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Acanthoic Acid Inhibits LPS-Induced Inflammatory Response in Human Gingival Fibroblasts

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ABSTRACT

Periodontitis is a chronic disease that affects the gums and destroys connective tissue. Acanthoic acid (AA), a diterpene in Acanthopanax koreanum, has been reported to have anti-inflammatory activities. The aim of this study was to investigate the anti-inflammatory effects of AA on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). HGFs were treated with Porphyromonas gingivalis LPS in the presence or absence of AA. The production of inflammatory cytokines IL-8 and IL-6 were measured by ELISA. The expression of NF-κB and TLR4 were detected by Western blotting. The results showed that AA inhibited LPS-induced IL-8 and IL-6 production in a dose-dependent manner. In addition, AA inhibited LPS-induced TLR4 expression and NF-κB activation. In conclusion, AA inhibits LPS-induced inflammatory response in HGFs through inhibition TLR4-mediated NF-κB signaling pathway.

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Conflict of Interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Prosthodontics, Stomatology Hospital of Luzhou Medical College, Luzhou, Sichuan Province, 646000, China

    Cai Wei & He Xiaoping

  2. Department of Oral Medicine, Stomatology Hospital of Luzhou Medical College, Luzhou, Sichuan Province, 646000, China

    Chee Keong Tan & Jiang Junqiang

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  1. Cai Wei
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  2. Chee Keong Tan
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  3. He Xiaoping
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  4. Jiang Junqiang
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Correspondence to Jiang Junqiang.

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Wei, C., Tan, C.K., Xiaoping, H. et al. Acanthoic Acid Inhibits LPS-Induced Inflammatory Response in Human Gingival Fibroblasts. Inflammation 38, 896–901 (2015). https://doi.org/10.1007/s10753-014-0051-7

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