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The role of ethylene and brassinosteroids in the control of sex expression and flower development in Cucurbita pepo

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Abstract

In this paper we compare the sensitivity of different squash genotypes to ethylene and brassinosteroids by studying the effects of different ethylene and brassinosteroid treatments on the sexual expression and flower development of different C. pepo genotypes: Bolognese (Bog) and Vegetable Spaghetti (Veg), two contrasting lines for ethylene production and sensitivity, as well as Cora, a standard commercial hybrid. Results have demonstrated that ethylene has a much greater effect on sexual expression and flower development in C. pepo than brassinosteroids. Ethephon increases the number of female flowers per plant and reduces the first male phase of development, while treatments with the ethylene inhibitors AVG and STS reduce the number of female flowers per plant and expand the first male phase of development. The differential response observed between genotypes appears to be related to ethylene production and sensitivity. Bog, which produces more ethylene and is more sensitive to this hormone, responded much better to AVG and STS, reducing the number of female flowers per plant, while Veg, which is characterised by lower production of and sensitivity to ethylene, responded better to ethephon by reducing the first male phase of development and increasing the number of female flowers per plant. The differential abortion of female and male flowers in ethephon, AVG and STS treatments, as well as the occurrence of bisexual flowers in the AVG and STS treated plants of the more ethylene sensitive genotypes, demonstrate that ethylene is also involved in the development of female flowers. Female flower buds require a minimal level of ethylene not only to complete their development and maturation without a premature abortion, but also to arrest the development of stamens in the third whorl and to promote the appropriate growth of the carpels. On the contrary, the role of brassinosteroids in the sexual expression of C. pepo was not so evident. The application of brassinazole, an inhibitor of brassinosteroid biosynthesis slightly changes the production of ethylene in the three analysed genotypes, but those changes have little effect on their sexual phenotypes, and they do not alter the development of the unisexual flowers.

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Acknowledgments

This work was supported by the Ministerio de Ciencia e Innovación of Spain [AGL2005-06677-CO2 and AGL2008-05619-C02-02/ALI], and by the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain [CVI-02617]. We would like to thank Dr. Miriam Nuñez from the research institute INCA of Cuba for supplying the brassinazole used in this study.

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  1. Departamento de Biología Aplicada. Escuela Politécnica Superior, Universidad de Almería, 04120, Almería, Spain

    Susana Manzano, Cecilia Martínez, Zoraida Megías & Manuel Jamilena

  2. Departamento de Biotecnología, IFAPA de Almería. Autovía del mediterráneo, La Mojonera, Almería, Spain

    Pedro Gómez

  3. Departamento de Fisiología Vegetal, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain

    Dolores Garrido

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  1. Susana Manzano
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  3. Zoraida Megías
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  5. Dolores Garrido
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  6. Manuel Jamilena
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Correspondence to Manuel Jamilena.

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Manzano, S., Martínez, C., Megías, Z. et al. The role of ethylene and brassinosteroids in the control of sex expression and flower development in Cucurbita pepo . Plant Growth Regul 65, 213–221 (2011). https://doi.org/10.1007/s10725-011-9589-7

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  • DOI: https://doi.org/10.1007/s10725-011-9589-7

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