Skip to main content

Advertisement

SpringerLink
Log in

Serum Levels of the CC Chemokines CCL2, CCL5, and CCL11 in Food Allergic Children with Different Clinical Manifestations

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Food allergies (FA) are frequent in 8 % of children under 3 years old and approximately 2 % of adults. Chemokine are involved in various allergies such as FA. The present study was aimed to determine CCL2, CCL5, and CCL11 levels in FA. The study population of this cross-sectional study contained 63 patients suffering from FA and 100 healthy controls. Concentrations of CCL2, CCL5, CCL11, and IgE were measured by enzyme-linked immunosorbent assay (ELISA). Eosinophils were counted using Casy Ι cell counter + analyzer system model SCAREF system GmbH. Differences were considered significant at P < 0.05. Current results showed that FA patients had significantly elevated numbers of circulating periphery eosinophils than the disease-free controls. Serum IgE levels in FA patients were also higher than controls. We also showed that serum levels of CCL2 and CCL11 were significantly enhanced in FA patients compared to control but CCL5 was not detectable. Results of present study revealed that both CCL2 and CCL11 were more elevated in FA children suffering from anaphylaxis and urticaria than bronchial asthma and atopic dermatitis. These results also indicated that more increased levels of CCL2 and CCL11 were observed following consumption of cow’s milk and pistachio nuts. Overall, findings of the present study proposed that serum levels of CCL2 and CCL11 are elevated in FA and these may be considered as useful parameters in diagnosis of disorder. It is also possible to design treatments on the basis of blocking of chemokines expression by application of antibodies against them to overcome allergic complications in patients suffering from FA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Greener, M. 2000. Dissecting the molecular basis of food allergy. Molecular Medicine Today 6(9): 336.

    Article  PubMed  CAS  Google Scholar 

  2. Kim, J.W., et al. 2009. Morin inhibits Fyn kinase in mast cells and IgE-mediated type I hypersensitivity response in vivo. Biochemical Pharmacology 77(9): 1506–1512. Epub 2009 Feb 6.

    Article  PubMed  CAS  Google Scholar 

  3. Bischoff, S.C., et al. 1996. Quantitative assessment of intestinal eosinophils and mast cells in inflammatory bowel disease. Histopathology 28(1): 1–13.

    Article  PubMed  CAS  Google Scholar 

  4. Bischoff, S.C., and S. Kramer. 2007. Human mast cells, bacteria, and intestinal immunity. Immunology Reviews 217: 329–337.

    Article  CAS  Google Scholar 

  5. Bischoff, S.C. 2009. Physiological and pathophysiological functions of intestinal mast cells. Seminars in Immunopathology 31(2): 185–205. Epub 2009 Jun 17.

    Article  PubMed  CAS  Google Scholar 

  6. Frew, A.J. 1996. The immunology of respiratory allergies. Toxicology Letters 86(2–3): 65–72.

    Article  PubMed  CAS  Google Scholar 

  7. Piliponsky, A.M., et al. 2003. Non-IgE-dependent activation of human lung- and cord blood-derived mast cells is induced by eosinophil major basic protein and modulated by the membrane form of stem cell factor. Blood 101(5): 1898–1904. Epub 2002 Oct 10.

    Article  PubMed  CAS  Google Scholar 

  8. Drazen, J.M., J.P. Arm, and K.F. Austen. 1996. Sorting out the cytokines of asthma. The Journal of Experimental Medicine 183(1): 1–5.

    Article  PubMed  CAS  Google Scholar 

  9. Broide, D., and P. Sriramarao. 2001. Eosinophil trafficking to sites of allergic inflammation. Immunology Reviews 179: 163–172.

    Article  CAS  Google Scholar 

  10. Aminzadeh, F., et al. 2012. Differential expression of CXC chemokines CXCL10 and CXCL12 in term and pre-term neonates and their mothers. American Journal of Reproductive Immunology 68(4): 338–344.

    Article  PubMed  CAS  Google Scholar 

  11. Shinkai, A., et al. 1999. A novel human CC chemokine, eotaxin-3, which is expressed in IL-4-stimulated vascular endothelial cells, exhibits potent activity toward eosinophils. Journal of Immunology 163(3): 1602–1610.

    CAS  Google Scholar 

  12. Ahmadi, Z., M.K. Arababadi, and G. Hassanshahi. 2012. CXCL10 activities, biological structure, and source along with its significant role played in pathophysiology of type I diabetes mellitus. Inflammation. doi:10.1007/s10753-012-9555-1.

  13. Kitaura, M., et al. 1999. Molecular cloning of a novel human CC chemokine (Eotaxin-3) that is a functional ligand of CC chemokine receptor 3. Journal of Biological Chemistry 274(39): 27975–27980.

    Article  PubMed  CAS  Google Scholar 

  14. Ponath, P.D., et al. 1996. Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils. Journal of Experimental Medicine 183(6): 2437–2448.

    Article  PubMed  CAS  Google Scholar 

  15. Uguccioni, M., et al. 1997. High expression of the chemokine receptor CCR3 in human blood basophils. Role in activation by eotaxin, MCP-4, and other chemokines. Journal of Clinical Investigation 100(5): 1137–1143.

    Article  PubMed  CAS  Google Scholar 

  16. Plager, D.A., et al. 2010. Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitis. PLoS One 5(7): e11450.

    Article  PubMed  Google Scholar 

  17. Clark, A.T., and P.W. Ewan. 2004. Food allergy in childhood. Archives of Disease in Childhood 89(2): 197.

    Article  PubMed  CAS  Google Scholar 

  18. Simons, F.E. 2010. World Allergy Organization survey on global availability of essentials for the assessment and management of anaphylaxis by allergy-immunology specialists in health care settings. Annals of Allergy, Asthma & Immunology 104(5): 405–412.

    Article  Google Scholar 

  19. Blazquez, A.B., et al. 2010. A functional role for CCR6 on proallergic T cells in the gastrointestinal tract. Gastroenterology 138(1): 275–284. e1-4. Epub 2009 Sep 23.

    Article  PubMed  CAS  Google Scholar 

  20. Lamkhioued, B., et al. 1997. Increased expression of eotaxin in bronchoalveolar lavage and airways of asthmatics contributes to the chemotaxis of eosinophils to the site of inflammation. Journal of Immunology 159(9): 4593–4601.

    CAS  Google Scholar 

  21. Yamada, H., et al. 2000. Eotaxin in induced sputum of asthmatics: relationship with eosinophils and eosinophil cationic protein in sputum. Allergy 55(4): 392–397.

    Article  PubMed  CAS  Google Scholar 

  22. Taha, R.A., et al. 2001. Increased expression of the chemoattractant cytokines eotaxin, monocyte chemotactic protein-4, and interleukin-16 in induced sputum in asthmatic patients. Chest 120(2): 595–601.

    Article  PubMed  CAS  Google Scholar 

  23. Bottcher, M.F., et al. 2000. Chemoattractant factors in breast milk from allergic and nonallergic mothers. Pediatric Research 47(5): 592–597.

    Article  PubMed  CAS  Google Scholar 

  24. Taha, R.A., et al. 2000. Evidence for increased expression of eotaxin and monocyte chemotactic protein-4 in atopic dermatitis. Journal of Allergy and Clinical Immunology 105(5): 1002–1007.

    Article  PubMed  CAS  Google Scholar 

  25. Li, H., et al. 2007. Allergen-IgE complexes trigger CD23-dependent CCL20 release from human intestinal epithelial cells. Gastroenterology 133(6): 1905–1915. Epub 2007 Sep 25.

    Article  PubMed  CAS  Google Scholar 

  26. Hakimizadeh, E., et al. 2012. Association of -592 region of IL-10 polymorphisms with asthma in south-eastern Iranian patients. Clinical Laboratory 58(3–4): 267–271.

    PubMed  CAS  Google Scholar 

  27. Berkman, N., et al. 2001. Eotaxin-3 but not eotaxin gene expression is upregulated in asthmatics 24 h after allergen challenge. American Journal of Respiratory Cell and Molecular Biology 24(6): 682–687.

    Article  PubMed  CAS  Google Scholar 

  28. Kagami, S., et al. 2003. Significant elevation of serum levels of eotaxin-3/CCL26, but not of eotaxin-2/CCL24, in patients with atopic dermatitis: serum eotaxin-3/CCL26 levels reflect the disease activity of atopic dermatitis. Clinical and Experimental Immunology 134(2): 309–313.

    Article  PubMed  CAS  Google Scholar 

  29. Polzer, K., et al. 2008. Eotaxin-3 is involved in Churg–Strauss syndrome—a serum marker closely correlating with disease activity. Rheumatology (Oxford, England) 47(6): 804–808. Epub 2008 Apr 8.

    Article  CAS  Google Scholar 

  30. Fulkerson, P.C., et al. 2006. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proceedings of the National Academy of Sciences of the United States of America 103(44): 16418–16423. Epub 2006 Oct 23.

    Article  PubMed  CAS  Google Scholar 

  31. Matsuura, H., et al. 2009. Elevation of plasma eotaxin levels in children with food allergy. Nihon Rinshō Men’eki Gakkai Kaishi 32(3): 180–185.

    Article  PubMed  CAS  Google Scholar 

  32. Baggiolini, M., and C.A. Dahinden. 1994. CC chemokines in allergic inflammation. Immunology Today 15(3): 127–133.

    Article  PubMed  CAS  Google Scholar 

  33. Lukacs, N.W. 2001. Role of chemokines in the pathogenesis of asthma. Nature Reviews Immunology 1(2): 108–116.

    Article  PubMed  CAS  Google Scholar 

  34. Kaplan, A.P. 2001. Chemokines, chemokine receptors and allergy. International Archives of Allergy and Immunology 124(4): 423–431.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Authors of this manuscript thank all of the FA patients, their parents, and healthy controls which warmly attended in this study. This project was financially supported by a grant from Rafsanjan University of Medical Sciences.

Author information

Authors and Affiliations

  1. Department of ENT, Moradi Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Masood Radman

  2. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Gholamhossin Hassanshahi, Mojgan Noorozi Karimabad, Hossein Khorramdelazad & Zahra Ahmadi

  3. Social Determinants of Health Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Reza Vazirinejad

  4. Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mohammad Kazemi Arababadi

  5. Immunology Research Center, Buali Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Houshang Rafatpanah

  6. Department of Pathology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Fereshteh Iranmanesh

  7. Physiology and Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Elham Hakimizadeh

Authors
  1. Masood Radman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholamhossin Hassanshahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Reza Vazirinejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammad Kazemi Arababadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mojgan Noorozi Karimabad
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hossein Khorramdelazad
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Houshang Rafatpanah
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fereshteh Iranmanesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Elham Hakimizadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Zahra Ahmadi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zahra Ahmadi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Radman, M., Hassanshahi, G., Vazirinejad, R. et al. Serum Levels of the CC Chemokines CCL2, CCL5, and CCL11 in Food Allergic Children with Different Clinical Manifestations. Inflammation 36, 561–566 (2013). https://doi.org/10.1007/s10753-012-9577-8

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-012-9577-8

KEY WORDS

Search

Navigation