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Altered tetrapyrrole metabolism and transcriptome during growth-promoting actions in rice plants treated with 5-aminolevulinic acid

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Abstract

As a precursor of chlorophyll, 5-aminolevulinic acid (ALA) acts as a growth-promoting factor in plants, but little is known of molecular basis of plant growth action. The exogenous supply of an optimal concentration of 75 µM ALA without photooxidative risks, as indicated by a negligible change in conductivity, increased dry shoot biomass of rice up to 16 % after 10 days of ALA treatment, compared to the untreated control. Although rice plants treated with ALA gradually increased levels of ALA-synthesizing capacity during 30 h of the treatment, a noticeable increase in chlorophyll content was not observed. Levels of protoporphyrin IX and Mg–porphyrins were not significantly changed 6 h after ALA treatment and then decreased 30 h after the treatment, while transcript levels of most biosynthetic genes in tetrapyrrole pathway almost kept constant or slightly decreased. In microarray analysis of ALA-treated rice plants, categories of significantly up-regulated transcripts highlight particular biological processes involved in cell cycle, transcription factors, posttranscriptional regulation, and metabolisms of macromolecules. Our results demonstrate that the regulation of tetrapyrrole biosynthesis did not contribute significantly to growth enhancement in ALA-treated rice. We propose that the elevated status of ALA in plant cells alters the transcriptional control of genes involved in physiological processes vital for plant growth, thereby contributing to enhancement of plant biomass.

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Abbreviations

ALA:

5-Aminolevulinic acid

Mg–Proto IX:

Mg–protoporphyrin IX

Mg–Proto IX ME:

Mg–protoporphyrin IX methyl ester

Pchlide:

Protochlorophyllide

Proto IX:

Protoporphyrin IX

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Acknowledgments

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (Ministry of Education, Science and Technology) (NRF-2009-0076123 and NRF-2010-0005635).

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Authors and Affiliations

  1. BK21 Plus KNU Creative Bioresearch Group, School of Life Sciences and Biotechnology, Kyungpook National University, Daegu, 702-701, Korea

    Haianh Nguyen, Hyeng-Soo Kim & Sunyo Jung

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  1. Haianh Nguyen
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  2. Hyeng-Soo Kim
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  3. Sunyo Jung
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Correspondence to Sunyo Jung.

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Nguyen, H., Kim, HS. & Jung, S. Altered tetrapyrrole metabolism and transcriptome during growth-promoting actions in rice plants treated with 5-aminolevulinic acid. Plant Growth Regul 78, 133–144 (2016). https://doi.org/10.1007/s10725-015-0080-8

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