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Inhibition of Prostaglandin \({\text{F}}_{{{\text{2}}\alpha }} \) by Selective Cyclooxygenase 2 Inhibitors Accounts for Reduced Rat Leukocyte Migration

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Abstract

To investigate whether selective COX 2 inhibitors (celecoxib, rofecoxib) would play a role in a model of leukocyte migration in rats. Bacterial endotoxin (Escherichia coli LPS) was intraperitoneally injected at time zero in rats that were previously treated with unspecific and selective cyclooxygenase inhibitors. LPS induced a dose and time-dependent increase in leukocyte number, which was predominantly related to the presence of PMN neutrophils. Only rats treated with selective COX 2 inhibitors and indomethacin showed a significant reduction in leukocyte numbers following LPS administration. Prostaglandins E2 and \( {\text{F}}_{{2\alpha }} \) were injected into the peritoneum and the chemoatractant effect was studied. Only \( {\text{PGF}}_{{2\alpha }} \) was able to induce neutrophil increase following injection. Intraperitoneal reposition of \( {\text{PGF}}_{{2\alpha }} \) restored the abrogated leukocyte response to LPS, shown by rats pretreated with rofecoxib. It can be concluded that COX 2, through \( {\text{PGF}}_{{2\alpha }} \) release, is the isoform responsible for neutrophil recruitment in the rat model of LPS-induced inflammation.

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Acknowledgment

We wish to thank YS Bakhle for his suggestions regarding this project.

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Authors and Affiliations

  1. Laboratório de Inflamação e Dor, Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901, Brazil

    Gustavo Batista de Menezes, Webster Glayser Pimenta dos Reis, Júlia Maria Moreira Santos, Igor Dimitri Gama Duarte & Janetti Nogueira de Francischi

  2. Avenida Antonio Carlos, 6627-Pampulha, 31270-901, Belo Horizonte, Minas Gerais, Brazil

    Janetti Nogueira de Francischi

Authors
  1. Gustavo Batista de Menezes
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  2. Webster Glayser Pimenta dos Reis
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  3. Júlia Maria Moreira Santos
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  4. Igor Dimitri Gama Duarte
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  5. Janetti Nogueira de Francischi
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Correspondence to Janetti Nogueira de Francischi.

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Project supported by the Conselho Nacional de Pesquisa (CNPq).

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de Menezes, G.B., dos Reis, W.G.P., Santos, J.M.M. et al. Inhibition of Prostaglandin \({\text{F}}_{{{\text{2}}\alpha }} \) by Selective Cyclooxygenase 2 Inhibitors Accounts for Reduced Rat Leukocyte Migration. Inflammation 29, 163–169 (2005). https://doi.org/10.1007/s10753-006-9013-z

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