Abstract
Mesenchymal stem cells (MSCs) have several features that make them an attractive option for potentiating cartilage repair. Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation of SMSCs. However, the role of Sox4 in human SMSCs remains elusive. In the present study, we investigated the role of Sox4 in SMSCs through gain-of-function studies and found that Sox4 promoted cell proliferation and chondrogenesis. Furthermore, Sox4 could directly bind to the promoter of long noncoding RNA DANCR and increased its expression. Finally, knockdown of DANCR could reverse the stimulative effect of Sox4 on the proliferation and chondrogenesis of SMSCs. Taken together, our data highlights the pivotal role of Sox4 in the proliferation and differentiation of SMSCs.
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Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (Grant No. 81301582), Postdoctoral Science Foundation of Jiangsu Province (Grant No. 1402003B) and China Postdoctoral Science Foundation funded project (Grant No. 2015M572812).
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Lei Zhang and Shuo Chen have been contributed equally to this work.
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Zhang, L., Chen, S., Bao, N. et al. Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR. J Mol Hist 46, 467–473 (2015). https://doi.org/10.1007/s10735-015-9638-z
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DOI: https://doi.org/10.1007/s10735-015-9638-z