palmer, and E.A. Higgs. 1991. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological Reviews 43: 109–142.

    CAS  PubMed  Google Scholar 

  30. Nagai, H., H. Kumamoto, M. Fukuda, and T. Takahashi. 2003. Inducible nitric oxide synthase and apoptosis-related factors in the synovial tissues of temporomandibular joints with internal derangement and osteoarthritis. Journal of Oral and Maxillofacial Surgery 61: 801–807.

    Article  PubMed  Google Scholar 

  31. Dinarello, C.A. 1997. Proinflammatory and anti-inflammatory cytokines as mediators in the pathogenesis of septic shock. Chest 112: 321S–329S.

    Article  CAS  PubMed  Google Scholar 

  32. Fehsel, K., K.D. Kröncke, K.L. Meyer, H. Huber, V. Wahn, and V. Kolb-Bachofen. 1995. Nitric oxide induces apoptosis in mouse thymocytes. The Journal of Immunology 155: 2858–2865.

    CAS  PubMed  Google Scholar 

  33. Hong, S.K., H.J. Kim, C.S. Song, I.S. Choi, J.B. Lee, and S.Y. Park. 2012. Nitazoxanide suppresses IL-6 production in LPS-stimulated mouse macrophages and TG-injected mice. International Immunopharmacology 13: 23–27.

    Article  CAS  PubMed  Google Scholar 

  34. Subbaramaiah, K., N. Telang, J.T. Ramonetti, R. Araki, B. DeVito, B.B. Weksler, and A.J. Dannenberg. 1996. Transcription of cyclooxygenase-2 is enhanced in transformed mammary epithelial cells. Cancer Research 56: 4424–4429.

    CAS  PubMed  Google Scholar 

  35. Utar, Z., M.A. Majid, M.I. Adenan, M.F. Jamil, and T.M. Lan. 2011. Mitragynine inhibits the COX-2 mRNA expression and prostaglandin E2 production induced by lipopolysaccharide in RAW264.7 macrophage cells. Journal of Ethnopharmacology 136: 75–82.

    Article  CAS  PubMed  Google Scholar 

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ACKNOWLEDGMENTS

This research was financially supported in part by the Shanxi National Science Foundation of international cooperation projects (No. 2013KW26-02) to W.F. Li.

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Authors and Affiliations

  1. School of Pharmacy, Xi’an Jiaotong University, No. 76 Western Yanta Road, Xi’an City, Shaanxi Province, 710061, People’s Republic of China

    Xiaofeng Niu, Hailin Zhang, Weifeng Li, Qingli Mu, Huan Yao & Yu Wang

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  1. Xiaofeng Niu
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  2. Hailin Zhang
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Correspondence to Weifeng Li.

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Niu, X., Zhang, H., Li, W. et al. Anti-inflammatory Effects of Cavidine In Vitro and In Vivo, a Selective COX-2 Inhibitor in LPS-Induced Peritoneal Macrophages of Mouse. Inflammation 38, 923–933 (2015). https://doi.org/10.1007/s10753-014-0054-4

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