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PpVIN2, an acid invertase gene family member, is sensitive to chilling temperature and affects sucrose metabolism in postharvest peach fruit

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Abstract

We previously reported that expression and activity of acid invertases (AI) are increased in peach fruit under chilling stress. In order to determine which AI genes respond to chilling stress, seven AI genes, two vacuolar invertases (VINs) and five cell wall-bound invertases (CWINs), were identified and cloned. The predicted amino acid sequences of the genes contain conserved sites characteristic of plant AIs such as NDPNG/A, the sucrose-binding site, and MWECV/P, a cysteine catalytic motif. Using gene-specific primers, the expression of each gene was measured in ‘Baifeng’ and ‘Yulu’ peach fruits stored at 0, 5, 10 and 20 °C. Of the seven genes, expression of PpVIN2 was the most affected by chilling stress; the largest increases were in fruit stored at 5 °C, up to 17-fold in ‘Baifeng’ fruit, and up to 280-fold in ‘Yulu’ fruit. Overall, VIN activity was much higher than CWIN activity in stored peach fruit. In both cultivars reducing sugar content increased significantly and sucrose content decreased gradually during storage at 5 °C relative to other temperatures, and was accompanied by severe chilling injury symptoms. Thus, PpVIN2 appears to be induced by chilling and may play an important role in sucrose metabolism in peach fruit subjected to cold storage.

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Acknowledgements

This study was sponsored by the National Natural Science Foundation of China (No. 31671903), the Nature Science Foundation of Zhejiang Province (No. LR15C200002), and the K. C. Wong Magna Fund in Ningbo University.

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Authors and Affiliations

  1. Department of Food Science and Engineering, Ningbo University, Ningbo, China

    Xingxing He, Yingying Wei, Jingyu Kou, Feng Xu & Xingfeng Shao

  2. School of Science and Health, Western Sydney University, Penrith, NSW, Australia

    Zhonghua Chen

  3. China-Australia Joint Center for Food and Health, Ningbo University & Western Sydney University, Ningbo, China

    Xingxing He, Yingying Wei, Jingyu Kou, Feng Xu & Xingfeng Shao

  4. Australia-China Joint Center for Food and Health, Western Sydney University & Ningbo University, Penrith, NSW, Australia

    Zhonghua Chen

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  1. Xingxing He
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Correspondence to Xingfeng Shao.

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He, X., Wei, Y., Kou, J. et al. PpVIN2, an acid invertase gene family member, is sensitive to chilling temperature and affects sucrose metabolism in postharvest peach fruit. Plant Growth Regul 86, 169–180 (2018). https://doi.org/10.1007/s10725-018-0419-z

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  • DOI: https://doi.org/10.1007/s10725-018-0419-z

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