Skip to main content
SpringerLink
Log in

Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity

  • Published:
Journal of Molecular Histology Aims and scope Submit manuscript

Summary

With the marker of Paneth cells-lysozyme, secretory component (SC) immunoreactivity was demonstrated exclusively in Paneth cells of rat small intestine. The other types of epithelial cells (columnar, goblet, endocrine) were negative. On electron microscopic level, many SC-positive colloidal gold particles were found in rough endoplasmic reticulum, Golgi complexes, basal membrane and secretory granules of Paneth cells. These results suggest that SC is not a component of ingested immune complex, but a membrane receptor on Paneth cell. It may function as receptor for polymeric IgA and mediate its transport across the mucosal epithelium. Thus, Paneth cells are responsible for SC synthesis and participate in IgA-mediated acquired immunity in rat small intestine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ayabe T, Ashida T, Kohgo Y, Kono T (2004) The role of Paneth cells and their antimicrobial peptides in innate host defense. Trends Microbiol 12(8):394–398

    Article  PubMed  CAS  Google Scholar 

  • Brandtzaeg P (1974) Mucosal and glandular distribution of immunoglobulin component: differential localization of free and bound SC in secretory epithelial cells. J Immunol 112(4):1553–1559

    PubMed  CAS  Google Scholar 

  • Brandtzaeg P (2003) Role of secretory antibodies in the defence against infections. Int J Med Microbiol 293(1):3–15

    Article  PubMed  CAS  Google Scholar 

  • Brown WR, Isobe Y, Nakane PK (1976) Studies on translocation of immunoglobulins across intestinal epithelium. II. Immunoelectron-microscpic localization of immunoglobulins and secretory component in human intestinal mucosa. Gastroenterology 71(9):985–995

    PubMed  CAS  Google Scholar 

  • Brown WR, Isobe K, Nakane PK, Pacini B (1977) Studies on translocation of immunoglobulins across intestinal epithelium. IV. Evidence for binding of IgA and IgM to secretory component in intestinal epithelium. Gastroenterology 73(6):1333–1339

    PubMed  CAS  Google Scholar 

  • Brown WR, Kloppel TM (1989) The liver and IgA: Immunological, cell biological and clinical implications. Hepatology 9(5):763–784

    Article  PubMed  CAS  Google Scholar 

  • Erlandsen SL, Chase DG (1972a) Paneth cell function: phagocytosis and intracellular digestion of intestinal microorganisms. I. Hexamita muris. J Ultrastruct Res 41(3):296–318

    Article  CAS  Google Scholar 

  • Erlandsen SL, Chase DG (1972b) Paneth cell function: phagocytosis and intracellular digestion of intestinal microorganisms. II. Spiral microorganism. J Ultrastruct Res 41(3):319–333

    Article  CAS  Google Scholar 

  • Erlandsen SL, Parsons JA, Taylor TD (1974) Ultrastructural immunocytochemical localization of lysozyme in the Paneth cells of man. J Histochem Cytochem 22(6):401–413

    PubMed  CAS  Google Scholar 

  • Erlandsen SL, Parsons JA, Cambier J, Bulter J (1975) Immunocytochemical distribution of lysozyme and immunoglobulin A in rat small intestine and its relationship to Paneth cells function. J Histochem Cytochem 23(4):323–324

    Google Scholar 

  • Erlandsen SL, Rodning CB, Montero C, Parsons JA, Lewis EA, Wilson ID (1976) Immunocytochemical identification and localization of immunoglobulin A within Paneth cells of the rat small intestine. J Histochem Cytochem 24(10):1085–1092

    PubMed  CAS  Google Scholar 

  • Johansen FE, Brandtzaeg P (2004) Transcriptional regulation of the mucosal IgA system. Trends Immunol 25(3):150–157

    Article  PubMed  CAS  Google Scholar 

  • Mestecky J, Lue C, Russell MW (1991) Selective transport of IgA. Cellular and molecular aspects. Gastroenterol Clin North Am 20(3):441–471

    PubMed  CAS  Google Scholar 

  • Mostov KE (1994) Transepithelial transport of immunoglobulins. Annu Rev Immunol 12:63–84

    Article  PubMed  CAS  Google Scholar 

  • Poger ME, Lamm ME (1974) Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. J Exp Med 139(3):629–642

    Article  PubMed  CAS  Google Scholar 

  • Rodning CB, Erlandsen SL, Wilson ID (1975) Localisation of immunoglobulin in human small intestinal Paneth cells. Clin Res 23:520A

    Google Scholar 

  • Rodning CB, Wilson ID, Erlandsen SL (1976) Immunoglobulins within human small-intestinal Paneth cells. Lancet 1(7967):984–987

    Article  PubMed  CAS  Google Scholar 

  • Satoh Y, Ishikawa K, Tanaka H, Ono K (1986) Immunohistochemical observations of immunoglobulin A in the Paneth cells of germ-free and formerly-germ-free rats. Histochemistry 85(3):197–201

    Article  PubMed  CAS  Google Scholar 

  • Selsted ME, Miller SI, Henschen AH, Ouellette AJ (1992) Enteric defensins: antibiotic peptide components of intestinal host defense. J Cell Biol 118(4):929–936

    Article  PubMed  CAS  Google Scholar 

  • Senegas-Balas F, Balas D, Verger R, de Caro A, Figarella C, Ferrato F, Lechene P, Bertrand C, Ribet A (1984) Immunohistochemical localization of intestinal phospholipase A2 in rat Paneth cells. Histochemistry 81(6):581–584

    Article  PubMed  CAS  Google Scholar 

  • Tao KZ, Tang QJ, Liu Y, Kang ZM, Jiang CL (2004) Changes of lysozyme and secretory component in ileum of 72 h sleep-deprived rat. Chinese Journal of Behavioral Medical Science 13(5):491–493

    Google Scholar 

Download references

Acknowledgements

We are greatly indebted to Ms. Kang ZM and Mrs. Yan YB for excellent technical assistance. This study was supported by fund for young scholars from Second Military Medical University (P. R. China).

Author information

Authors and Affiliations

  1. Department of Nautical Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, P. R. China

    Qing-Juan Tang, Kai-Zhong Tao,  Yun-Liu, Xue-Jun Sun & Chun-Lei Jiang

  2. Department of Molecular Pharmacology, Marine Drug and Food Institute, Ocean University of China, Qingdao, P. R. China

    Qing-Juan Tang & Mei-Yu Geng

Authors
  1. Qing-Juan Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kai-Zhong Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yun-Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xue-Jun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mei-Yu Geng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chun-Lei Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kai-Zhong Tao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, QJ., Tao, KZ., Yun-Liu et al. Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity. J Mol Hist 36, 331–335 (2005). https://doi.org/10.1007/s10735-005-9003-8

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10735-005-9003-8

Keywords

Search

Navigation